Use of immunohistochemistry for SSTR2A to support a diagnosis of phosphaturic mesenchymal tumour
No abstract available (Source: Pathology)
Source: Pathology - January 8, 2015 Category: Pathology Tags: Correspondence Source Type: research
Purpuric exanthem caused by Ross River virus infection
No abstract available (Source: Pathology)
Source: Pathology - January 8, 2015 Category: Pathology Tags: Correspondence Source Type: research
Intraductal tubulopapillary neoplasm of pancreas with associated invasive carcinoma, lymph node, rectal and hepatic metastases
No abstract available (Source: Pathology)
Source: Pathology - January 8, 2015 Category: Pathology Tags: Correspondence Source Type: research
Evaluation of the BD Max Cdiff assay for the detection of toxigenic Clostridium difficile in human stool specimens
This study aimed to investigate the sensitivity and specificity of BD Max Cdiff assay for the detection of toxigenic C. difficile in an Australian setting. Between December 2013 and January 2014, 406 stool specimens from 349 patients were analysed with the BD Max Cdiff assay. Direct and enrichment toxigenic culture were performed on bioMérieux ChromID C. difficile agar as a reference method. isolates from specimens with discrepant results were further analysed with an in-house PCR to detect the presence of toxin genes. The overall prevalence of toxigenic C. difficile was 7.2%. Concordance between the BD Max assay and enri...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Microbiology Source Type: research
Extended spectrum beta-lactamase testing of community Enterobacteriaceae in the west of Australia: poor performance of phenotypic methods
SummaryExpanded spectrum β-lactamase (ESBL) producing organisms have long been recognised in institutions worldwide. Recently, community isolates producing ESBL have been reported overseas and in eastern Australia. We tested 571 consecutive Enterobacteriaceae urinary isolates from Western Australia and the Northern Territory phenotypically by calibrated dichotomous sensitivity (CDS) methods in two periods (2007 and 2012). Eleven ESBL-producing isolates from 2012 and 39 banked strains were genotyped by PCR. Twenty-six (4.6%) strains produced ESBL by CDS. Only 57.7% of CDS-confirmed ESBL strains had an initial reduced cepha...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Microbiology Source Type: research
Molecular surveillance for carbapenemase genes in carbapenem-resistant Pseudomonas aeruginosa in Australian patients with cystic fibrosis
SummaryThe aim of this study was to assess the prevalence of acquired carbapenemase genes amongst carbapenem non-susceptible Pseudomonas aeruginosa isolates in Australian patients with cystic fibrosis (CF). Cross-sectional molecular surveillance for acquired carbapenemase genes was performed on CF P. aeruginosa isolates from two isolate banks comprising: (i) 662 carbapenem resistant P. aeruginosa isolates from 227 patients attending 10 geographically diverse Australian CF centres (2007–2009), and (ii) 519 P. aeruginosa isolates from a cohort of 173 adult patients attending one Queensland CF clinic in 2011. All 1189 P. ae...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Microbiology Source Type: research
Prevalence of maternal red cell alloimmunisation: a population study from Queensland, Australia
SummaryThe aim of this study was to determine the current prevalence of red cell antigen alloimmunisation in Australia. Blood group (ABO and RhD) and red cell antibody screen results of pregnant women who presented at public hospitals in Queensland between the period of January 2011 and June 2013 were evaluated retrospectively. Antibody prevalence in pregnancy was compared to other published studies. A total of 482 positive antibody screens from 66,354 samples (0.73%) were identified. The prevalence of antibodies was: anti-E 27.6%; anti-D 10.4%; anti-Kell 9.5%; anti-c 8.7%; anti-Duffy 3.1%, including Fya and Fyb; anti-MNS ...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Haematology Source Type: research
Identification of a novel splicing mutation in the fibrinogen gamma chain gene leading to dysfibrinogenaemia in a Chinese pedigree
In this study, we investigated the molecular basis of dysfibrinogenaemia in a Chinese pedigree. Functional fibrinogen of venous blood samples was measured by Clauss method, and the antigens of fibrinogen in plasma were measured by immunoturbidimetry assay. All the exons and exon-intron boundaries of the three fibrinogen genes were analysed by direct sequencing. Fibrinogen electrophoresis, fibrinogen clottability measurement, fibrin polymerisation measurement, and electron microscopy scanning were also used in this study. The proband showed prolonged thrombin time, prolonged reptilase time, reduced fibrinogen activity level...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Haematology Source Type: research
‘Aussie normals’: an a priori study to develop clinical chemistry reference intervals in a healthy Australian population
