Methylation profiling of ductal carcinoma in situ and its relationship to histopathological features
IntroductionDNA methylation is a well-studied biomarker in invasive breast cancer, but its role in ductal carcinoma in situ (DCIS) is less well characterized. The aims of this study are to assess the methylation profile in DCIS for a panel of well-characterized genes that are frequently methylated in breast cancer, to investigate the relationship of methylation with pathological features, and to perform a proof-of-principle study to evaluate the practicality of methylation as a biomarker in diagnostic DCIS material.
Methods:
Promoter CpG island methylation for a panel of 11 breast cancer-related genes was performed by meth...
Source: Breast Cancer Research - October 21, 2014 Category: Cancer & Oncology Authors: Jia-Min PangSiddhartha DebElena TakanoDavid ByrneNicholas JeneAlice BoulghourjianAnne HollidayEwan MillarC LeeSandra O¿TooleAlexander DobrovicStephen Fox Source Type: research
Anatomical localization of progenitor cells in human breast tissue reveals enrichment of uncommitted cells within immature lobules
Conclusions:
These results reveal underlying differences in the human breast epithelial hierarchy and suggest that with increasing glandular maturity, the epithelial hierarchy also becomes more complex. (Source: Breast Cancer Research)
Source: Breast Cancer Research - October 15, 2014 Category: Cancer & Oncology Authors: Lisa ArendtPatricia KellerAdam SkibinskiKevin GoncalvesStephen NaberRachel BuchsbaumHannah GilmoreSteven ComeCharlotte Kuperwasser Source Type: research
Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis
Conclusion:
This study unravels a detailed role of NFAT5 in mediating metastatic signaling, and provides broad insights into the involvement of Hotair, in particular, by transcriptionally regulating the expression of microRNA(s), in the metastasis of breast cancers. (Source: Breast Cancer Research)
Source: Breast Cancer Research - October 14, 2014 Category: Cancer & Oncology Authors: Jun-Tang LiLi-Feng WangYa-Li ZhaoTao YangWei LiJing ZhaoFeng YuLei WangYan-Ling MengNing-Ning LiuXiao-Shan ZhuChun-Fang GaoLin-Tao JiaAn-Gang Yang Source Type: research
Functional variants at the 21q22.3 locus involved in breast cancer progression identified by screening of genome-wide estrogen response elements
Conclusion:
These findings support the idea that functional variants in the ER?-regulating sequence at 21q22.3 is important in determining breast cancer progression. (Source: Breast Cancer Research)
Source: Breast Cancer Research - October 9, 2014 Category: Cancer & Oncology Authors: Chia-Ni HsiungHou-Wei ChuYuan-Ling HuangWen-Cheng ChouLing-Yueh HuHuan-Ming HsuPei-Ei WuMing-Feng HouJyh-Cherng YuChen-Yang Shen Source Type: research
TP53 mutations detected in circulating tumor cells present in the blood of metastatic triple negative breast cancer patients
Conclusions:
We are able to isolate uncontaminated CTCs and achieve single cell molecular analysis. Our studies showed the presence of different CTC sub-clones in patients with metastatic breast cancer. Some CTCs had the same TP53 mutation as their matching tumor samples although others showed either a different TP53 mutation or the wild type allele. Our results indicate that CTCs could represent a non-invasive source of cancer cells from which to determine genetic markers of the disease progression and potential therapeutic targets. (Source: Breast Cancer Research)
Source: Breast Cancer Research - October 9, 2014 Category: Cancer & Oncology Authors: Sandra FernandezCatherine BinghamPatricia FittipaldiLaura AustinJuan PalazzoGary PalmerKatherine AlpaughMassimo Cristofanilli Source Type: research
Mammographic breast density refines Tyrer-Cuzick estimates of breast cancer risk in high-risk women: findings from the placebo arm of the International Breast Cancer Intervention Study I
Conclusions:
In women at high risk of breast cancer, adjusting percent mammographic density for age and BMI provides additional predictive information to the TC risk score, which already incorporates BMI, age, family history and other classic breast cancer risk factors. Furthermore, simple selection criteria can be developed using mammographic density, age and BMI to identify women at increased risk in a clinical setting.Clinical trial registration numberhttp://www.controlled-trials.com/ISRCTN91879928 (Registered: 1 June 2006). (Source: Breast Cancer Research)
Source: Breast Cancer Research - October 8, 2014 Category: Cancer & Oncology Authors: Jane WarwickHanna BirkeJennifer StoneRuth WarrenElizabeth PinneyAdam BrentnallStephen DuffyAnthony HowellJack Cuzick Source Type: research
Mammographic breast density refines Tyrer-Cuzick estimates of breast cancer risk in high risk women: findings from the placebo arm of the international breast cancer intervention study-I
Conclusion:
In women at high risk of breast cancer, adjusting percent mammographic density for age and BMI provides additional predictive information to the TC risk score, which already incorporates BMI, age, family history and other classic breast cancer risk factors. Furthermore, simple selection criteria can be developed using mammographic density, age and BMI to identify women at increased risk in a clinical setting.Clinical trial registration numberISRCTN91879928 (Registered: 1 June, 2006). (Source: Breast Cancer Research)
Source: Breast Cancer Research - October 8, 2014 Category: Cancer & Oncology Authors: Jane WarwickHanna BirkeJennifer StoneRuth WarrenElizabeth PinneyAdam BrentnallStephen DuffyAnthony HowellJack Cuzick Source Type: research
DNA methylation profiling in the Carolina Breast Cancer Study defines cancer subclasses differing in clinicopathologic characteristics and survival
Conclusions:
This study demonstrates that epigenetic patterns are strongly associated with HR status, subtype, and p53 mutation status, and may show heterogeneity within tumor subclass. Among HR+?breast tumors, a subset exhibiting a gene signature characterized by hypermethylation of developmental genes and poorer clinicopathologic features may have prognostic value, and requires further study. Genes differentially methylated between clinically-important tumor subsets have roles in differentiation, development, and tumor growth and may be critical to establishing and maintaining tumor phenotypes and clinical outcomes. (Sou...
