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Mimicking the host and its microenvironment in vitro for studying mucosal infections by Pseudomonas aeruginosa
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - April 1, 2014 Category: Microbiology Authors: Aurélie Crabbé, Maria A. Ledesma, Cheryl A. Nickerson Tags: MiniReview Source Type: research

The role of viral persistence in flavivirus biology
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - April 1, 2014 Category: Microbiology Authors: Luwanika Mlera, Wessam Melik, Marshall E. Bloom Tags: MiniReview Source Type: research

The NIAID Integrated Research Facility at Frederick, Maryland: A unique international resource to facilitate medical countermeasure development for BSL‐4 pathogens
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - March 29, 2014 Category: Microbiology Authors: Peter B. Jahrling, Lauren Keith, Marisa St. Claire, Reed F. Johnson, Laura Bollinger, Matthew G. Lackemeyer, Lisa E. Hensley, Jason Kindrachuk, Jens H. Kuhn Tags: MiniReview Source Type: research

Genetic Control of Weight Loss During Pneumonic Burkholderia pseudomallei Infection
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - March 29, 2014 Category: Microbiology Authors: Felicia D. Emery, Jyothi Parvathareddy, Ashutosh K. Pandey, Yan Cui, Robert W. Williams, Mark A. Miller Tags: Research Paper Source Type: research

Issue Information
(Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - March 14, 2014 Category: Microbiology Tags: Issue Information Source Type: research

Proteomic strategies for the discovery of novel diagnostic and therapeutic targets for infectious diseases
Abstract Viruses have developed numerous and elegant strategies to manipulate the host cell machinery to establish a productive infectious cycle. The interaction of viral proteins with host proteins plays an important role in infection and pathogenesis, often bypassing traditional host defenses such as the interferon response and apoptosis. Host–viral protein interactions can be studied using a variety of proteomic approaches ranging from genetic and biochemical to large‐scale high‐throughput technologies. Protein interactions between host and viral proteins are greatly influenced by host signal transduction pathways...
Source: FEMS Immunology and Medical Microbiology - March 13, 2014 Category: Microbiology Authors: Moushimi Amaya, Alan Baer, Kelsey Voss, Catherine Campbell, Claudius Mueller, Charles Bailey, Kylene Kehn‐Hall, Emanuel Petricoin III, Aarthi Narayanan Tags: MiniReview Source Type: research

Multiple effects of Escherichia coli Nissle 1917 on growth, biofilm formation, and inflammation cytokines profile of Clostridium perfringens type A strain CP4
In this study, we evaluated the effects of EcN on growth, toxin production, biofilm formation, and inflammatory cytokine responses of C. perfringens. In vitro co‐culture experiments demonstrated that EcN inhibited growth, gas production, and toxin production (α‐toxin and NetB) of C. perfringens in a dose‐dependent manner. The growth inhibition effect was not observed when C. perfringens was incubated with EcN cell‐free supernatants (CFSE), suggesting that growth inhibition was caused by nutrition competition during co‐incubation. In vitro studies demonstrated that pre‐incubation with EcN did not inhibit C.�...
Source: FEMS Immunology and Medical Microbiology - March 11, 2014 Category: Microbiology Authors: Yanlong Jiang, Qingke Kong, Kenneth L. Roland, Amanda Wolf, Roy Curtiss Tags: Research Article Source Type: research

Temporal expression of agrB, cidA, and alsS in the early development of Staphylococcus aureus UAMS‐1 biofilm formation and the structural role of extracellular DNA and carbohydrates
Abstract Extracellular DNA (eDNA) is an important component of the extracellular polymeric substance matrix and is important in the establishment and persistence of Staphylococcus aureus UAMS‐1 biofilms. The aim of the study was to determine the temporal expression of genes involved in early biofilm formation and eDNA production. We used qPCR to investigate expression of agrB, which is associated with secreted virulence factors and biofilm dispersal, cidA, which is associated with biofilm adherence and genomic DNA release, and alsS, which is associated with cell lysis, eDNA release and acid tolerance. The contribution of...
Source: FEMS Immunology and Medical Microbiology - March 11, 2014 Category: Microbiology Authors: Rossella Grande, Laura Nistico, Karthik Sambanthamoorthy, Mark Longwell, Antonio Iannitelli, Luigina Cellini, Antonio Stefano, Luanne Hall Stoodley, Paul Stoodley Tags: Research Article Source Type: research

