Erythritol alters microstructure and metabolomic profiles of biofilm composed of Streptococcus gordonii and Porphyromonas gingivalis
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - June 22, 2013 Category: Microbiology Authors: Ei Hashino, Masae Kuboniwa, Samar A. Alghamdi, Mikiyo Yamaguchi, Reiko Yamamoto, Hiroshi Cho, Atsuo Amano Tags: Original Article Source Type: research
Involvement of lipoprotein PpiA of Streptococcus gordonii in evasion of phagocytosis by macrophages
In this study, we identified a putative lipoprotein, peptidyl‐prolyl cis/trans isomerase (PpiA), and clarified its role in evasion of phagocytosis by macrophages. Attenuation of the gene encoding prolipoprotein diacylglyceryl transferase (Lgt) altered the localization of PpiA from the cell surface to the culture supernatant, indicating that PpiA is lipid‐anchored in the cell membrane by Lgt. Both human and murine macrophages showed higher phagocytic activity towards ppiA and lgt mutants than the wild‐type, indicating that the presence of PpiA suppresses phagocytosis of S. gordonii. Human macrophages treated with dex...
Source: Oral Microbiology and Immunology - June 4, 2013 Category: Microbiology Authors: K. Cho, T. Arimoto, T. Igarashi, M. Yamamoto Tags: Original Article Source Type: research
Effects of short‐chain fatty acids on Actinomyces naeslundii biofilm formation
Summary
Actinomyces naeslundii is an early colonizer and has important roles in the development of the oral biofilm. Short‐chain fatty acids (SCFA) are secreted extracellularly as a product of metabolism by gram‐negative anaerobes, e.g. Porphyromonas gingivalis and Fusobacterium nucleatum; and the SCFA may affect biofilm development with interaction between A. naeslundii and gram‐negative bacteria. Our aim was to investigate the effects of SCFA on biofilm formation by A. naeslundii and to determine the mechanism. We used the biofilm formation assay in 96‐well microtiter plates in tryptic soy broth without dextros...
Source: Oral Microbiology and Immunology - June 3, 2013 Category: Microbiology Authors: S. Yoneda, T. Kawarai, N. Narisawa, E.B. Tuna, N. Sato, T. Tsugane, Y. Saeki, K. Ochiai, H. Senpuku Tags: Original Article Source Type: research
Oral Fusobacterium nucleatum subsp. polymorphum binds to human salivary α‐amylase
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - June 1, 2013 Category: Microbiology Authors: Maria Zulfiqar, Taihei Yamaguchi, Setsuko Sato, Takahiko Oho Tags: Original Article Source Type: research
MyD88 is essential for alveolar bone loss induced by Aggregatibacter actinomycetemcomitans LPS in mice
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - June 1, 2013 Category: Microbiology Authors: M. F. M. Madeira, C.M. Queiroz‐Junior, D. Cisalpino, S. M. C. Werneck, H. Kikuchi, O. Fujise, B. Ryffel, T. A. Silva, M. M. Teixeira, D. G. Souza Tags: Original Article Source Type: research
Perturbation of the indigenous rat oral microbiome by ciprofloxacin dosing
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - June 1, 2013 Category: Microbiology Authors: Pilar Manrique, Marcelo O. Freire, Casey Chen, Homa Zadeh, Mark Young, Peter Suci Tags: Original Article Source Type: research
Genetic and antigenic analyses of Porphyromonas gingivalis FimA fimbriae
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - May 31, 2013 Category: Microbiology Authors: Keiji Nagano, Yuki Abiko, Yasuo Yoshida, Fuminobu Yoshimura Tags: Original Article Source Type: research
Gingipain‐dependent degradation of mammalian target of rapamycin pathway proteins by the periodontal pathogen Porphyromonas gingivalis during invasion
In this study, we set out to investigate whether P. gingivalis and T. forsythia directly target key signalling molecules that may modulate the host cell phenotype to favour invasion and persistence. Our data identify, for the first time, that P. gingivalis, but not T. forsythia, reduces levels of intracellular mammalian target of rapamycin (mTOR) in oral epithelial cells following invasion over a 4‐h time course, via the action of gingipains. The ability of cytochalasin D to abrogate P. gingivalis‐mediated mTOR degradation suggests that this effect is dependent upon cellular invasion. We also show that levels of s...
