The role of phagocytosis, oxidative burst and neutrophil extracellular traps in the interaction between neutrophils and the periodontal pathogen Porphyromonas gingivalis
In conclusion, phagocytosis is the primary neutrophil response to P. gingivalis, although NETs could play an accessory role in infection control. Although gingipains do not seem to directly regulate phagocytosis, NETs or oxidative burst in neutrophils, their proteolytic properties could modulate the subsequent outcomes such as nutrition acquisition and survival by the bacteria. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - May 4, 2015 Category: Microbiology Authors: K. Jayaprakash, I. Demirel, H. Khalaf, T. Bengtsson Tags: Original Article Source Type: research
Candida–streptococcal mucosal biofilms display distinct structural and virulence characteristics depending on growth conditions and hyphal morphotypes
This study evaluated structural and virulence characteristics of Candida–streptococcal biofilms formed on moist or semidry mucosal surfaces, and tested the effects of nutrient availability and hyphal morphotype on dual‐species biofilms. Three‐dimensional models of the oral mucosa formed by immortalized keratinocytes on a fibroblast‐embedded collagenous matrix were used. Infections were carried out using Streptococcus oralis strain 34, in combination with a C. albicans wild‐type strain, or pseudohyphal‐forming mutant strains. Increased moisture promoted a homogeneous surface biofilm by C. albicans. Dual biofil...
Source: Oral Microbiology and Immunology - April 20, 2015 Category: Microbiology Authors: M.M. Bertolini, H. Xu, T. Sobue, C.J. Nobile, A. A. Del Bel Cury, A. Dongari‐Bagtzoglou Tags: Original Article Source Type: research
A. actinomycetemcomitans QseBC is activated by catecholamines and iron and regulates genes encoding proteins associated with anaerobic respiration and metabolism
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - April 1, 2015 Category: Microbiology Authors: Whitney A. Weigel, Donald R. Demuth, Ascención Torres‐Escobar, Maria Dolores Juárez‐Rodríguez Tags: Original Article Source Type: research
Live‐cell and super‐resolution imaging reveal that the distribution of wall‐associated protein A is correlated with the cell chain integrity of Streptococcus mutans
This study applied the fluorescence protein mCherry as a reporter gene to characterise the dynamic distribution of WapA in S. mutans via time lapse and super‐resolution fluorescence imaging. The results revealed interesting subcellular distribution patterns of WapA in single, dividing, and long chains of S. mutans cells. It appears at the middle of the cell and moves to the poles as the cell grows and divides. In a cell chain, after each round of cell division, such dynamic relocation results in WapA distribution at the previous cell division sites, resulting in a pattern where WapA is located at the boundary of two adja...
Source: Oral Microbiology and Immunology - April 1, 2015 Category: Microbiology Authors: Yongliang Li, Zhen Liu, Yifei Zhang, Qian P. Su, Boxin Xue, Shipeng Shao, Yun Zhu, Xiao Xu, Shicheng Wei, Yujie Sun Tags: Original Article Source Type: research
The Role of Phagocytosis, oxidative burst and NETs in the interaction between neutrophils and the periodontal pathogen Porphyromonas gingivalis
In conclusion, phagocytosis is the primary neutrophil response to P.gingivalis, although NETs could play an accessory role in infection control. Although gingipains do not seem to directly regulate phagocytosis, NETs or oxidative burst in neutrophils, its proteolytic properties could modulate the subsequent outcomes such as nutrition acquisition and survival by the bacteria.
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - April 1, 2015 Category: Microbiology Authors: Kartheyaene Jayaprakash, Isak Demirel, Hazem Khalaf, Torbjörn Bengtsson Tags: Original Article Source Type: research
The roles of RgpB and Kgp in late onset gingipain activity in the vimA‐defective mutant of Porphyromonas gingivalis W83
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Source: Oral Microbiology and Immunology - April 1, 2015 Category: Microbiology Authors: Yuetan Dou, Antonette Robles, Francis Roy, Wilson Aruni, Lawrence Sandberg, Eugene Nothnagel, Hansel M. Fletcher Tags: Original Article Source Type: research
Identification of Amino Acid Residues Involved in Hemin Binding in Porphyromonas gingivalis Hemagglutinin 2
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - April 1, 2015 Category: Microbiology Authors: Qiu Bo Yang, Fei Yan Yu, Lu Sun, Qi Xia Zhang, Mei Lin, XiaoYu Geng, Xiao ni Sun, Jin Lu Li, Ying Liu Tags: Original Article Source Type: research
Secretome of gingival epithelium in response to subgingival biofilms
In conclusion, the present study provides the secreted proteome profile of gingival epithelial tissue to subgingival biofilms, and identifies a significant role for the red complex species in the observed effects.
