Concordance of HER2 expression in paired primary and metastatic sites of gastric and gastro-oesophageal junction cancers
Summary: HER2 is amplified/overexpressed in a subset of gastric and gastro-oesophageal junction cancers. Addition of anti-HER2 therapy has been shown to provide survival benefit in this setting. However, there are limited data assessing the concordance of HER2 status between primary and metastatic sites.
A total of 113 samples from 43 paired primary and metastatic tumours were tested for HER2 status, by immunohistochemistry (IHC) for protein expression and silver in situ hybridisation (SISH) for gene amplification.
Primary sites tested included endoscopic biopsies (n = 30) and resections (n = 24). Metastatic sample...
Source: Pathology - November 19, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Predicting discordant HER2 results in ipsilateral synchronous invasive breast carcinomas: experience from a single institution
Summary: With the emergence of multiple lines of highly effective Human Epidermal Growth Factor Receptor 2 (HER2) directed therapy, accurate identification of HER2 positive tumour has become a critical aspect in the histopathological analysis of breast cancers. Multifocal invasive breast carcinomas are relatively common, and given the aggressive inherent biology of HER2 positive disease, identification of even small tumours with HER2 positive status may be of importance for treatment planning. There are currently no clear guidelines as to whether all of these foci should be tested for HER2 status. We reviewed the results o...
Source: Pathology - November 19, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Prognostic relevance of proliferation markers (Ki-67, PHH3) within the cross-relation of ERG translocation and androgen receptor expression in prostate cancer
Summary: We evaluated the prognostic value of the mitosis-associated marker phosphorylated histone H3 (PHH3) and Ki-67 in prostate cancer with respect to ERG status and androgen receptor (AR) expression.
PHH3 and Ki-67 expression was immunohistochemically detected and digitally quantitated in a radical prostatectomy cohort (n = 640). The results were correlated to clinicopathological parameters including biochemical recurrence times. Prognostic values of PHH3 and Ki-67 were analysed by Cox regression and Kaplan–Meier statistics.
In prostate cancer, mean Ki-67 and PHH3 rates were 3.40% (95%CI 3.16–3.63%) and 0.0152%...
Source: Pathology - November 19, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Cancer stem cell markers in prostate cancer: an immunohistochemical study of ALDH1, SOX2 and EZH2
Summary: The aims of this study were to investigate the immunohistochemical expression and potential prognostic significance of putative cancer stems cell markers ALDH1, EZH2 and SOX2 in prostate cancer.
A total of 142 consecutive radical prostatectomies submitted to one laboratory with a diagnosis of prostatic adenocarcinoma between 2008 and 2012 were retrieved and retrospectively studied. Immunohistochemistry for the three markers was performed in each case and both univariate and multivariate analyses were undertaken to evaluate the correlation between the staining patterns and known histopathological prognostic feature...
Source: Pathology - November 19, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
The role of multiparametric flow cytometry in the detection of minimal residual disease in acute leukaemia
Summary: Flow cytometry is the most accessible method for minimal residual disease (MRD) detection due to its availability in most haematological centres. Using a precise combination of different antibodies, immunophenotypic detection of MRD in acute leukaemia can be performed by identifying abnormal combinations or expressions of antigens on malignant cells at diagnosis, during and post treatment. These abnormal phenotypes, referred to as leukaemia-associated immunophenotypes (LAIPs) are either absent or expressed at low frequency in normal bone marrow (BM) cells and are used to monitor the behaviour and quantitate the am...
Source: Pathology - November 19, 2015 Category: Pathology Tags: Correspondence Source Type: research
Prevalence of Arcobacter butzleri in the stool samples received in a tertiary hospital in Singapore: is it necessary to screen for it?
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
Human case of Balantidium infection in Australia
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
Judicious application of controls as a cost-effective approach to routine diagnostic PCR testing
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
Spiral bodies in pulse granulomas are remnant plant vascular structures
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
Preferential occurrence of RHOA mutation in gastric and colorectal cancers
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
Malignant peripheral nerve sheath tumour arising de novo from ganglioneuroma
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
Cutaneous ciliated cyst of the scrotum
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
BRAFV600E mutation is rare in clear cell ovarian carcinoma, but could potentially be identified with mutation specific immunohistochemistry
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
Aggressive MLL gene rearranged CD7+ CD56+ myeloid / natural killer cell precursor acute leukaemia with extramedullary relapse in the uterus and breast
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
Extensive bleeding due to an inhibitor in a haemophilia A patient with a Tyr2105Cys mutation: elimination of the inhibitor with rituximab
No abstract available (Source: Pathology)
Source: Pathology - September 11, 2015 Category: Pathology Tags: Correspondence Source Type: research
