The role of the chlamydial effector CPAF in the induction of genomic instability
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 31, 2014 Category: Microbiology Authors: Scott S Grieshaber, Nicole A Grieshaber Tags: Short Communication Source Type: research

An H1‐H3 Chimeric Influenza Virosome Confers Complete Protection against Lethal Challenge with PR8 (H1N1) and X47 (H3N2) Viruses in Mice
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 28, 2014 Category: Microbiology Authors: Asghar Abdoli, Hoorieh Soleimanjahi, Masoumeh Tavassoti Kheiri, Abbas Jamali, Vahideh Mazaheri, Meghdad Abdollahpour Alitappeh Tags: Research Paper Source Type: research

Myosin IIA is essential for Shigella flexneri cell ‐to‐cell spread
In this study, the role of myosin II and its specific kinase, myosin light chain kinase (MLCK), during Shigella intercellular spreading was investigated in HeLa cells. Inhibition of MLCK and myosin II, as well as myosin IIA knockdown, significantly reduced Shigella plaque and infectious focus formation. Protrusion formation and intracellular bacterial growth was not affected. Low levels of myosin II were localised to the Shigella F‐actin tail. HeLa cells were also infected with Shigella strains defective in cell‐to‐cell spreading. Unexpectedly loss of myosin IIA labelling was observed in HeLa cells infected with thes...
Source: FEMS Immunology and Medical Microbiology - July 24, 2014 Category: Microbiology Authors: Mabel Lum, Renato Morona Tags: Research Article Source Type: research

Detection and functionality of the CdtB, PltA, and PltB from Salmonella enterica serovar Javiana
In this study we report for the first time the functionality of cdtB, pltA and pltB in non‐typhoidal S. Javiana during infection in HeLa cell lines. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 16, 2014 Category: Microbiology Authors: Ezat H. Mezal, Dongryeoul Bae, Ashraf A. Khan Tags: Research Article Source Type: research

Target cell limitation constrains chlamydial load in persistent infections: results from mathematical modelling applied to mouse genital tract infection data
ConclusionsThe limitation of target cells is a key driver of infection dynamics, affecting both the peak of infection and the equilibrium level of persistent infections. Target cell limitation likely plays an important role in the dynamics of human infections as well.This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 15, 2014 Category: Microbiology Authors: Andrew P. Craig, Roger G. Rank, Anne K. Bowlin, Handan Wand, David P. Wilson Tags: Research Paper Source Type: research

Emerging infections, biosecurity and public health
(Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 14, 2014 Category: Microbiology Authors: Gerald Byrne, Kelly Stefano Cole Tags: Editorial Source Type: research

Issue Information
(Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 14, 2014 Category: Microbiology Tags: Issue Information Source Type: research

Biofilm‐specific extracellular matrix proteins of nontypeable Haemophilus influenzae
Abstract Nontypeable Haemophilus influenzae (NTHi), a human respiratory tract pathogen, can form colony biofilms in vitro. Bacterial cells and the amorphous extracellular matrix (ECM) constituting the biofilm can be separated using sonication. The ECM from 24‐ and 96‐h NTHi biofilms contained polysaccharides and proteinaceous components as detected by nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR) spectroscopy. More conventional chemical assays on the biofilm ECM confirmed the presence of these components and also DNA. Proteomics revealed eighteen proteins present in biofilm ECM tha...
Source: FEMS Immunology and Medical Microbiology - July 10, 2014 Category: Microbiology Authors: Siva Wu, Marc M. Baum, James Kerwin, Debbie Guerrero, Simon Webster, Christoph Schaudinn, David VanderVelde, Paul Webster Tags: Research Article Source Type: research