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - March 19, 2015 Category: Microbiology Authors: Nagihan Bostanci, Kai Bao, Asa Wahlander, Jonas Grosmann, Thomas Thurnheer, Georgios N. Belibasakis Tags: Original Article Source Type: research
TRIB3 mediates the expression of Wnt5a and activation of nuclear factor‐κB in Porphyromonas endodontalis lipopolysaccharide‐treated osteoblasts
Summary
Porphyromonas endodontalis lipopolysaccharide (LPS) is considered to be correlated with the progression of bone resorption in periodontal and periapical diseases. Wnt5a has recently been implicated in inflammatory processes, but its role is unclear as a P. endodontalisLPS‐induced mediator in osteoblasts. Tribbles homolog 3 (TRIB3) encodes a pseudokinase and has been linked to inflammation in certain situations. Here, we found that P. endodontalisLPS induced Wnt5a expression in a dose‐ and time‐dependent manner and it also upregulated translocation, phosphorylation and transcriptional activity of nuclear fac...
Source: Oral Microbiology and Immunology - March 9, 2015 Category: Microbiology Authors: Y. Yu, L. Qiu, J. Guo, D. Yang, L. Qu, J. Yu, F. Zhan, M. Xue, M. Zhong Tags: Original Article Source Type: research
Candida‐streptococcal mucosal biofilms display distinct structural and virulence characteristics depending on growth conditions and hyphal morphotypes
This study evaluated structural and virulence characteristics of Candida‐streptococcal biofilms formed on moist or semidry mucosal surfaces, and tested the effects of nutrient availability and hyphal morphotype on dual‐species biofilms. Three‐dimensional models of the oral mucosa formed by immortalized keratinocytes on a fibroblast‐embedded collagenous matrix were used. Infections were carried out using Streptococcus oralis strain 34, in combination with a C. albicans wild‐type strain, or pseudohyphal‐forming mutant strains. Increased moisture promoted a homogeneous surface biofilm by C. albicans. Dual biofilms...
Source: Oral Microbiology and Immunology - February 1, 2015 Category: Microbiology Authors: Martinna M. Bertolini, Hongbin Xu, Takanori Sobue, Clarissa J. Nobile, Altair Cury, Anna Dongari‐Bagtzoglou Tags: Original Article Source Type: research
TRIB3 mediates the expression of Wnt5a and activation of NF‐κB in Porphyromonas endodontalis lipopolysaccharide‐treated osteoblasts
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - January 20, 2015 Category: Microbiology Authors: Yaqiong Yu, Lihong Qiu, Jiajie Guo, Di Yang, Liu Qu, Jingtao Yu, Fuliang Zhan, Ming Xue, Ming Zhong Tags: Original Article Source Type: research
A YadA‐like autotransporter, Hag1 in Veillonella atypica is a multivalent hemagglutinin involved in adherence to oral streptococci, Porphyromonas gingivalis, and human oral buccal cells
In this study, we used our recently discovered transformable Veillonella strain, Veillonella atypica OK5, to probe the mechanism of coaggregation between Veillonella species and other oral bacteria. By insertional inactivation of all eight putative hemagglutinin genes, we identified one gene, hag1, which is involved in V. atypica coaggregation with the initial colonizers Streptococcus gordonii, Streptococcus oralis and Streptococcus cristatus, and the periodontal pathogen Porphyromonas gingivalis. The hag1 mutant also abolished adherence to human buccal cells. Inhibition assays using various chemical or physiological trea...
Source: Oral Microbiology and Immunology - January 19, 2015 Category: Microbiology Authors: P. Zhou, J. Liu, J. Merritt, F. Qi Tags: Original Article Source Type: research
Editorial
(Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - January 14, 2015 Category: Microbiology Authors: Richard J. Lamont Tags: Editorial Source Type: research
Porphyromonas gingivalis RagB is a proinflammatory signal transducer and activator of transcription 4 agonist
Summary
Periodontal diseases are semi‐ubiquitous and caused by chronic, plaque‐induced inflammation. The 55‐kDa immunodominant RagB outer membrane protein of Porphyromonas gingivalis, a keystone periodontal pathogen, has been proposed to facilitate nutrient transport. However, potential interactions between RagB and the innate response have not been examined. We determined that RagB exposure led to the differential and dose‐related expression of multiple genes encoding proinflammatory mediators [interleukin‐1α (IL‐1α), IL‐1β, IL‐6, IL‐8 and CCL2; all P < 0.05] in primary human monocytes and to the...
Source: Oral Microbiology and Immunology - January 11, 2015 Category: Microbiology Authors: J.A. Hutcherson, J. Bagaitkar, K. Nagano, F. Yoshimura, H. Wang, D.A. Scott Tags: Original Article Source Type: research
Regulation of Streptococcus mutans PTSBio by the Transcriptional Repressor NigR
This article is protected by copyright. All rights reserved. (Source: Oral Microbiology and Immunology)
Source: Oral Microbiology and Immunology - December 1, 2014 Category: Microbiology Authors: Milos Vujanac, Vijayalakshmi S. Iyer, Mrittika Sengupta, Dragana Ajdic Tags: Original Article Source Type: research
