Extracellular HSP70-peptide complexes promote the proliferation of hepatocellular carcinoma cells via TLR2/4/JNK1/2MAPK pathway
AbstractHeat shock protein 70 (HSP70) and HSP70-peptide complexes (HSP70-PCs) have been implicated in the pathogenesis of multiple tumors in humans and have been experimentally shown to increase the proliferation of cell lines derived from hepatocellular carcinoma. The goal of this study was to elucidate the molecular mechanisms through which extracellular HSP70/HSP70-PCs stimulate the proliferation of hepatocellular carcinoma (HCC). The molecular mechanisms of HSP70/HSP70-PC action were studied in the human hepatocellular carcinoma cell lines HepG2 and Huh-7, as well as tumor tissue collected from patients with HCC (n =Â...
Source: Tumor Biology - August 3, 2016 Category: Cancer & Oncology Source Type: research
Disruption of clock gene expression in human colorectal liver metastases
AbstractThe circadian timing system controls about 40 Â % of the transcriptome and is important in the regulation of a wide variety of biological processes including metabolic and proliferative functions. Disruption of the circadian clock could have significant effect on human health and has an important role in the development of cancer. Here, we comp ared the expression levels of core clock genes in primary colorectal cancer (CRC), colorectal liver metastases (CRLM), and liver tissue within the same patient. Surgical specimens of 15 untreated patients with primary CRC and metachronous CRLM were studied. Quantitative real...
Source: Tumor Biology - August 3, 2016 Category: Cancer & Oncology Source Type: research
MicroRNA-15a inhibits the growth and invasiveness of malignant melanoma and directly targets on CDCA4 gene
In conclusion, miRNA 15a suppressed the growth and invasiveness of melanoma cells, suggesting that miRNA 15a may represent a viable microRNA-based therapy against melanoma. (Source: Tumor Biology)
Source: Tumor Biology - August 3, 2016 Category: Cancer & Oncology Source Type: research
IQGAP1 gene silencing induces apoptosis and decreases the invasive capacity of human hepatocellular carcinoma cells
In this study,IQGAP1 expression was partially silenced in human hepatocellular carcinoma (HepG2) cells. We investigated the impact ofIQGAP1 silencing on the interactions of IQGAP and RAS with several apoptotic proteins, including caspase-3 (CASP3), BCL2-associated X protein (BAX), and B-cell leukemia/lymphoma 2 (BCL2). Additionally, we investigated the effects of the interactions of these genes on cell viability, proliferation, apoptosis, and invasive capacity. IQGAP1 siRNA-treated HepG2 cells showed lower invasive capacity than the control cells, and this reduction was time- and vector concentration-dependent. In addition...
Source: Tumor Biology - August 2, 2016 Category: Cancer & Oncology Source Type: research
Cytosolic Ku70 regulates Bax-mediated cell death
AbstractThe first known function of Ku70 is as a DNA repair factor in the nucleus. Using neuronal neuroblastoma cells as a model, we have established that cytosolic Ku70 binds to the pro-apoptotic protein Bax in the cytosol and blocks Bax ’s cell death activity. Ku70-Bax binding is regulated by Ku70 acetylation in that when Ku70 is acetylated Bax dissociates from Ku70, triggering cell death. We propose that Ku70 may act as a survival factor in these cells such that Ku70 depletion triggers Bax-dependent cell death. Here, we addresse d two fundamental questions about this model: (1) Does all Bax, which is a cytosolic prote...
Source: Tumor Biology - August 2, 2016 Category: Cancer & Oncology Source Type: research
Proteomic analysis of differentially expressed proteins in vitreous humor of patients with retinoblastoma using iTRAQ-coupled ESI-MS/MS approach
AbstractThere is close proximity of vitreous humor with the tumor bulk in eyes with retinoblastoma. This renders vitreous humor a promising source to evaluate disease-specific protein targets in retinoblastoma. We studied the differential proteome of vitreous fluid in retinoblastoma tumors (n = 4) as compared to controls (n = 4). The vitreous humor was depleted off the high abundant fraction using MARS-6 affinity column. Subsequently, the tryptic peptides were derivatised with iTRAQ labels. The labelled peptides were pooled and subjected to fractionation using bRPLC. This was followed by protein identification and q ua...
Source: Tumor Biology - August 2, 2016 Category: Cancer & Oncology Source Type: research
Tumor suppressor Fbxw7 antagonizes WNT signaling by targeting β-catenin for degradation in pancreatic cancer
AbstractPancreatic cancer is one of the deadliest solid malignancies associated with aberrant Wnt signaling activation.Fbxw7 mutations have been implicated in the development of pancreatic cancer, whereas the exact mechanism of this ubiquitin ligase as a tumor suppressor remains unclear in pancreatic carcinogenesis. Here, we describe that Fbxw7 is downregulated upon pancreatic cancer development. Depletion of Fbxw7 results in tumor suppression in pancreatic cancer cells, while Fbxw7 overexpression inhibits pancreatic cancer cell proliferation and invasion. Considering the negative correlation between Fbxw7 and β-catenin, ...
