VEGF and VEGFR2 in dentigerous cysts associated with impacted third molars
Summary: The aims of this study were to determine the presence and distribution of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor-2 (VEGFR2) in dentigerous cysts compared with normal dental follicles as a control tissue and to evaluate endothelial cells and proliferating cells as indicators of angiogenic activity in these tissues.
Twenty specimens histologically diagnosed as dentigerous cysts and 20 dental follicle specimens were included. Immunohistochemistry (IHC) using anti-VEGF and anti-VEGFR2 antibodies stained for the growth factor and its receptor, while anti-CD34 and anti-...
Source: Pathology - July 12, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
SWI/SNF complex deficiency and mismatch repair protein expression in undifferentiated and dedifferentiated endometrial carcinoma
Summary: Undifferentiated endometrial carcinoma (UEC) is a relatively uncommon but clinically aggressive uterine malignancy. In common with a subset of poorly differentiated carcinomas arising in other sites, UEC may exhibit rhabdoid morphology and be associated with a low-grade tumour component (dedifferentiated carcinoma). Recent studies have implicated inactivation of the SWI/SNF complex subunits in the aforementioned extrauterine tumours. Therefore we have examined INI1 (SMARCB1), BRG1 (SMARCA4), and BAF250a (ARID1A) immunostaining, and also expression of the DNA mismatch repair (MMR) proteins MLH1, PMS2, MSH2 and MSH6...
Source: Pathology - July 12, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
BRAF p.Val600Glu (V600E) mutation detection in thyroid fine needle aspiration cell block samples: a feasibility study
Summary: Assessing BRAF mutation status in thyroid fine needle aspiration (FNA) cytology samples by both immunohistochemistry (IHC) and molecular methods has been documented in recent literature. We aim to highlight issues relating to quality and quantity of cellular material and DNA extracted from cell block samples.
BRAF mutation status was assessed by both molecular and IHC methods in cell block material from thyroid FNA samples over a range of diagnostic categories, and correlated with available follow-up resection specimens.
Of 39 samples there were 14 cases with ‘inconclusive’ cytology (Bethesda diagnostic catego...
Source: Pathology - July 12, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
The secondary Müllerian system, field effect, BRCA, and tubal fimbria: our evolving understanding of the origin of tubo-ovarian high-grade serous carcinoma and why assignment of primary site matters
Summary: It has long been held that most epithelial ovarian carcinomas arise from the ovarian surface epithelium. Theories on origin were based on the assumption that there was a common cell of origin for all ovarian carcinoma histotypes, and that these histotypes were closely related and frequently admixed. It is now recognised that the histotypes are distinct diseases. Recent studies on early, organ-confined, non-uterine high-grade serous carcinoma (HGSC) have led to a change in our understanding of their anatomical site of origin. These studies were initially on patients at high risk of developing HGSC but more recently...
Source: Pathology - July 12, 2015 Category: Pathology Tags: Reviews Source Type: research
Recently described entities in the gynaecological tract
Summary: In recent years there have been significant advances in our understanding of female genital tract tumours due to the fact that new molecular abnormalities and translocations have been identified in certain neoplasms. Also, terminology of various lesions in the gynaecological tract has changed and a couple of new entities have been described.
In this review we will highlight some mesenchymal neoplasms including gynaecological perivascular epithelioid cell tumour (PEComa), inflammatory myofibroblastic tumour (IMT) occurring in the uterus and discuss and review terminology of endometrial stromal neoplasms.
A recently...
Source: Pathology - July 12, 2015 Category: Pathology Tags: Reviews Source Type: research
Virological diagnosis of Ebolavirus infection
This article addresses the current situation and the ongoing challenges associated with delivering timely, high quality and safe testing within Australia for people exposed as part of the current major outbreak of Ebolavirus disease (EVD) in Western Africa. The members of the Public Health Laboratory Network have developed deliverable and reliable nucleic acid detection tests, and also have the laboratory capacity to handle the live viruses if necessary. However delivering and maintaining these services necessitates high levels of experience in developing and applying tests for exotic and emerging infections, strong nation...