Source: Breast Cancer Research - October 7, 2014 Category: Cancer & Oncology Authors: Kathleen ConwaySharon EdmistonRyan MayPei KuanHaitao ChuChristopher BryantChiu-Kit TseTheresa Swift-ScanlanJoseph GeradtsMelissa TroesterRobert Millikan Source Type: research
Attenuated transforming growth factor beta signaling promotes metastasis in a model of HER2 mammary carcinogenesis
Conclusion:
Our studies provide insights into a novel mechanism by which epithelial TGF? signaling modulates the tumor microenvironment, and by which it is involved in lung metastasis in HER2+ breast cancer patients. The effects of pharmacological targeting of the TGF? pathway in vivo during tumor progression remain controversial. The targeting of TGF? signaling should be a viable option, but because VEGF has a protumorigenic effect on HER2+ tumors, the targeting of this protein could be considered when it is associated with attenuated TGF? signaling. (Source: Breast Cancer Research)
Source: Breast Cancer Research - October 4, 2014 Category: Cancer & Oncology Authors: Sergey NovitskiyElizabeth ForresterMichael PickupAgnieszka GorskaAnna ChytilMary AakreDina PolosukhinaPhilip OwensDilyara YusupovaZhiguo ZhaoFei YeYu ShyrHarold Moses Source Type: research
Risk of contralateral second primary breast cancer according to hormone receptor status in Germany
Conclusions:
HR status of the first primary and age at first diagnosis is relevant for predicting risk of CBC. Particularly, patients with HR-negative FBC had elevated risks. (Source: Breast Cancer Research)
Source: Breast Cancer Research - October 3, 2014 Category: Cancer & Oncology Authors: Carsten RusnerKatharina WolfUlrike Bandemer-GreulichJutta EngelChrista StegmaierBernd HolleczekGabriele Schubert-FritschleAnett TillackAndreas Stang Source Type: research
Trefoil factor 3 promotes metastatic seeding and predicts poor survival outcome of patients with mammary carcinoma AU Query: Please confirm the address additions or amend accordingly.
Conclusions:
TFF3 expression predicts metastasis and poor survival outcome of patients with MC and functionally stimulates cellular invasion and metastasis of ER?+?MC cells. Adjuvant functional inhibition of TFF3 may therefore be considered to ameliorate outcome of ER?+?MC patients. (Source: Breast Cancer Research)
Source: Breast Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Vijay PandeyZheng-Sheng WuMin ZhangRui LiJian ZhangTao ZhuPeter Lobie Source Type: research
Risk determination and prevention of breast cancer
Howell and colleagues from the BCC risk-and-prevention panel report on how adding new, but available, information to current risk models could ultimately better predict a woman’s individual breast cancer risk. (Source: Breast Cancer Research)
Source: Breast Cancer Research - September 28, 2014 Category: Cancer & Oncology Authors: Anthony HowellAnnie AndersonRobert ClarkeStephen DuffyD EvansMontserat Garcia-ClosasAndy GescherTimothy KeyJohn SaxtonMichelle Harvie Source Type: research
Elevated DDX21 regulates c-Jun activity and rRNA processing in human breast cancers
Conclusions:
Our findings indicate that DDX21 expression in breast cancer cells can promote AP-1 activity and rRNA processing, and thus, promote tumorigenesis by two independent mechanisms. DDX21 could serve as a marker for a subset of breast cancer patients with higher proliferation potential and may be used as a therapeutic target for a subset of breast cancer patients. (Source: Breast Cancer Research)
Source: Breast Cancer Research - September 28, 2014 Category: Cancer & Oncology Authors: Yandong ZhangKathleen BaysacLian-Fai YeeAnthony SaporitaJason Weber Source Type: research
Significance of glioma-associated oncogene homolog 1 (GLI1) expression in claudin-low breast cancer and crosstalk with the nuclear factor kappa-light-chain-enhancer of activated B cells (NF¿B) pathway
Conclusions:
This work highlights the importance of GLI1 in the maintenance of characteristics of metastatic breast cancer stem cells. Remarkably, treatment with an inhibitor of the NF?B pathway reproducibly reduces GLI1 expression and protein levels. We further provide direct evidence for the binding of the NF?B subunit p65 to the GLI1 promoter in both EMT and claudin-low cell lines. Our results uncover crosstalk between NF?B and GLI1 signals and suggest that targeting these pathways may be effective against the claudin-low breast cancer subtype. (Source: Breast Cancer Research)
Source: Breast Cancer Research - September 25, 2014 Category: Cancer & Oncology Authors: Sierra ColavitoMike ZouQin YanDon NguyenDavid Stern Source Type: research
Cas proteins: dodgy scaffolding in breast cancer
The members of the Cas protein family (p130Cas/BCAR1, Nedd9/HEF1, EFS and CASS4) are scaffold proteins required for the assembly of signal transduction complexes in response to several stimuli, such as growth factors, hormones and extracellular matrix components. Given their ability to integrate and coordinate multiple signalling events, Cas proteins have emerged as crucial players in the control of mammary cell proliferation, survival and differentiation. More importantly, it has been found that alterations of their expression levels result in aberrant signalling cascades, which promote initiation and progression of breas...
Source: Breast Cancer Research - September 25, 2014 Category: Cancer & Oncology Authors: Giusy TornilloPaola DefilippiSara Cabodi Source Type: research
