Molecular cloning, characterization and expression of immunoglobulin D on pathogen challenge and pathogen associated molecular patterns stimulation in freshwater carp, Catla catla
ABSTRACT
The primordial immunoglobulin class, IgD, was the first non‐IgM isotype discovered in teleosts. The crucial roles of IgM and IgZ in imparting systemic and mucosal immunity, respectively, in various fish species have been widely established. However, the putative function of a unique IgD isotype during pathogenic invasions has not been well explored. The present study reports the existence of an IgD ortholog in freshwater carp, Catla catla, and further evaluates its differential expression profile in response to bacterial, parasitic and viral antigenic exposure and pathogen associated molecular patterns (PAMPs) s...
Source: Microbiology and Immunology - October 12, 2017 Category: Microbiology Authors: Rajanya Banerjee, Bhakti Patel, Madhubanti Basu, Saswati S. Lenka, Mahismita Paicha, Mrinal Samanta, Surajit Das Tags: Original Article Source Type: research
Effects of lactoferrin and lactoperoxidase ‐containing food on the oral microbiota of older individuals
ABSTRACT
The oral microbiota influences health and disease states. Some gram‐negative anaerobic bacteria play important roles in tissue destruction associated with periodontal disease. Lactoferrin (LF) and lactoperoxidase (LPO) are antimicrobial proteins found in saliva; however, their influence on the whole oral microbiota currently remains unknown. In this randomized, double‐blinded, placebo‐controlled study, the effects of long‐term ingestion of LF and LPO‐containing tablets on the microbiota of supragingival plaque and tongue coating were assessed. Forty‐six older individuals ingested placebo or test tablet...
Source: Microbiology and Immunology - October 12, 2017 Category: Microbiology Authors: Manabu Nakano, Hiroyuki Wakabayashi, Hirosuke Sugahara, Toshitaka Odamaki, Koji Yamauchi, Fumiaki Abe, Jin ‐Zhong Xiao, Kohji Murakami, Kentaro Ishikawa, Shouji Hironaka Tags: Original Article Source Type: research
Issue Information – Cover
Cover photograph: Antibodies induced by the recombinant Bordetella pertussis AfuA (rAfuA) promote PMN phagocytosis of Bordetella parapertussis. Bacterial phagocytosis was assessed by confocal laser scan fluorescence microscopy. B. parapertussis was incubated with anti‐rAfuA serum for 30 min at 37°C, washed, and further incubated with PMN. Intracellular bacteria were labeled in green and extracellular bacteria were labeled with both green and red fluorescent dyes. Microbiol Immunol: 61: 407‐415. Article link here. (Source: Microbiology and Immunology)
Source: Microbiology and Immunology - October 12, 2017 Category: Microbiology Tags: Cover Image Source Type: research
MASTDISCS combi Carba plus, a simple discrimination method for carbapenemase ‐producing Enterobacteriaceae including OXA‐48‐type producers
This study aimed to evaluate the performance of the MASTDISCS® combi Carba plus disc system in the identification of different carbapenemase types, including OXA‐48‐type carbapenemase for which no specific enzyme inhibitors have so far been available. The simple disc system exerts its discriminatory power of carbapenemase including OXA‐48‐types exhibiting low carbapenem MICs by targeting Enterobacteriaceae isolates with EUCAST meropenem screening cut‐off of ≥0.25 mg/L. (Source: Microbiology and Immunology)
Source: Microbiology and Immunology - October 1, 2017 Category: Microbiology Authors: Yusuke Ohsaki, Ryoichi Kubo, Jonathan Hobson, Mya Davies, Shunsuke Osaka, Wataru Hayashi, Yui Taniguchi, Shota Koide, Yukiko Nagano, Noriyuki Nagano Tags: Note Source Type: research
Characterization of Leptospira species isolated from soil collected in Japan
ABSTRACT
Leptospira was isolated from soil obtained from Hokkaido, the northernmost island, to Okinawa, the southernmost island, in Japan using sulfamethoxazole, trimethoprim, amphotericin B, fosfomycin, and 5‐ fluorouracil (STAFF). Fifty out of 132 soil samples (37.9%) were culture‐positive. Based on 16S‐rDNA sequences, 12 were classified into a pathogenic species clade and were closely associated with L. alstonii and L. kmetyi. Nine isolates were classified into intermediate species, which were similar to L. licerasiae. Twenty‐seven isolates were classified as non‐pathogenic species. Among them, 23 isolates wer...
