Cnm is a major virulence factor of invasive Streptococcus mutans and part of a conserved three‐gene locus
Summary
Cnm, a collagen‐ and laminin‐binding protein present in a subset of Streptococcus mutans strains, mediates binding to extracellular matrices (ECM), intracellular invasion and virulence in the Galleria mellonella model. Antibodies raised against Cnm were used to confirm expression and the cell surface localization of Cnm in the highly invasive OMZ175 strain. Sequence analysis identified two additional genes (cnaB and cbpA) encoding putative surface proteins immediately upstream of cnm. Inactivation of cnaB and cbpA in OMZ175, individually or in combination, did not decrease the ability of this highly invasive an...
Source: Oral Microbiology and Immunology - February 12, 2014 Category: Microbiology Authors: A. Avilés‐Reyes, J.H. Miller, P.J. Simpson‐Haidaris, J.A. Lemos, J. Abranches Tags: Original Article Source Type: research
Danger signal adenosine via adenosine 2a receptor stimulates growth of Porphyromonas gingivalis in primary gingival epithelial cells
This study reveals that the GEC express functional A2a receptor and P. gingivalis may use the A2a receptor coupled DS adenosine signaling as a means to establish successful persistence in the oral mucosa, possibly via downregulation of the pro‐inflammatory response. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - February 12, 2014 Category: Microbiology Authors: R. Spooner, J. DeGuzman, K.L. Lee, Ö. Yilmaz Tags: Original Article Source Type: research
Adherence of Candida albicans to silicone is promoted by the human salivary protein SPLUNC2/PSP/BPIFA2
Summary
Interactions between Candida albicans, saliva and saliva‐coated oral surfaces are initial events in the colonization of the oral cavity by this commensal yeast, which can cause oral diseases such as candidiasis and denture stomatitis. Candida albicans also colonizes silicone voice prostheses, and the microbial biofilm formed can impair valve function, necessitating frequent prosthesis replacement. We have previously shown that saliva promoted binding of C. albicans cells to silicone in vitro, and that the selective binding of specific salivary proteins to voice prosthesis silicone mediated attachment of C. albi...
Source: Oral Microbiology and Immunology - February 8, 2014 Category: Microbiology Authors: A.R. Holmes, E. Rodrigues, P. Wielen, K.M. Lyons, B.J. Haigh, T.T. Wheeler, P.J.D. Dawes, R.D. Cannon Tags: Original Article Source Type: research
Looking in the Porphyromonas gingivalis cabinet of curiosities: the microbium, the host and cancer association
Summary
The past decades of biomedical research have yielded massive evidence for the contribution of the microbiome in the development of a variety of chronic human diseases. There is emerging evidence that Porphyromonas gingivalis, a well‐adapted opportunistic pathogen of the oral mucosa and prominent constituent of oral biofilms, best known for its involvement in periodontitis, may be an important mediator in the development of a number of multifactorial and seemingly unrelated chronic diseases, such as rheumatoid arthritis and orodigestive cancers. Orodigestive cancers represent a large proportion of the total malign...
Source: Oral Microbiology and Immunology - February 8, 2014 Category: Microbiology Authors: K.R. Atanasova, Ö. Yilmaz Tags: Invited Review Source Type: research
Innocent until proven guilty: mechanisms and roles of Streptococcus‐Candida interactions in oral health and disease
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - February 1, 2014 Category: Microbiology Authors: Hongbin Xu, Howard F. Jenkinson, Anna Dongari‐Bagtzoglou Tags: Review Article Source Type: research
The pga gene cluster in Aggregatibacter actinomycetemcomitans is necessary for the development of natural competence in Ca2+‐promoted biofilms
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - January 23, 2014 Category: Microbiology Authors: Kyoko Hisano, Osamu Fujise, Mayumi Miura, Takafumi Hamachi, Etsuko Matsuzaki, Fusanori Nishimura Tags: Original Article Source Type: research
Danger signal adenosine via adenosine 2a receptor stimulates growth of Porphyromonas gingivalis in primary gingival epithelial cells
This study reveals that the GEC express functional A2a receptor and P. gingivalis may use the A2a receptor coupled DS adenosine signaling as a means to establish successful persistence in the oral mucosa, possibly via downregulation of the pro‐inflammatory response. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - January 23, 2014 Category: Microbiology Authors: R. Spooner, J. DeGuzman, K.L. Lee, Ö. Yilmaz Tags: Original Article Source Type: research
Looking in the Porphyromonas gingivalis’ Cabinet of Curiosities: The Microbium, the Host and Cancer Association
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - January 22, 2014 Category: Microbiology Authors: Kalina R Atanasova, Özlem Yilmaz Tags: Invited Review Source Type: research
Adherence of Candida albicans to silicone is promoted by the human salivary protein SPLUNC2/PSP/BPIFA2
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - January 19, 2014 Category: Microbiology Authors: Ann R. Holmes, Ely Rodrigues, Pauline Weilen, Karl M. Lyons, Brendan Haigh, Thomas T. Wheeler, Patrick J.D. Dawes, Richard D. Cannon Tags: Original Article Source Type: research
Genetic basis of coaggregation receptor polysaccharide biosynthesis in Streptococcus sanguinis and related species
Summary
Interbacterial adhesion between streptococci and actinomyces promotes early dental plaque biofilm development. Recognition of coaggregation receptor polysaccharides (RPS) on strains of Streptococcus sanguinis, Streptococcus gordonii and Streptococcus oralis by Actinomyces spp. type 2 fimbriae is the principal mechanism of these interactions. Previous studies of genetic loci for synthesis of RPS (rps) and RPS precursors (rml, galE1 and galE2) in S. gordonii 38 and S. oralis 34 revealed differences between these strains. To determine whether these differences are strain‐specific or species‐specific, we identifi...
