Neuropilin-1 (NRP-1)/GIPC1 pathway mediates glioma progression
This study demonstrated that NRP-1/GIPC1 pathway plays a vital role in glioma progression, and it is a potential important target for multi-gene combined therapeutics. (Source: Tumor Biology)
Source: Tumor Biology - July 31, 2016 Category: Cancer & Oncology Source Type: research
Oral pathogens change proliferation properties of oral tumor cells by affecting gene expression of human defensins
AbstractThe impact of oral pathogens onto the generation and variability of oral tumors has only recently been investigated. To get further insights, oral cancer cells were treated with pathogens and additionally, as a result of this bacterial cellular infection, with human defensins, which are as anti-microbial peptide members of the innate immune system. After cell stimulation, proliferation behavior, expression analysis of oncogenic relevant defensin genes, and effects on EGFR signaling were investigated. The expression of oncogenic relevant anti-microbial peptides was analyzed with real-time PCR and immunohistochemistr...
Source: Tumor Biology - July 31, 2016 Category: Cancer & Oncology Source Type: research
CA27.29 as a tumour marker for risk evaluation and therapy monitoring in primary breast cancer patients
AbstractSeveral trials showed that tumour markers are associated with an impaired prognosis for breast cancer. Whether earlier treatment can improve the course of the disease remains controversial. The SUCCESS Trial compares FEC (500/100/500)-docetaxel (100) vs. FEC (500/100/500)-docetaxel/gemcitabine (75/2000) as well as 2 vs. 5 years of zoledronate in high-risk primary breast cancer patients. In 2669 patients, CA27.29 was measured before and after chemotherapy with the ST AIA-PACK CA27.29 reagent for the AIA-600II automated enzyme immunoassay (Tosoh Bioscience, Belgium). Values above 31 U/ml were considered positive. ...
Source: Tumor Biology - July 31, 2016 Category: Cancer & Oncology Source Type: research
miRNA-451 inhibits glioma cell proliferation and invasion by downregulating glucose transporter 1
< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > MicroRNAs play an important role in tumor development and progression. Tumor growth is closely associated with glucose metabolism. Specifically, tumor cells produce energy (ATP) under aerobic and anaerobic conditions through glycolysis and metabolites, such as lactic acid and ATP, as a result of the Warburg effect. However, the transport of glucose into cells depends on protein transporters in the cell membrane. Therefore, this area has recently become a topic of interest for research on targeted cancer therapy. We found that miRNA-451 inhibits the...
Source: Tumor Biology - July 30, 2016 Category: Cancer & Oncology Source Type: research
A guide for the analysis of long-term population growth in cancer
< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > Although cancer is a chronic disease, most of the in vitro experiments to assess the effectiveness of intervention are performed in hours or a few days. Moreover, none of the available methodologies to measure cell proliferation are adapted to provide information about the growth kinetic during and after treatment. Thus, the objective of this work is to provide a guide to assess long-term changes in cell population size to be used mainly in cancer research. Cumulative population doubling (CPD) graphs based on cell counting for in vitro or tumor vol...
Source: Tumor Biology - July 30, 2016 Category: Cancer & Oncology Source Type: research
Different expression levels of glycans on leukemic cells —a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid
< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > Sialic acid (SA) is normally expressed on the cell membranes and is located at the terminal position of the sugar chains. SA plays an important role for regulation of the innate immunity, function as markers of the cells and can be recognized by a variety of receptors. Interestingly, the level of SA expression is increased on metastatic cancer cells. The availability of specific antibodies against SA is limited and, therefore, biomarker tools for detection of SA are lacking. We have recently presented a novel method for specific fluorescence labeli...
Source: Tumor Biology - July 30, 2016 Category: Cancer & Oncology Source Type: research
Knockdown of miR-182 promotes apoptosis via regulating RIP1 deubiquitination in TNF- α-treated triple-negative breast cancer cells
In this study, up-regulation of miR-182 was validated in TNBC patients and cell lines. Knockdown of miR-182 was observed to hinder the proliferation of BT-549 cells. More importantly, knockdown of miR-182 significantly promoted the apoptosis induced by TNF-α treatment in BT-549 . JC-1 staining and western blot assays revealed that the K63-linked ubiquitin chains on receptor-interacting protein 1 (RIP1) were removed and the outer mitochondrial membrane potential (MMP) and permeability was altered upon combination of TNF-α with anti-miR-182. We then demonstrated that knockd own of miR-182 up-regulated the expression of cyl...
Source: Tumor Biology - July 29, 2016 Category: Cancer & Oncology Source Type: research
Interactions between FGFR2 and RSK2 —implications for breast cancer prognosis
< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > We have previously demonstrated that fibroblast growth factor receptor 2 (FGFR2) activates ribosomal s6 kinase 2 (RSK2) in mammary epithelial cells and that this pathway promotes in vitro cell growth and migration. Potential clinical significance of FGFR2 and RSK2 association has never been investigated. Herein, we have undertaken an evaluation of a possible relationship between FGFR2/RSK2 interdependence and disease outcome in breast cancer (BCa) patients. The clinical analysis was complemented by an in vitro investigation of an involvement of RSK...