Source: Microbiology and Immunology - October 1, 2017 Category: Microbiology Authors: Toshiyuki Masuzawa, Keiko Sakakibara, Mitsumasa Saito, Yusuke Hidaka, Sharon Y. A. M. Villanueva, Yasutake Yanagihara, Shin ‐ichi Yoshida Tags: Note Source Type: research
Molecular typing and occurrence of beta ‐lactam resistance genes of Shigella sonnei strains isolated during 1983‐2014 in the São Paulo State in Brazil
ABSTRACT
Shigella sonnei is generally associated with dysentery in developed countries; but it has recently emerged in developing countries. Specifically, in Brazil there are few published studies that molecularly characterized this species. The aims of this study were to analyze the efficacy of the MLVA typing method, to study the phylogeny by MLST and to assess the presence of some beta‐lactam resistance genes in S. sonnei strains isolated from human diarrhoeic faeces in the São Paulo State in Brazil between 1983‐2014. Seventy‐two S. sonnei strains were typed by MLVA and grouped in two clusters, both presenting is...
Source: Microbiology and Immunology - October 1, 2017 Category: Microbiology Authors: Amanda Ap. Seribelli, Miliane R. Fraz ão, Marta I. Cazentini Medeiros, Eliana G. Stehling, Juliana P. Falcão Tags: Original Article Source Type: research
ANG II ‐CCR2/5 axis dependent monocyte/macrophage recruitment contributed to experimental autoimmune myocarditis progression
ABSTRACT
Angiotensin II (ANG II) plays critical roles in modulation of circulatory homeostasis and activation of innate and adaptive immunity. Furthermore, ANG II has also been implicated in several mouse models of autoimmune disease. However, it remains incompletely clear how ANG II regulates macrophage and involves in experimental autoimmune myocarditis (EAM) development. Therefore, the present work was to address the above question and to explore the possible mechanism. The BALB/c mice were used to induce EAM model. ANG II levels were detected by ELISA; HE staining was employed to analyze the pathological changes and ma...
Source: Microbiology and Immunology - October 1, 2017 Category: Microbiology Authors: Hongxiang Lu, Gangjun Zong, Shanshan Zhou, Yuanyuan Jiang, Rong Chen, Zhaoliang Su, Yan Wu Tags: Original Article Source Type: research
Issue Information – Instruction for Authors
(Source: Microbiology and Immunology)
Source: Microbiology and Immunology - September 20, 2017 Category: Microbiology Tags: Issue Information – Instruction for Authors Source Type: research
Molecular characterization of Streptococcus pneumoniae, particularly serotype19A/ST320, which emerged in Krasnoyarsk, Russia
ABSTRACT
Streptococcus pneumoniae, a common human pathogen, colonizes the nasopharynx and causes diseases including acute otitis media (AOM). Herein, pneumococcal serotype distributions in children before and after PCV7 vaccination and in patients with pneumococcal disease in Siberian Russia (Krasnoyarsk) are reported. Analyses included antimicrobial susceptibility testing, sequence typing (ST), pulsed field gel electrophoresis, virulence‐related surface protein gene (VSG) typing with novel primers and structural analysis by scanning electron microscopy. In healthy children (HC) prior to administration of PCV7, drug‐su...
