Functional expression and activity of the recombinant antifungal defensin PvD1r from Phaseolus vulgaris L. (common bean) seeds

Conclusion:
Our results indicate that PvD1r retains full biological activity after recombinant production, enterokinase cleavage and purification. Additionally, our results from the antimicrobial assay, the microscopic analysis and the PvD1r-FITC labeling assays corroborate each other and lead us to suggest that the target of PvD1 in C. albicans cells is the sphingolipid glucosylceramide.

Source: BMC Biochemistry - April 1, 2014 Category: Biochemistry Authors: Érica de O MelloIzabela dos SantosAndré de O CarvalhoLuísa de SouzaGonçalo de Souza-FilhoViviane do NascimentoOlga MachadoUmberto ZottichValdirene Gomes Source Type: research