Source: Tumor Biology - July 29, 2016 Category: Cancer & Oncology Source Type: research
Inhibition on Numb/Notch signal pathway enhances radiosensitivity of lung cancer cell line H358
The objective of the study is to investigate the effects of the Numb/Notch signal pathway on the radiosensitivity of lung cancer cell line H358. MTT assay and colony forming assay were used to detect the effects of different doses of X-rays and MW167 on the in vitro proliferation of the lung cancer cell line H358. Flow cytometry was applied to evaluate the effects of X rays on the apoptosis of H358. Scratch assay and Transwell invasion assay were used to examine the effects of X-rays on the migration and invasion abilities of H358. The mRNA and protein expressions in the signal pathway were detected by real-time PCR and we...
Source: Tumor Biology - July 29, 2016 Category: Cancer & Oncology Source Type: research
Growth inhibitory effect of rapamycin in Hodgkin-lymphoma cell lines characterized by constitutive NOTCH1 activation
< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > Growing evidence suggests that deregulation of signalling elements of Notch and mammalian target of rapamycin (mTOR) pathways contribute to tumorigenesis. These signals play important roles in cellular functions and malignancies. Their tumorigenic role in T-cell acute lymphoblastic leukaemia (T-ALL) is well known; however, their potential interactions and functions are poorly characterized in Hodgkin lymphoma (HL). The aim of our study was to characterize mTOR and Notch signalling elements in HL cell lines (DEV, L1236, KMH2) and human biopsies and ...
Source: Tumor Biology - July 28, 2016 Category: Cancer & Oncology Source Type: research
Total and mutated EGFR quantification in cell-free DNA from non-small cell lung cancer patients detects tumor heterogeneity and presents prognostic value
< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > Mutation analysis of epidermal growth factor receptor (EGFR) gene is essential for treatment selection in non-small cell lung cancer (NSCLC). Analysis is usually performed in tumor samples. We evaluated the clinical utility of < em class= " a-plus-plus " > EGFR < /em > analysis in plasma cell-free DNA (cfDNA) from patients under treatment with EGFR inhibitors. We selected 36 patients with NSCLC and < em class= " a-plus-plus " > EGFR < /em > -activating mutations. Blood samples were collected at baseline and during treatment with EGFR inhibitors. W...
Source: Tumor Biology - July 28, 2016 Category: Cancer & Oncology Source Type: research
Genomic imbalances and microRNA transcriptional profiles in patients with mycosis fungoides
< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > Mycosis fungoides is the most common type of primary cutaneous T cell lymphoma. We have evaluated < em class= " a-plus-plus " > CDKN2A < /em > losses and < em class= " a-plus-plus " > MYC < /em > gains/amplifications by FISH analysis, as well as expression of < em class= " a-plus-plus " > miR-155 < /em > and members of the oncogenic cluster < em class= " a-plus-plus " > miR-17-92 < /em > ( < em class= " a-plus-plus " > miR17 < /em > , < em class= " a-plus-plus " > miR18a < /em > , < em class= " a-plus-plus " > miR19b < /em > , and < em class= "...
Source: Tumor Biology - July 28, 2016 Category: Cancer & Oncology Source Type: research
Dimethylfumarate inhibits melanoma cell proliferation via p21 and p53 induction and bcl-2 and cyclin B1 downregulation
We examined the adhesion of melanoma cells on lymphendothelial cells during DMF treatment and found a significant reduction in interaction. These data provide evidence that DMF inhibits melanoma proliferation by reinduction of important cell cycle inhibitors leading to a concentration-dependent G0/G1 or G2/M cell cycle arrest and induction of apoptosis via downregulation of bcl-2 and induction of p53 and PARP-1 cleavage. Hence, DMF might be an interesting agent in the treatment of melanoma and is worth further investigation in vivo. < /p > (Source: Tumor Biology)
Source: Tumor Biology - July 28, 2016 Category: Cancer & Oncology Source Type: research
ASPP2 suppresses stem cell-like characteristics and chemoresistance by inhibiting the Src/FAK/Snail axis in hepatocellular carcinoma
In this study, we report that downregulation of ASPP2, a member of the ankyrin-repeat-containing, SH3-domain-containing, and proline-rich-region-containing protein (ASPP) family, bestowed HCC cells with stem-like properties and resistance to chemotherapy, including the expansion of si de population fractions, formation of hepatospheroids, expression of stem cell-associated genes, loss of chemosensitivity, and increased tumorigenicity in immunodeficient mice. An expression profiling assay revealed that ASPP2 specifically repressed focal adhesion kinase (FAK)/Src/extracellular sign al regulated kinase (ERK) signaling. ASPP2 ...
Source: Tumor Biology - July 28, 2016 Category: Cancer & Oncology Source Type: research
A novel VHL α isoform inhibits Warburg effect via modulation of PKM splicing
< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > Von Hippel-Lindau ( < em class= " a-plus-plus " > VHL < /em > ) is the most frequently mutated gene in clear cell renal carcinoma. Here, we identified a novel translational variant of VHL, termed VHL α, initiated from an alternative translational start site upstream and in frame with the ATG start codon. We showed that VHLα interacts with and regulates heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1), which consequently modulates pyruvate kinase transcript splicing and reprograms cellu lar glucose metabolism. Our study demonstrated that a...
Source: Tumor Biology - July 28, 2016 Category: Cancer & Oncology Source Type: research
