Biological application of focus ion beam-scanning electron microscopy (FIB-SEM) to the imaging of cartilaginous fibrils and osteoblastic cytoplasmic processes
Conclusion Taken together, these data suggest that FIB-SEM imaging of serial bone sections may facilitate new insights on the ultrastructure of cartilage and bone tissues. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - December 4, 2016 Category: Biomedical Science Source Type: research
Fibroblasts and macrophages: Key players in the head and neck cancer microenvironment
Conclusion CAFs can promote HNSCC progression through direct contact and/or paracrine signaling. Interactions between HNSCC cells and CAFs stimulate expression of various growth factors, cytokines, chemokines, MMPs, and periostin. TAMs in HNSCC upregulate the production of IL-1β, IL-10, and MIP-3α/CCL20, which are involved in tumorigenic processes. Graphical abstract (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - November 24, 2016 Category: Biomedical Science Source Type: research
Porphyromonas gingivalis promotes low-density lipoprotein oxidation and atherosclerosis
Conclusions These results suggest that Pg promotes LDL oxidation and contributes to atheroma development. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - November 10, 2016 Category: Biomedical Science Source Type: research
Aneuploidy of a murine immortalized endothelial cell line, MS1
Conclusion MS1 cells are aneuploid and activate H2AX to a greater extent than NECs do. MS1 cells resemble TECs in terms of the proliferative phenotype and aneuploidy. Thus, MS1 may be a good cell line for studies on the relation between chromosomal instability and a DDR in nonmalignant cells. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - November 4, 2016 Category: Biomedical Science Source Type: research
The small GTPase, Rab27, and its effectors and regulators participate in granule exocytosis by parotid acinar cells
Conclusion Exocytosis occurs via a Rab27 GDP/GTP cycling mechanism in parotid acinar cells. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - November 3, 2016 Category: Biomedical Science Source Type: research
Evaluation of a new mouse model for studying dental pulpal responses to GaAlAs laser irradiation
Conclusions The current output energy induced apoptosis in the affected dental pulp, followed by active cell proliferation, resulting in tertiary dentin formation. This is the first report regarding laser irradiation of teeth in an in vivo mouse model. This model could enable further understanding of the function of certain proteins, including transcriptional factors. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - October 28, 2016 Category: Biomedical Science Source Type: research
Relationship between localization of proteoglycans and induction of neurotrophic factors in mouse dental pulp
Conclusions These data suggest that heparan sulfate proteoglycans, such as perlecan, enhance the expression of GDNF by facilitating FGF-induced Erk1/2 signaling in the subodontoblastic layer. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - October 15, 2016 Category: Biomedical Science Source Type: research
Unveiling a rhythmic regulatory mode hidden in developmental tissue growth by fluorescence live imaging-based mathematical modeling
Conclusion The scope of this review is to describe the experimental and theoretical evidence for cell cycle progression as a stochastic molecular and cellular process. In addition, we elucidate the rhythmic mode of tissue development that endows “biological robustness” for reproducible tissue development. Finally, we discuss the potential of the live imaging-based mathematical approach for understanding pathophysiological development in tissues. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - September 10, 2016 Category: Biomedical Science Source Type: research
Analysis of calcium, phosphorus, and carbon concentrations during developmental calcification of dentin and enamel in rat incisors using scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX)
Conclusions During tooth development, the initial dentin matrix may possess distinctive mineral characteristics as compared with other parts of dentin and predentin. Elemental composition of the mineral in enamel may change during late maturation. Our results are suggestive of degradation of organic components during developmental calcification in dentin and enamel. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - September 2, 2016 Category: Biomedical Science Source Type: research
Dentin sialophosphoprotein is a potentially latent bioactive protein in dentin
Conclusion The functions of DSPP for integrin-mediated signaling possibly arise from the proteolytic cleavage of the peptide bonds close to the RGD domain and induce reactionary dentinogenesis in vivo. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - August 26, 2016 Category: Biomedical Science Source Type: research
Cultivation strategies for growth of uncultivated bacteria
Conclusion Despite significant advances in recent years in methodologies for the cultivation of previously uncultivated bacteria, a substantial proportion remain to be cultured and efforts to devise high-throughput strategies should be a high priority. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - August 10, 2016 Category: Biomedical Science Source Type: research
Dentin sialophosphoprotein-derived proteins in porcine pulp and dentin – Gene expression and function
Conclusion DSP-only is expressed throughout odontoblast differentiation, while full-length DSPP is predominantly expressed by odontoblasts only after they have differentiated from mesenchymal cells. DPP and DSP rescued the loss of TGF-β1 activity. Type I collagen was infrequently bound to CF-hTGF-β1. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - August 5, 2016 Category: Biomedical Science Source Type: research
Proliferative activity of skeletal myoblast sheet by paracrine effects of mesenchymal stem cells
Conclusions This study demonstrated that the presence of MSCs could transiently enhance the proliferative activity of myoblasts, but that this enhancement is ultimately diminished due to contact inhibition. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - August 5, 2016 Category: Biomedical Science Source Type: research
New findings for dentin sialophosphoprotein studies: Applications of purified odontoblast-like cells derived from stem cells
Conclusion Mouse embryonic stem cells, mouse induced pluripotent stem cells, and α7+ human skeletal muscle stem cells can differentiate into odontoblast-like cells without an epithelial–mesenchymal transition, and are characterized by the signaling cascade underpinning odontoblastic differentiation, which may be useful for tooth regeneration and dentin sialophosphoprotein (DSPP) studies. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - August 5, 2016 Category: Biomedical Science Source Type: research
Degradation of bradykinin by a metalloendopeptidase from Streptococcus pyogenes
Conclusions S. pyogenes produces a cell-associated metalloendopeptidase that degrades bradykinin. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - August 5, 2016 Category: Biomedical Science Source Type: research
