Reassessment of H&E stained clot specimens and immunohistochemistry of phosphorylated Stat5 for histological diagnosis of MDS/MPN
Summary: Few studies have comprehensively analysed histopathological findings of bone marrow clots for diagnosis of haematopoietic cell dysplasia. In particular, a limited number of studies have assessed the use of haematoxylin and eosin (H&E) staining, which is generally considered less informative than May-Giemsa staining. In the current study, the utility of bone marrow clot specimens for diagnosis was examined using H&E staining and immunohistochemistry. Patients with myelodysplastic syndromes (MDS) and myelodysplastic/myeloproliferative neoplasm (MDS/MPN), including chronic myelomonocytic leukaemia (CMML), atypical ch...
Source: Pathology - December 1, 2015 Category: Pathology Tags: Haematology/Anatomical Pathology Source Type: research
Epithelioid rhabdomyosarcoma: a clinicopathological study of seven additional cases supporting a distinctive variant with aggressive biological behaviour
This study further demonstrates that epithelioid RMS represents a distinct variant of RMS with an aggressive behaviour. It may be misdiagnosed as poorly differentiated neuroendocrine carcinoma due to co-expression of epithelial and neuroendocrine markers. (Source: Pathology)
Source: Pathology - December 1, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Mammary analogue secretory carcinoma: an evaluation of its clinicopathological and genetic characteristics
Summary: Mammary analogue secretory carcinoma (MASC) is a recently described salivary gland malignancy. We evaluate the clinicopathological characteristics and long-term clinical behaviour of MASCs. A total of 190 primary salivary gland malignancies at a single institution were reviewed along with relevant immunohistochemical and fluorescent in situ hybridisation (FISH) studies to identify MASCs. Nine MASCs were identified predominantly in the parotid with an equal incidence in men and women and mean age of 36 years. The tumour size ranged from 14 to 50 mm (mean 22 mm). MASCs were composed of monotonous cells with vacu...
Source: Pathology - December 1, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Foamy gland changes in gastric-type endocervical neoplasia
This report expands the morphological spectrum exhibited by gastric-type endocervical lesions and the range of anatomical sites in which neoplasms with FG features may be encountered. (Source: Pathology)
Source: Pathology - December 1, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Clinicopathological characterisation of duodenal adenocarcinoma with high CD44 variant 9 expression
Summary: CD44 has been identified as a cancer stem cell (CSC) biomarker in various malignancies. The aim of this study was to elucidate the clinicopathological features of CD44v9 positive cells in duodenal adenocarcinoma (DA).
Twenty-nine DA patients were selected from medical archives at our hospital. CD44v9 expression was analysed together with that of MUC2, MUC5AC, and MUC6 by immunohistochemistry. High levels of CD44v9 expression weakly correlated with inflammatory cell infiltration (r = 0.431, p = 0.020) and MUC6 expression (r = 0.425, p = 0.022). Furthermore, double immunofluorescence staining of CD4...
Source: Pathology - December 1, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Concordance of HER2 expression in paired primary and metastatic sites of gastric and gastro-oesophageal junction cancers
Summary: HER2 is amplified/overexpressed in a subset of gastric and gastro-oesophageal junction cancers. Addition of anti-HER2 therapy has been shown to provide survival benefit in this setting. However, there are limited data assessing the concordance of HER2 status between primary and metastatic sites.
A total of 113 samples from 43 paired primary and metastatic tumours were tested for HER2 status, by immunohistochemistry (IHC) for protein expression and silver in situ hybridisation (SISH) for gene amplification.
Primary sites tested included endoscopic biopsies (n = 30) and resections (n = 24). Metastatic samp...
Source: Pathology - December 1, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Predicting discordant HER2 results in ipsilateral synchronous invasive breast carcinomas: experience from a single institution
Summary: With the emergence of multiple lines of highly effective Human Epidermal Growth Factor Receptor 2 (HER2) directed therapy, accurate identification of HER2 positive tumour has become a critical aspect in the histopathological analysis of breast cancers. Multifocal invasive breast carcinomas are relatively common, and given the aggressive inherent biology of HER2 positive disease, identification of even small tumours with HER2 positive status may be of importance for treatment planning. There are currently no clear guidelines as to whether all of these foci should be tested for HER2 status. We reviewed the results o...
Source: Pathology - December 1, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Prognostic relevance of proliferation markers (Ki-67, PHH3) within the cross-relation of ERG translocation and androgen receptor expression in prostate cancer
Summary: We evaluated the prognostic value of the mitosis-associated marker phosphorylated histone H3 (PHH3) and Ki-67 in prostate cancer with respect to ERG status and androgen receptor (AR) expression.
PHH3 and Ki-67 expression was immunohistochemically detected and digitally quantitated in a radical prostatectomy cohort (n = 640). The results were correlated to clinicopathological parameters including biochemical recurrence times. Prognostic values of PHH3 and Ki-67 were analysed by Cox regression and Kaplan–Meier statistics.
In prostate cancer, mean Ki-67 and PHH3 rates were 3.40% (95%CI 3.16–3.63%) and 0.015...
Source: Pathology - December 1, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Cancer stem cell markers in prostate cancer: an immunohistochemical study of ALDH1, SOX2 and EZH2
Summary: The aims of this study were to investigate the immunohistochemical expression and potential prognostic significance of putative cancer stems cell markers ALDH1, EZH2 and SOX2 in prostate cancer.
A total of 142 consecutive radical prostatectomies submitted to one laboratory with a diagnosis of prostatic adenocarcinoma between 2008 and 2012 were retrieved and retrospectively studied. Immunohistochemistry for the three markers was performed in each case and both univariate and multivariate analyses were undertaken to evaluate the correlation between the staining patterns and known histopathological prognostic featur...
Source: Pathology - December 1, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
The role of multiparametric flow cytometry in the detection of minimal residual disease in acute leukaemia
Summary: Flow cytometry is the most accessible method for minimal residual disease (MRD) detection due to its availability in most haematological centres. Using a precise combination of different antibodies, immunophenotypic detection of MRD in acute leukaemia can be performed by identifying abnormal combinations or expressions of antigens on malignant cells at diagnosis, during and post treatment. These abnormal phenotypes, referred to as leukaemia-associated immunophenotypes (LAIPs) are either absent or expressed at low frequency in normal bone marrow (BM) cells and are used to monitor the behaviour and quantitate the am...
Source: Pathology - December 1, 2015 Category: Pathology Tags: Review Source Type: research
Acknowledgement to reviewers
No abstract available (Source: Pathology)
Source: Pathology - November 19, 2015 Category: Pathology Tags: Acknowledgement to Reviewers Source Type: research
Amoebic encephalitis in a farmer
No abstract available (Source: Pathology)
Source: Pathology - November 19, 2015 Category: Pathology Tags: Correspondence Source Type: research
Evaluation of the Dual Path Platform syphilis point of care test in North Queensland
No abstract available (Source: Pathology)
Source: Pathology - November 19, 2015 Category: Pathology Tags: Correspondence Source Type: research
Evaluation of Abbott Architect high-sensitivity troponin I assay for haemolysis interference
No abstract available (Source: Pathology)
Source: Pathology - November 19, 2015 Category: Pathology Tags: Correspondence Source Type: research
Transfusions, troubled breathing, and transient neutropenia
No abstract available (Source: Pathology)
Source: Pathology - November 19, 2015 Category: Pathology Tags: Correspondence Source Type: research