We report on our findings for 40 of these analytes and two calculated parameters performed on the Abbott ARCHITECTci8200/ci16200 analysers. Not all samples were analysed for all assays due to volume requirements or assay/instrument availability. Results with elevated interference indices and those deemed unsuitable after clinical evaluation were removed from the database. Reference intervals were partitioned based on the method of Harris and Boyd into three scenarios, combined gender, males and females and age and gender. We have performed a detailed reference interval study on a healthy Australian population considering t...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Chemical Pathology Source Type: research
Plasma cholesterol in adults with phenylketonuria
SummaryPhenylketonuria (PKU) is an autosomal recessive disorder of phenylalanine (Phe) catabolism resulting from a deficiency of l-phenylalanine hydroxylase (PAH). An association between hyperphenylalaninaemia (HPA) and hypocholesterolaemia has been reported in children. However, controversy exists as to whether this is due to the low protein diet or to a disruption to cholesterol biosynthesis inherent to those with PKU. We investigated the relationship between blood Phe and plasma cholesterol in 41 apparently healthy adults with PKU (26 female, 15 male, age 18–57 years, median age 26 years) attending a PKU outpatient cl...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Chemical Pathology Source Type: research
CD117 and CD43 are useful adjuncts in the distinction of adenoid cystic carcinoma from adenoid basal cell carcinoma
We examined the immunohistochemical staining patterns with CD117 and CD43 in ACCs and BCCs, including BCCs with an adenoid growth pattern, to determine whether a combination of these markers can assist in the differential diagnosis.Fifteen cases each of ACC and BCC, including seven BCCs with a partial or entirely adenoid growth pattern were immunohistochemically stained for CD117 and CD43. The stains were interpreted semi-quantitatively.Staining for CD43 and CD117 was significantly more common in ACC than in BCC. Forty percent of ACCs showed staining for CD43, while no cases of BCC were positive. CD117 was positive in all ...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Diagnostic performance of CD66c in lung adenocarcinoma-associated malignant pleural effusion: comparison with CEA, CA 19-9, and CYFRA 21-1
This study evaluated the diagnostic ability of CD66c in lung adenocarcinoma-associated malignant pleural effusions (LA-MPEs) and compared it with other known tumour markers. Forty-seven cases of LA-MPE and 52 cases of benign pleural effusions were collected. The levels of CD66c, CEA, CA 19-9, and CYFRA 21-1 were measured by enzyme immunoassay. The expression of CD66c, CEA, and CA 19-9 in cell blocks was measured by immunocytochemistry. CEA had the best diagnostic values, with a sensitivity of 87.2% and specificity of 92.3%. Both CD66c and CA 19-9 showed the highest specificity of 98.1%, with sensitivities of 63.8% and 55.3...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
NUT protein immunoreactivity in ovarian germ cell tumours
SummaryThe aim of this study was to investigate NUT (nuclear protein in the testis) expression in ovarian germ cell tumours (GCTs). Immunostaining for NUT protein was performed in 10 mature cystic teratomas and in 49 malignant ovarian GCTs including 15 pure dysgerminomas, six dysgerminomas associated with gonadoblastoma, nine yolk sac tumours, 12 immature teratomas, and seven mixed malignant tumours. Only nuclear staining was considered a positive finding although cytoplasmic staining was noted when present. Thirty-seven (76%) malignant GCTs were NUT positive but staining was usually of weak to moderate intensity and obser...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
p16 immunoreactivity in endometrial stromal cells: stromal p16 expression characterises but is not specific for endometrial polyps
In conclusion, stromal p16 immunoreactivity is characteristic of EPs and this may reflect the pathogenesis of polyp formation. Immunohistochemistry can help to distinguish polypoid and non-polypoid mucosa, particularly in small or disrupted biopsy specimens. However, stromal p16 expression is not completely specific since focal staining may be present in normal endometrium. (Source: Pathology)
Source: Pathology - January 8, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
Comparative analysis of Napsin A, alpha-methylacyl-coenzyme A racemase (AMACR, P504S), and hepatocyte nuclear factor 1 beta as diagnostic markers of ovarian clear cell carcinoma: an immunohistochemical study of 279 ovarian tumours
SummaryNapsin A and α-methylacyl-coenzyme A racemase (AMACR, P504S) have recently been described as being frequently expressed in clear cell carcinomas (CCC) of the gynecological tract. The present study was conducted to assess the test performance of these newer markers relative to the more traditional marker, hepatocyte nuclear factor 1β (HNF1β), in a large and histotypically diverse dataset. A total of 279 ovarian tumours in tissue microarrays were immunohistochemically assessed for the expression of Napsin A, AMACR and HNF1β. HNF1β, Napsin A and AMACR were expressed in 92%, 82% and 63% of 65 CCC, 7%, 1% and 1% of ...
Source: Pathology - January 8, 2015 Category: Pathology Tags: Anatomical Pathology Source Type: research