Source: Oral Microbiology and Immunology - January 8, 2014 Category: Microbiology Authors: J. Yang, Y. Yoshida, J.O. Cisar Tags: Original Article Source Type: research
Porphyromonas gingivalis C‐terminal signal peptidase PG0026 and HagA interact with outer membrane protein PG27/LptO
Summary
Outer membrane protein PG27 is essential for secretion/maturation of conserved C‐terminal domain (CTD) proteins such as gingipains, HagA, and PG0026. To determine the binding partner(s) of PG27, we used a Porphyromonas gingivalis mutant strain, 83K48, which expressed functional histidine‐tagged PG27. Purification of histidine‐tagged PG27 from 83K48 found that 136‐kDa and 264‐kDa proteins accompanied histidine‐tagged PG27. Mass spectrometry revealed the 136‐kDa protein and 264‐kDa protein to be PG0026 and PG1837 (HagA), respectively. PG0026 is a C‐terminal signal peptidase which cleaves the CTDs of...
Source: Oral Microbiology and Immunology - December 2, 2013 Category: Microbiology Authors: K. Saiki, K. Konishi Tags: Original Article Source Type: research
The effect of arginine on oral biofilm communities
This study tested the hypothesis that increased availability of arginine in the oral environment through an exogenous source enhances the ADS activity levels in saliva and dental plaque. Saliva and supra‐gingival plaque samples were collected from 19 caries‐free (CF) individuals (DMFT = 0) and 19 caries‐active (CA) individuals (DMFT ≥ 2) before and after treatment, which comprised the use of a fluoride‐free toothpaste containing 1.5% arginine, or a regular fluoride‐containing toothpaste twice daily for 4 weeks. ADS activity was measured by quantification of ammonia produced from arginine by oral samples at...
Source: Oral Microbiology and Immunology - December 2, 2013 Category: Microbiology Authors: M.M. Nascimento, C. Browngardt, X. Xiaohui, V. Klepac‐Ceraj, B.J. Paster, R.A. Burne Tags: Original Article Source Type: research
Danger Signal Adenosine via Adenosine 2a Receptor Stimulates Growth of P. gingivalis in Primary Gingival Epithelial Cells
This study reveals that the GECs express functional A2a receptor and P. gingivalis may utilize the A2a receptor coupled DS Adenosine signaling as a means to establish successful persistence in the oral mucosa, possibly via down‐regulation of pro‐inflammatory response.
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - December 1, 2013 Category: Microbiology Authors: Ralee Spooner, Jefferson DeGuzman, KyuLim Lee, Özlem Yilmaz Tags: Original Article Source Type: research
The effect of arginine on oral biofilm communities
This study tested the hypothesis that increased availability of arginine in the oral environment through an exogenous source enhances the ADS activity levels in dental plaque and saliva. Saliva and supra‐gingival plaque samples were collected from 19 caries‐free (CF) individuals (DMFT=0) and 19 caries‐active (CA) individuals (DMFT ≥ 2) before and after treatment, which comprised the use of a fluoride‐free toothpaste containing 1.5% arginine, or a regular fluoride‐containing toothpaste twice daily for 4 weeks. ADS activity was measured by quantification of ammonia produced from arginine by oral samples at baseli...
Source: Oral Microbiology and Immunology - November 21, 2013 Category: Microbiology Authors: Marcelle M. Nascimento, Christopher Browngardt, Xu Xiaohui, Vanja Klepac‐Ceraj, Bruce J. Paster, Robert A. Burne Tags: Original Article Source Type: research
Porphyromonas gingivalis C‐terminal signal peptidase PG0026 and HagA interact with outer membrane protein PG27/LptO
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - November 15, 2013 Category: Microbiology Authors: Keitarou Saiki, Kiyoshi Konishi Tags: Original Article Source Type: research