Biofilm ‐specific extracellular matrix proteins of nontypeable Haemophilus influenzae
Abstract Nontypeable Haemophilus influenzae (NTHi), a human respiratory tract pathogen, can form colony biofilms in vitro. Bacterial cells and the amorphous extracellular matrix (ECM) constituting the biofilm can be separated using sonication. The ECM from 24‐ and 96‐h NTHi biofilms contained polysaccharides and proteinaceous components as detected by nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR) spectroscopy. More conventional chemical assays on the biofilm ECM confirmed the presence of these components and also DNA. Proteomics revealed eighteen proteins present in biofilm ECM tha...
Source: FEMS Immunology and Medical Microbiology - July 9, 2014 Category: Microbiology Authors: Siva Wu, Marc M. Baum, James Kerwin, Debbie Guerrero, Simon Webster, Christoph Schaudinn, David VanderVelde, Paul Webster Tags: Research Article Source Type: research

A comparison of cell mediators and serum cytokines transcript expression between male and female mice infected with Mycobacterium avium subspecies paratuberculosis and/or consuming probiotics
Abstract The gut immune system is complex, and dysregulation leads to a number of disorders including inflammatory bowel syndrome and (in livestock) Johne's disease. Previous work has demonstrated that males and females respond differently to treatment with pathologic and probiotic microorganisms, suggesting that a ‘one‐size‐fits‐all’ approach to treat GIT inflammation may be inadequate. While we had observed significant differences between males and females in terms of cytokine production, it remains unclear how these changes occur. To better understand the mechanisms, transcript expression of genes important to...
Source: FEMS Immunology and Medical Microbiology - July 9, 2014 Category: Microbiology Authors: Enusha Karunasena, Kevin W. McMahon, Paresh C. Kurkure, Mindy M. Brashears Tags: Research Article Source Type: research

Development of imaging techniques to study the pathogenesis of BSL 2/3 infectious agents
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 2, 2014 Category: Microbiology Authors: Courtney E. Rella, Nancy Ruel, Eliseo A. Eugenin Tags: Research Paper Source Type: research

The Mycobacterium tuberculosis Recombinant LprN Protein of mce4 operon Induces Th‐1 Type Response Deleterious to Protection in mice
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 2, 2014 Category: Microbiology Authors: Rashmi Pasricha, Neeraj Kumar Saini, Nisha Rathor, Rakesh Pathak, Rajesh Sinha, Mandira Varma‐Basil, Kiran Mishra, Vani Brahmachari, Mridula Bose Tags: Research Paper Source Type: research

A bovine model of a respiratory Parachlamydia acanthamoebae infection
This study aimed to evaluate the pathogenicity of Parachlamydia (P.) acanthamoebae as a potential agent of lower respiratory tract disease in a bovine model of induced lung infection. Intrabronchial inoculation with P. acanthamoebae was performed in healthy calves aged 2 to 3 months using two challenge doses: 108 and 1010 bacteria per animal. Controls received 108 heat inactivated bacteria. While challenge with 108 viable Parachlamydia resulted in a mild degree of general indisposition, 1010 bacteria induced a more severe respiratory illness becoming apparent 1 – 2 days post inoculation (dpi), affecting 9/9 (100%) anim...
Source: FEMS Immunology and Medical Microbiology - July 2, 2014 Category: Microbiology Authors: Markus Lohr, Annette Prohl, Carola Ostermann, Elisabeth Liebler‐Tenorio, Wieland Schroedl, Sébastien Aeby, Gilbert Greub, Petra Reinhold Tags: Research Paper Source Type: research

The Role of the IKK Complex in Viral Infections
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 1, 2014 Category: Microbiology Authors: Moushimi Amaya, Forrest Keck, Charles Bailey, Aarthi Narayanan Tags: MiniReview Source Type: research

Decreased in vivo virulence and altered gene expression by a Brucella melitensis light‐sensing histidine kinase mutant
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - July 1, 2014 Category: Microbiology Authors: Christopher R. Gourley, Erik Petersen, Jerome Harms, Gary Splitter Tags: Research Paper Source Type: research