A galactose‐specific sugar: phosphotransferase permease is prevalent in the non‐core genome of Streptococcus mutans
Summary
Three genes predicted to encode the A, B and C domains of a sugar : phosphotransferase system (PTS) permease specific for galactose\(EIIGal) were identified in the genomes of 35 of 57 recently sequenced isolates of Streptococcus mutans, the primary etiological agent of human dental caries. Mutants defective in the EIIGal complex were constructed in six of the isolates and showed markedly reduced growth rates on galactose‐based medium relative to the parental strains. An EIIGal‐deficient strain constructed using the invasive serotype f strain OMZ175 (OMZ/IIGal) expressed significantly lower PTS activity when g...
Source: Oral Microbiology and Immunology - February 20, 2013 Category: Microbiology Authors: L. Zeng, P. Xue, M.J. Stanhope, R.A. Burne Tags: Original Article Source Type: research
A galactose‐specific sugar : phosphotransferase permease is prevalent in the non‐core genome of Streptococcus mutans
Summary
Three genes predicted to encode the A, B and C domains of a sugar : phosphotransferase system (PTS) permease specific for galactose\(EIIGal) were identified in the genomes of 35 of 57 recently sequenced isolates of Streptococcus mutans, the primary etiological agent of human dental caries. Mutants defective in the EIIGal complex were constructed in six of the isolates and showed markedly reduced growth rates on galactose‐based medium relative to the parental strains. An EIIGal‐deficient strain constructed using the invasive serotype f strain OMZ175 (OMZ/IIGal) expressed significantly lower PTS activity when g...
Source: Oral Microbiology and Immunology - February 20, 2013 Category: Microbiology Authors: L. Zeng, P. Xue, M.J. Stanhope, R.A. Burne Tags: Original Article Source Type: research
Sequence divergence in the Treponema denticola FhbB protein and its impact on factor H binding
In this study, we conduct a comparative assessment of the sequence, properties, structure, and ligand binding kinetics of the FhbB proteins of strains 33521 and 35405. The biological outcome of the interaction of these strains with FH could differ significantly as 33521 lacks dentilisin activity. The data presented here offer insight into our understanding of the interactions of T. denticola with the host and its potential to influence disease progression.
© 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - February 1, 2013 Category: Microbiology Authors: Daniel P. Miller, John V. McDowell, DeLacy V. Rhodes, Anna Allard, Melissa Caimano, Jessica Bell, Richard T. Marconi Tags: Original Article Source Type: research
A galactose‐specific sugar:phosphotransferase permease is prevalent in the non‐core genome of Streptococcus mutans
Summary
Three genes predicted to encode the A, B and C domains of a sugar:phosphotransferase system (PTS) permease specific for galactose (EIIGal) were identified in the genomes of 35 of 57recently‐sequenced isolates of Streptococcus mutans, the primary etiological agent of human dental caries. Mutants defective in the EIIGal complex were constructed in 6 of the isolates and showed markedly reduced growth rates on galactose‐based medium relative to the parental strains. An EIIGal‐deficient strain constructed using the invasive serotype f strain OMZ175 (OMZ/IIGal)expressed significantly lower PTS activitywhen galactos...
Source: Oral Microbiology and Immunology - January 25, 2013 Category: Microbiology Authors: Lin Zeng, Peng Xue, Michael J. Stanhope, Robert A. Burne Tags: Original Article Source Type: research
Characteristics of Porphyromonas gingivalis lipopolysaccharide in co‐culture with Fusobacterium nucleatum
Summary
Porphyromonas gingivalis is associated with chronic periodontitis and forms multi‐species biofilms. They can communicate within species as well as with other species found in the subgingiva, which may induce changes in the growth ratio and virulence of periodontopathogens. The lipopolysaccharide (LPS) of P. gingivalis shows different virulence by growth condition. The purpose of this study was to investigate the characteristics of P. gingivalis LPS when co‐cultured with Fusobacterium nucleatum. After culture of P. gingivalis in the presence or absence of F. nucleatum, P. gingivalis LPS was extracted. THP...