Source: Oral Microbiology and Immunology - May 29, 2013 Category: Microbiology Authors: P. Stafford, J. Higham, A. Pinnock, C. Murdoch, C.W.I. Douglas, G.P. Stafford, D.W. Lambert Tags: Original Article Source Type: research
Membrane association and destabilization by Aggregatibacter actinomycetemcomitans leukotoxin requires changes in secondary structures
In this study, we tested this hypothesis by analysing lipid‐induced changes in leukotoxin conformation. Upon incubation of leukotoxin with lipids that favor leukotoxin‐membrane association, we observed an increase in leukotoxin α‐helical content that was not observed with lipids that favor membrane destabilization. The change in leukotoxin conformation after incubation with these lipids suggests that membrane binding and membrane destabilization have distinct secondary structural requirements, suggesting that they are independent events. These studies provide insight into the mechanism of cell damage that leads to d...
Source: Oral Microbiology and Immunology - May 16, 2013 Category: Microbiology Authors: M.J. Walters, A.C. Brown, T.C. Edrington, S. Baranwal, Y. Du, E.T. Lally, K. Boesze‐Battaglia Tags: Original Article Source Type: research
Involvement of lipoprotein PpiA of Streptococcus gordonii in evasion of phagocytosis by macrophages
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - May 4, 2013 Category: Microbiology Authors: Kasei Cho, Takafumi Arimoto, Takeshi Igarashi, Matsuo Yamamoto Tags: Original Article Source Type: research
Gingipain‐dependent degradation of mTOR pathway proteins by the periodontal pathogen Porphyromonas gingivalis during invasion
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - April 25, 2013 Category: Microbiology Authors: Prachi Stafford, Jon Higham, Abigail Pinnock, Craig Murdoch, C. W. Ian Douglas, Graham P Stafford, Daniel W Lambert Tags: Original Article Source Type: research
Effects of short‐chain fatty acids on Actinomyces naeslundii biofilm formation
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - April 20, 2013 Category: Microbiology Authors: Saori Yoneda, Taketo Kawarai, Naoki Narisawa, Elif Bahar Tuna, Norito Sato, Takanori Tsugane, Yoji Saeki, Kuniyasu Ochiai, Hidenobu Senpuku Tags: Original Article Source Type: research
Identification of β‐haemolysin‐encoding genes in Streptococcus anginosus
Summary
Streptococcus anginosus is an emerging pathogen, but little is known about its virulence factors. To detect the genes responsible for β‐haemolysis we performed genomic mutagenesis of the β‐haemolytic S. anginosus type strain ATCC 12395 using the vector pGhost9:ISS1. Integration site analysis of 15 non‐haemolytic mutants identified a gene cluster with high homology to the genes of the streptolysin S (SLS) encoding sag gene cluster of S. pyogenes. The gene cluster harbours 10 open reading frames displaying significant similarities to the S. pyogenes genes sagA‐sagI, with the identities on protein level r...
Source: Oral Microbiology and Immunology - April 18, 2013 Category: Microbiology Authors: D. Asam, S. Mauerer, E. Walheim, B. Spellerberg Tags: Original Article Source Type: research
Membrane Association and Destabilization by Aggregatibacter actinomycetemcomitans Leukotoxin Requires Changes in Secondary Structures
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - April 11, 2013 Category: Microbiology Authors: Michael J. Walters, Angela C. Brown, Thomas C. Edrington, Somesh Baranwal, Yurong Du, Edward T. Lally, Kathleen Boesze‐Battaglia Tags: Original Article Source Type: research
Identification of β‐haemolysin encoding genes in Streptococcus anginosus
Summary
Streptococcus anginosus is an emerging pathogen, but little is known about its virulence factors. To detect the genes responsible for ß‐haemolysis we performed genomic mutagenesis of the ß‐hemolytic S. anginosus type strain ATCC 12395 using the vector pGhost9:ISS1. Integration site analysis of 15 non‐haemolytic mutants identified a gene cluster with high homology to the genes of the streptolysin S (SLS) encoding sag gene cluster of S. pyogenes. The gene cluster harbours 10 open reading frames displaying significant similarities to the S. pyogenes genes sagA‐sagI, with the identities between the different ...
Source: Oral Microbiology and Immunology - February 28, 2013 Category: Microbiology Authors: Daniela Asam, Stefanie Mauerer, Ellen Walheim, Barbara Spellerberg Tags: Original Article Source Type: research