Source: Tumor Biology - August 2, 2016 Category: Cancer & Oncology Source Type: research
Clinical significance of β2-adrenergic receptor expression in patients with surgically resected gastric adenocarcinoma
AbstractThe β2-adrenergic receptor (β2-AR) is highly expressed in various human neoplasms and has been considered a novel therapeutic target of cancer treatment. However, the clinicopathological significance of β2-AR expression in patients with gastric cancer (GC) remains unclear. The aim of this study was t o explore β2-AR expression and its prognostic significance. A total of 331 patients with surgically resected GC were evaluated. Tumor sections were stained immunohistochemically for β2-AR. And, we confirmed β2-AR expression in the GC cell lines by Western blot. β2-AR was highly expressed in 30.5  % of GC patien...
Source: Tumor Biology - August 2, 2016 Category: Cancer & Oncology Source Type: research
Construction and application of a lung cancer stem cell model: antitumor drug screening and molecular mechanism of the inhibitory effects of sanguinarine
This study aimed to develop lung CSCs that stably express stem cell properties through transfection t o further screen traditional Chinese herbal compounds. Lung adenocarcinoma stem cells, which include various phenotypic subgroups, are normally characterized by high expression levels of pluripotent stem cell genes, particularly Nanog and OCT4. Plasmids containing Nanog and OCT4 were constructed and transfected into cells, and lung CSCs were identified not only in vitro using RT-PCR, Western blotting, plate cloning, sphere formation, drug resistance, and transwell migration but also in vivo using a nude mouse tumorigenicit...
Source: Tumor Biology - August 1, 2016 Category: Cancer & Oncology Source Type: research
Identification of novel biomarkers associated with poor patient outcomes in invasive breast carcinoma
AbstractBreast carcinoma (BC) corresponds to 23 Â % of all cancers in women, with 1.38 million new cases and 460,000 deaths worldwide annually. Despite the significant advances in the identification of molecular markers and different modalities of treatment for primary BC, the ability to predict its metastatic behavior is still limited. The purpo se of this study was to identify novel molecular markers associated with distinct clinical outcomes in a Brazilian cohort of BC patients. We generated global gene expression profiles using tumor samples from 24 patients with invasive ductal BC who were followed for at least 5Â yea...
Source: Tumor Biology - August 1, 2016 Category: Cancer & Oncology Source Type: research
Tescalcin expression contributes to invasive and metastatic activity in colorectal cancer
This study demonstrates that TESC is involved in cell migration, invasion, and EMT during CRC tumor invasion. These results implicate TESC as a metastatic mediator and provide a biological rationale for the adverse prognosis associated with elevated TESC expression in human CRC. (Source: Tumor Biology)
Source: Tumor Biology - August 1, 2016 Category: Cancer & Oncology Source Type: research
Unexpected frequency of genomic alterations in histologically normal colonic tissue from colon cancer patients
In conclusion, our approach allowed us to uncover for the first time an unexpected frequency of genetic alteration in normal tissue, suggesting that tumorigenic genetic lesions are already present in histologically normal colonic tissue and that the use in array comparative genomic hybridization (CGH) studies of normal samples as reference for the paired tumors can lead to misrepresented genomic data, which may be incomplete or limited, especially if used for the research of target molecules for personalized therapy and for the possible correlation with clinical outcome. (Source: Tumor Biology)
Source: Tumor Biology - August 1, 2016 Category: Cancer & Oncology Source Type: research
Mucin-1 correlates with survival, smoking status, and growth patterns in lung adenocarcinoma
In conclusion, MUC1 participates in the pathogenesis of lung adenocarcinoma and associates with smoking both in vitro and in vivo. In lung adenocarcinoma, depolarized MUC1 protein expression correlated with histological growth patterns, stage, and patient outcome. (Source: Tumor Biology)
Source: Tumor Biology - July 31, 2016 Category: Cancer & Oncology Source Type: research
Rab11a sustains GSK3 β/Wnt/β-catenin signaling to enhance cancer progression in pancreatic cancer
In conclusion, the present study demonstrated that Rab11a promotes aggressiveness of pancreatic cancer through GSK3β/Wnt/β-catenin signaling pathway. (Source: Tumor Biology)
Source: Tumor Biology - July 31, 2016 Category: Cancer & Oncology Source Type: research
PKC iota promotes cellular proliferation by accelerated G1/S transition via interaction with CDK7 in esophageal squamous cell carcinoma
In this study, we clarified a novel role of PKCι on the cell cycle progression and proliferation in ESCC. Western blot and immunohistochemistry (IHC) analysis showed that the expression of PKCι was higher in ESCC tumor tissues and cell lines. Meanwhile, IHC stain revealed that PKCι was positively correlated with clinical pathologic variables such as tumor size, tumor grade, and tumor invasion, as well as ki67. Immunoprecipitation and immunofluorescence assay revealed that PKCι/CDK7 has the physical interaction and were co-located in the cell nucleus. And this direct interaction could increase the phosphorylation level ...
Source: Tumor Biology - July 31, 2016 Category: Cancer & Oncology Source Type: research
