FEMS Immunology and Medical Microbiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Issue Information
(Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - May 20, 2013 Category: Microbiology Tags: Issue Information Source Type: research

The responses of γδ T‐cells against acute Pseudomonas aeruginosa pulmonary infection in mice via interleukin‐17
Abstract Interleukin (IL)‐17‐producing T‐lymphocytes play a crucial role in inflammation, yet the potential roles of the cells in acute bacterial pulmonary infection remain unclear. Here, we investigated the role of IL‐17‐producing γδ T‐cells in a mouse model of acute Pseudomonas aeruginosa pulmonary infection. Results showed that augmentation of IL‐17, IL‐22 and IL‐23 was associated with the development of acute bacterial pulmonary infection. However, IL‐17 was markedly reduced following the blockade of γδ T‐cell activity in vivo. The levels of the chemokines, including granulocyte colony‐stimu...
Source: FEMS Immunology and Medical Microbiology - May 15, 2013 Category: Microbiology Authors: Jialin Liu, Hongping Qu, Qingyun Li, Liang Ye, Guanhua Ma, Huanying Wan Tags: Research Article Source Type: research

Genome Sequences of 65 Helicobacter pylori Strains Isolated from Asymptomatic Individuals and Patients with Gastric Cancer, Peptic Ulcer Disease, or Gastritis
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - May 9, 2013 Category: Microbiology Authors: Thomas G. Blanchard, Steven J. Czinn, Pelayo Correa, Teruko Nakazawa, Monika Keelan, Lindsay Morningstar, Ivette Santana‐Cruz, Ankit Maroo, Carri McCracken, Kent Shefchek, Sean Daugherty, Yang Song, Claire M. Fraser, W. Florian Fricke Tags: Short Communication Source Type: research

Codon optimisation to improve expression of a Mycobacterium avium ssp. paratuberculosis‐specific membrane‐associated antigen by Lactobacillus salivarius
Abstract Subunit and DNA‐based vaccines against Mycobacterium avium ssp. paratuberculosis (MAP) attempt to overcome inherent issues associated with whole‐cell formulations. However, these vaccines can be hampered by poor expression of recombinant antigens from a number of disparate hosts. The high G+C content of MAP invariably leads to a codon bias throughout gene expression. To investigate if the codon bias affects recombinant MAP antigen expression, the open reading frame of a MAP‐specific antigen MptD (MAP3733c) was codon optimised for expression against a Lactobacillus salivarius host. Of the total 209 codons whi...
Source: FEMS Immunology and Medical Microbiology - April 18, 2013 Category: Microbiology Authors: Christopher Johnston, Pierre E. Douarre, Tewfik Soulimane, Daniel Pletzer, Helge Weingart, John MacSharry, Aidan Coffey, Roy D. Sleator, Jim O'Mahony Tags: Research Article Source Type: research

Resistance to the quorum‐quenching compounds brominated furanone C‐30 and 5‐fluorouracil in Pseudomonas aeruginosa clinical isolates
Abstract The quorum‐quenching compounds brominated furanone C‐30 and 5‐fluorouracil inhibit the pathogenicity of the Pseudomonas aeruginosa laboratory strains PA01 and PA14; however, there is no report studying the effectiveness of these compounds for clinical isolates. Therefore, the effect of both quorum quenchers on the production of pyocyanin, elastase and alkaline protease of eight clinical strains from children was evaluated. Although both compounds were in general effective for the attenuation of these factors, three strains resistant to C‐30 were found. For 5‐fluorouracil, PA01 and some clinical isolates ...
Source: FEMS Immunology and Medical Microbiology - April 17, 2013 Category: Microbiology Authors: Rodolfo García‐Contreras, Mariano Martínez‐Vázquez, Norma Velázquez Guadarrama, Alejandra Guadalupe Villegas Pañeda, Takahiro Hashimoto, Toshinari Maeda, Héctor Quezada, Thomas K. Wood Tags: Short Communication Source Type: research

Irradiated wild type and Spa mutant Staphylococcus aureus induce anti‐S. aureus immune responses in mice which do not protect against subsequent intravenous challenge
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - April 15, 2013 Category: Microbiology Authors: Pauline M. Diemen, Yuko Yamaguchi, Gavin K. Paterson, Christine S. Rollier, Adrian V.S. Hill, David H. Wyllie Tags: Research Paper Source Type: research

The long polar fimbriae operon and its flanking regions in bovine Escherichia coli O157:H43 and STEC O136:H12 strains
Abstract Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2‐1 operon (lpf2ABCD) and its flanking regions of an intimin‐ and Shiga toxin‐negative atypical E. coli O157:H43 strain of bovine origin and also sequenced the lpf2‐1 operon of six additional atypical O157 bovine E. coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 E. coli Refer...
Source: FEMS Immunology and Medical Microbiology - April 11, 2013 Category: Microbiology Authors: Domonkos Sváb, Lucia Galli, Balázs Horváth, Gergely Maróti, Ulrich Dobrindt, Alfredo G. Torres, Marta Rivas, István Tóth Tags: Research Article Source Type: research

Issue Information
(Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - April 8, 2013 Category: Microbiology Tags: Issue Information Source Type: research