Source: Pathology - July 12, 2015 Category: Pathology Tags: Special Theme Section: Laboratory Preparedness for Ebolavirus Disease: Guest Editor: Sharon C-A. Chen Source Type: research
Evaluation of point-of-care testing in critically unwell patients: comparison with clinical laboratory analysers and applicability to patients with Ebolavirus infection
Summary: Data on the performance of point-of-care (POC) or near-patient devices in the management of critically unwell patients are limited, meaning that there are demands for confirming POC test results in the routine clinical laboratory and so potentially leading to delay in treatment provision.
We evaluated the performance of the i-STAT CHEM 8+ and CG4+, Hemochron Signature Elite, HemoCue Hb 201+ and WBC Diff Systems on whole blood collected from medical and surgical patients admitted to the intensive care unit at an Australian tertiary care hospital. Measurements obtained for haematology, coagulation, biochemistry and ...
Source: Pathology - July 12, 2015 Category: Pathology Tags: Special Theme Section: Laboratory Preparedness for Ebolavirus Disease: Guest Editor: Sharon C-A. Chen Source Type: research
Preparing for Ebolavirus disease: specimen collection, packaging and transport
Summary: Ebolavirus is classified by Standards Australia as a Risk Group 4 pathogen for handling in laboratories. Specimens known or reasonably expected to contain Ebolavirus are classified by the United Nations as Dangerous Goods Infectious Substances Category A, UN 2814, which if transported by air must comply with International Air Transport Association (IATA) Hazard Class 6.2 and Packing Instruction 620 and Civil Aviation Safety Authority (CASA) Regulations. As such high risk pathogens are rarely encountered in pathology laboratories in Australia, the possibility of an imported case of Ebolavirus disease occurring in N...
Source: Pathology - July 12, 2015 Category: Pathology Tags: Special Theme Section: Laboratory Preparedness for Ebolavirus Disease: Guest Editor: Sharon C-A. Chen Source Type: research
Laboratory testing in management of patients with suspected Ebolavirus disease: infection control and safety
Summary: If routine laboratory safety precautions are followed, the risk of laboratory-acquired infection from handling specimens from patients with Ebolavirus disease (EVD) is very low, especially in the early ‘dry’ stage of disease. In Australia, border screening to identify travellers returning from EVD-affected west African countries during the 2014–2015 outbreak has made it unlikely that specimens from patients with unrecognised EVD would be sent to a routine diagnostic laboratory. Australian public health and diagnostic laboratories associated with hospitals designated for the care of patients with EVD have dev...
Source: Pathology - July 12, 2015 Category: Pathology Tags: Special Theme Section: Laboratory Preparedness for Ebolavirus Disease: Guest Editor: Sharon C-A. Chen Source Type: research
Editorial: Laboratory preparedness for Ebolavirus disease
No abstract available (Source: Pathology)
Source: Pathology - July 12, 2015 Category: Pathology Tags: Special Theme Section: Laboratory Preparedness for Ebolavirus Disease: Guest Editor: Sharon C-A. Chen Source Type: research
Koerbin G, Cavanaugh JA, Potter JM, Abhayaratna WP, West NP, Glasgow N, Hawkins C, Armbruster D, Oakman C, Hickman PE. ‘Aussie normals’: an a priori study to develop clinical chemistry reference intervals in a healthy Australian population. Pathology 2015; 47: 138–144.
No abstract available (Source: Pathology)
Source: Pathology - May 8, 2015 Category: Pathology Tags: Corrigendum Source Type: research
Re: Nationwide prevalence of lymph node metastases in Gleason score 3 + 3 = 6 prostate cancer: authors’ reply
No abstract available (Source: Pathology)
Source: Pathology - May 8, 2015 Category: Pathology Tags: Correspondence Source Type: research
Re: Nationwide prevalence of lymph node metastases in Gleason score 3 + 3 = 6 prostate cancer
No abstract available (Source: Pathology)
Source: Pathology - May 8, 2015 Category: Pathology Tags: Correspondence Source Type: research
Co-oximetry interference
No abstract available (Source: Pathology)
Source: Pathology - May 8, 2015 Category: Pathology Tags: Correspondence Source Type: research
Retrospective reassessment of gestational diabetes mellitus diagnosis by using the new classification
No abstract available (Source: Pathology)
Source: Pathology - May 8, 2015 Category: Pathology Tags: Correspondence Source Type: research
