TRIB3 mediates the expression of Wnt5a and activation of nuclear factor‐κB in Porphyromonas endodontalis lipopolysaccharide‐treated osteoblasts

Summary Porphyromonas endodontalis lipopolysaccharide (LPS) is considered to be correlated with the progression of bone resorption in periodontal and periapical diseases. Wnt5a has recently been implicated in inflammatory processes, but its role is unclear as a P. endodontalisLPS‐induced mediator in osteoblasts. Tribbles homolog 3 (TRIB3) encodes a pseudokinase and has been linked to inflammation in certain situations. Here, we found that P. endodontalisLPS induced Wnt5a expression in a dose‐ and time‐dependent manner and it also upregulated translocation, phosphorylation and transcriptional activity of nuclear factor‐κB (NF‐κB) in MC3T3‐E1 cells. Bay 11‐7082 blocked the translocation of NF‐κB and Wnt5a expression induced by P. endodontalisLPS. Chromatin immunoprecipitation assay further established that induction of Wnt5a by P. endodontalisLPS was mediated through the NF‐κB p65 subunit. Additionally, P. endodontalisLPS increased expression of TRIB3 in osteoblasts after 10 h simulated time. Overexpression of TRIB3 enhanced NF‐κB phosphorylation and Wnt5a induction, whereas knockdown of TRIB3 inhibited NF‐κB phosphorylation and Wnt5a expression in P. endodontalisLPS‐stimulated osteoblasts. These results suggest that P. endodontalisLPS has the ability to promote the expression of Wnt5a in mouse osteoblasts, and this induction is mainly mediated by NF‐κB pathway. TRIB3 seems to modulate the sustained expression of Wnt5a in osteoblasts sti...
Source: Oral Microbiology and Immunology - Category: Microbiology Authors: Tags: Original Article Source Type: research