Retnla down‐regulation and IL‐13‐rich environment correlate with inflammation severity in experimental actinomycetoma by Nocardia brasiliensis
Abstract Nocardia brasiliensis (Nb) is a facultative intracellular pathogen that may cause actinomycetoma when immune response is unable to control the pathogenic invasion. We used comparative real‐time PCR to evaluate the expression level of molecules indicative of either classical or alternative activation of macrophages, as well as of cytokines involved in macrophage polarization, during the experimental infection in BALB/c mice. We found induction or increased expression of the pro‐inflammatory markers csf2/GM‐CSF, interferon‐gamma, and nos2/iNOS. The expression of Ym1 and IL‐13, which are usually related wit...
Source: FEMS Immunology and Medical Microbiology - April 3, 2013 Category: Microbiology Authors: Irene Meester, Adrián G. Rosas‐Taraco, Mario C. Salinas‐Carmona Tags: Research Article Source Type: research

Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa
Abstract Biofilm formation by Staphylococcus epidermidis is a cause of infections related to peritoneal dialysis (PD). We have used a PD catheter flow‐cell model in combination with confocal scanning laser microscopy and atomic force microscopy to study biofilm formation by S. epidermidis. Adherence to serum‐coated catheters was four times greater than to uncoated ones, suggesting that S. epidermidis binds to serum proteins on the catheter surface. Pseudomonas aeruginosa biofilm supernatant interfered with the formation of a serum protein coat thereby reducing the capacity for biofilm formation in S. epidermidis. Su...
Source: FEMS Immunology and Medical Microbiology - April 3, 2013 Category: Microbiology Authors: Maria Pihl, Anna Arvidsson, Marie Skepö, Martin Nilsson, Michael Givskov, Tim Tolker‐Nielsen, Gunnel Svensäter, Julia R. Davies Tags: Research Article Source Type: research

Codon Optimisation to Improve Expression of a Mycobacterium avium subsp. paratuberculosis Specific Membrane Associated Antigen by Lactobacillus salivarius
Abstract Subunit and DNA based vaccines against Mycobacterium avium subspecies paratuberculosis (MAP) attempt to overcome inherent issues associated with whole‐cell formulations. However, these vaccines can be hampered by poor expression of recombinant antigens from a number of disparate hosts. The high G+C content of MAP invariably leads to a codon bias throughout gene expression. To investigate if the codon bias affects recombinant MAP antigen expression, the open reading frame of a MAP specific antigen MptD (MAP3733c) was codon optimised for expression against a Lactobacillus salivarius host. Of the total 209 codons w...
Source: FEMS Immunology and Medical Microbiology - April 2, 2013 Category: Microbiology Authors: C. Johnston, P.E. Douarre, T. Soulimane, D. Pletzer, H. Weingart, J. MacSharry, A. Coffey, R.D. Sleator, J. O'Mahony Tags: Research Paper Source Type: research

Hepatic damage caused by Coxsackievirus B3 is dependent on age‐related tissue tropisms associated with the Coxsackievirus‐adenovirus receptor
This study was designed to explore the role of coxsackievirus‐adenovirus receptor (CAR) in pathogenesis of CVB3 infected hepatocyte via in vitro and mice study. CVB3 (CVB3/2630) was isolated from liver tissue of a neonate with fulminant hepatitis. Cell lines A549, HeLa, HEp2 and Huh‐7 were maintained in Dulbecco's modified Eagle's medium. One‐day‐old or seven‐day‐old mice progeny were used in the experiments. Viraemia was noted in seven‐day‐old ICR mice 2 hours of an intraperitoneal injection. Thereafter the highest viral titers were detected in blood, liver and spleen. Histopathological studies of the live...
Source: FEMS Immunology and Medical Microbiology - April 1, 2013 Category: Microbiology Authors: Jung‐Yen Liu, Shih‐Min Wang, I‐Chun Chen, Chun‐Keung Yu, Ching‐Chuan Liu Tags: Research paper Source Type: research

The Responses of γδ T‐Cell Against Acute Pseudomonas aeruginosa Pulmonary Infection In Mice Via IL‐17
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - April 1, 2013 Category: Microbiology Authors: Jialin Liu, Hongping Qu, Qingyun Li, Liang Ye, Guanhua Ma, Huanying Wan Tags: Research Paper Source Type: research

Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT
This article is protected by copyright. All rights reserved. (Source: FEMS Immunology and Medical Microbiology)
Source: FEMS Immunology and Medical Microbiology - April 1, 2013 Category: Microbiology Authors: Kimito Hirai, Hiroshi Maeda, Kazuhiro Omori, Tadashi Yamamoto, Susumu Kokeguchi, Shogo Takashiba Tags: Research Paper Source Type: research

IgG subclass profiles in normal human sera of antibodies specific to five kinds of microbial antigens
Abstract The levels of subclass‐specific immunoglobulin G (IgG) antibodies to five microbial antigens in normal human sera were measured by enzyme‐linked immunosorbent assays (ELISA). The aim was to compare the IgG subclass profiles of specific antibodies to diverse antigens originating from virus, intra‐ and extracellular bacteria. Serum samples from 162 Danish women were analyzed for IgG1, IgG2, IgG3, and IgG4 antibodies against a peptide of the major outer membrane protein (MOMP) of Chlamydia trachomatis, the native outer membrane proteins of Chlamydia pneumoniae, recombinant membrane proteins of Mycoplasma pneumo...
Source: FEMS Immunology and Medical Microbiology - March 25, 2013 Category: Microbiology Authors: Astrid Hjelholt, Gunna Christiansen, Uffe S. Sørensen, Svend Birkelund Tags: Research Article Source Type: research