High‐content phenotypic screenings to identify inhibitors of Candida albicans biofilm formation and filamentation
Abstract Candida species represent the main cause of opportunistic fungal infections worldwide, and Candida albicans remains the most common etiological agent of candidiasis, now the third to fourth most common nosocomial infection. These infections are typically associated with high morbidity and mortality, mainly due to the limited efficacy of current antifungal drugs. In C. albicans, morphogenetic conversions between yeast and filamentous forms and biofilm formation represent two important biological processes that are intimately associated with the biology of this fungus and also play important roles during the pathog...
Source: FEMS Immunology and Medical Microbiology - March 11, 2014 Category: Microbiology Authors: Christopher G. Pierce, Stephen. P. Saville, Jose L. Lopez‐Ribot Tags: Research Article Source Type: research

Treatment of microbial biofilms in the post‐antibiotic era: prophylactic and therapeutic use of antimicrobial peptides and their design by bioinformatics tools
Abstract The treatment for biofilm infections is particularly challenging because bacteria in these conditions become refractory to antibiotic drugs. The reduced effectiveness of current therapies spurs research for the identification of novel molecules endowed with antimicrobial activities and new mechanisms of antibiofilm action. Antimicrobial peptides (AMPs) have been receiving increasing attention as potential therapeutic agents, because they represent a novel class of antibiotics with a wide spectrum of activity and a low rate in inducing bacterial resistance. Over the past decades, a large number of naturally occurri...
Source: FEMS Immunology and Medical Microbiology - March 7, 2014 Category: Microbiology Authors: Mariagrazia Di Luca, Giuseppe Maccari, Riccardo Nifosì Tags: MiniReview Source Type: research

Biocontainment Laboratory Risk Assessment: Perspectives and Considerations
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Source: FEMS Immunology and Medical Microbiology - March 2, 2014 Category: Microbiology Authors: Amy Patterson, Kelly Fennington, Ryan Bayha, Diane Wax, Rona Hirschberg, Nancy Boyd, Michael Kurilla Tags: MiniReview Source Type: research

Systems Kinomics for Characterizing Host Responses to High Consequence Pathogens at the NIH/NIAID Integrated Research Facility‐ Frederick
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Source: FEMS Immunology and Medical Microbiology - March 2, 2014 Category: Microbiology Authors: Jason Kindrachuk, Shane Falcinelli, Jiro Wada, Jens H. Kuhn, Lisa E. Hensley, Peter B. Jahrling Tags: MiniReview Source Type: research

Innate immunity is sufficient for the clearance of Chlamydia trachomatis from the female mouse genital tract
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Source: FEMS Immunology and Medical Microbiology - March 2, 2014 Category: Microbiology Authors: Gail L. Sturdevant, Harlan D. Caldwell Tags: Short Communication Source Type: research

A Personal History of Research on Microbial Biofilms and Biofilm Infections
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Source: FEMS Immunology and Medical Microbiology - March 2, 2014 Category: Microbiology Authors: Niels Høiby Tags: MiniReview Source Type: research

Involvement of toxin‐antitoxin modules in Burkholderia cenocepacia biofilm persistence
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Source: FEMS Immunology and Medical Microbiology - March 1, 2014 Category: Microbiology Authors: Heleen Acker, Andrea Sass, Inne Dhondt, Hans J. Nelis, Tom Coenye Tags: Research Paper Source Type: research