Source: Oral Microbiology and Immunology - January 24, 2013 Category: Microbiology Authors: S.H. Lee, D.H. Baek Tags: Original Article Source Type: research
Fermentative 2‐carbon metabolism produces carcinogenic levels of acetaldehyde in Candida albicans
Summary
Acetaldehyde is a carcinogenic product of alcohol fermentation and metabolism in microbes associated with cancers of the upper digestive tract. In yeast acetaldehyde is a by‐product of the pyruvate bypass that converts pyruvate into acetyl‐CoA during fermentation.
The aims of our study were: i) to determine the levels of acetaldehyde produced by C. albicans in the presence of glucose in low oxygen tension in vitro; ii) to analyze the expression levels of genes involved in the pyruvate‐bypass and acetaldehyde production; iii) to analyze whether any correlations exist between acetaldehyde levels, Adh enzyme act...
Source: Oral Microbiology and Immunology - January 19, 2013 Category: Microbiology Authors: Emilia Marttila, Paul Bowyer, Dominique Sanglard, Johanna Uittamo, Pertti Kaihovaara, Mikko Salaspuro, Malcolm Richardson, Riina Rautemaa Tags: Original Article Source Type: research
Toll‐like receptor 2‐mediated modulation of growth and functions of regulatory T cells by oral streptococci
This study was designed to determine whether oral streptococci modulate the growth and functions of regulatory T cells. Heat‐killed cells of wild‐type strains of S. gordonii and S. mutans induced the TLR2‐mediated NF‐κB activation, but their lipoprotein‐deficient strains did not. Stimulation with these streptococci resulted in significant increase in the frequency of CD4+CD25+ Foxp3+ regulatory T cells in splenocytes derived from both TLR2+/+ and TLR2‐/‐ mice, but level of increase in TLR2+/+ splenocytes was stronger than that in TLR2‐/‐ splenocytes. Both strains of S. gordonii enhanced the proliferation...
Source: Oral Microbiology and Immunology - December 1, 2012 Category: Microbiology Authors: Ayumi Saeki, Taku Segawa, Tsugumi Abe, Masahiro Sugiyama, Takafumi Arimoto, Hiroshi Hara, Akira Hasebe, Makoto Ohtani, Naoho Tanizume, Manabu Ohuchi, Hideo Kataoka, Masamitsu Kawanami, Atsuro Yokoyama, Ken‐ichiro Shibata Tags: Original Article Source Type: research
Genetic diversity and toxic activity of Aggregatibacter actinomycetemcomitans isolates
Oral Microbiology and Immunology,Volume 24, Issue 6, Page 493-501, December 2009. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - October 12, 2009 Category: Microbiology Source Type: research
Characterization of L ‐cysteine desulfhydrase from Prevotella intermedia
Oral Microbiology and Immunology,Volume 24, Issue 6, Page 485-492, December 2009. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - October 12, 2009 Category: Microbiology Source Type: research
Differential virulence and innate immune interactions of type I and II fimbrial genotypes of Porphyromonas gingivalis
Oral Microbiology and Immunology,Volume 24, Issue 6, Page 478-484, December 2009. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - October 12, 2009 Category: Microbiology Source Type: research
Progression of chronic periodontitis can be predicted by the levels of Porphyromonas gingivalis and Treponema denticola in subgingival plaque
Oral Microbiology and Immunology,Volume 24, Issue 6, Page 469-477, December 2009. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - October 12, 2009 Category: Microbiology Source Type: research
Influence of mouse prolactin ‐inducible protein in saliva on the aggregation of oral bacteria
Oral Microbiology and Immunology,Volume 24, Issue 6, Page 510-513, December 2009. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - October 12, 2009 Category: Microbiology Source Type: research
Detection of herpetic viruses in gingival crevicular fluid of patients suffering from periodontal diseases: prevalence and effect of treatment
Oral Microbiology and Immunology,Volume 24, Issue 6, Page 506-509, December 2009. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - October 12, 2009 Category: Microbiology Source Type: research
Helicobacter pylori in the oral cavity is associated with gastroesophageal disease
Oral Microbiology and Immunology,Volume 24, Issue 6, Page 464-468, December 2009. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - October 12, 2009 Category: Microbiology Source Type: research
Distribution and hydrolytic enzyme characteristics of Candida albicans strains isolated from diabetic patients and their non ‐diabetic consorts
Oral Microbiology and Immunology,Volume 24, Issue 6, Page 437-450, December 2009. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - October 12, 2009 Category: Microbiology Source Type: research