Source: Microbiology and Immunology - September 20, 2017 Category: Microbiology Authors: Irina N. Protasova, Tsai ‐Wen Wan, Natalya V. Bakhareva, Wei‐Chun Hung, Wataru Higuchi, Yasuhisa Iwao, Tatyana A. Yelistratova, Natalya A. Ilyenkova, Yelena S. Sokolovskaya, Galina P. Martynova, Ivan V. Reva, Galina V. Reva, Sergey V. Sidorenko, Lee Tags: Original Article Source Type: research
Issue Information – Cover
Cover photograph: Immunofluorescence microscopy of Bordetella pertussis 18323 with an antiserum (anti‐SBSPV) against the mixture of five in vivo‐expressed autotransporters (green) and anti‐B. pertussis antibody (magenta). Anti‐SBSPV dominantly recognized the cell surface of B. pertussis. Microbiol Immunol: 61: 371‐379. Article link here. (Source: Microbiology and Immunology)
Source: Microbiology and Immunology - September 20, 2017 Category: Microbiology Tags: Cover Image Source Type: research
Prevalence, comorbidities and mortality of toxic shock syndrome in United States children and adults
In conclusion, significant racial/ethnic, socioeconomic, and comorbid disparities exist in the incidence and mortality due to TSS in US adults and children. (Source: Microbiology and Immunology)
Source: Microbiology and Immunology - September 11, 2017 Category: Microbiology Authors: Mark A. Strom, Derek Y. Hsu, Jonathan I. Silverberg Tags: Original Article Source Type: research
Human papillomavirus prevalence in lung carcinomas in Bulgaria
This study aimed to screen Bulgarian lung cancer patients for the prevalence of HPV16 and 18. We analyzed 209 biopsy specimens from patients with histologically proven lung cancer and non‐cancer cases. Each sample was subjected to three parallel PCRs using broad spectrum GP5+/6+ primers and type‐specific (TS) primers for HPV types 16 and 18. Of the 132 lung carcinoma samples 33(25 %) were positive for HPV16 and/or HPV18 by TS PCR and only 5(3.8%) samples were HPV positive by consensus PCR. All non‐malignant controls were HPV negative. HPV18 was more prevalent, found in 11.4% of samples, followed by HPV16 detected in ...
Source: Microbiology and Immunology - September 7, 2017 Category: Microbiology Authors: Evelina Shikova, Zina Ivanova, Dora Alexandrova, Mihail Shindov, Andrey Lekov Tags: Original Article Source Type: research
Effects of lactoferrin and lactoperoxidase ‐containing food on the oral microbiota in the elderly
Abstract
The oral microbiota influences health and disease states. Some Gram‐negative anaerobic bacteria play an important role in tissue destruction associated with periodontal disease. Lactoferrin (LF) and lactoperoxidase (LPO) are antimicrobial proteins found in saliva; however, their influence on the whole oral microbiota currently remains unknown. We herein assessed the effects of the long‐term ingestion of LF‐ and LPO‐containing tablets on the microbiota of supragingival plaque and tongue coating in a randomized, double‐blinded, placebo‐controlled study. Forty‐six elderly subjects ingested placebo or te...
Source: Microbiology and Immunology - September 7, 2017 Category: Microbiology Authors: Manabu Nakano, Hiroyuki Wakabayashi, Hirosuke Sugahara, Toshitaka Odamaki, Koji Yamauchi, Fumiaki Abe, Jin ‐Zhong Xiao, Kohji Murakami, Kentaro Ishikawa, Shouji Hironaka Tags: Original Article Source Type: research
Genetic characterization of an avian H4N6 influenza virus isolated from the Izumi plain, Japan
ABSTRACT
An influenza A virus of H4N6 subtype was isolated from the Izumi plain, Japan, in 2013. Genetic analyses revealed that two viral genes (M and NS gene segments) of this isolate were genetically distinct from those of the H4N6 virus isolated from the same place in 2012. Furthermore, three viral genes (PB2, PB1, and M gene segments) of our isolate share high similarity with those of the North American isolates of 2014. These results suggest a high frequency of the genetic reassortment of avian influenza viruses in Asian waterfowl and intercontinental movements of avian influenza viruses via migratory waterfowl. (Sour...
Source: Microbiology and Immunology - September 1, 2017 Category: Microbiology Authors: Kosuke Okuya, Norihiro Kanazawa, Takehiro Kanda, Masakazu Kuwahara, Aya Matsuu, Masayuki Horie, Tatsunori Masatani, Shigehisa Toda, Makoto Ozawa Tags: Note Source Type: research
A novel recombinant anti ‐EGFR peptide vaccine capable of active immunization and reduction of tumor volume in mice model
ABSTRACT
Over‐expression of the Epidermal Growth Factor Receptor (EGFR) has been reported in a number of human malignancies. High levels of expression of this receptor have been associated with poor survival in many such patients. Active immunizations that elicit antibodies of the desired type could be an appealing alternative for conventional passive immunization. In this regard, we decide to construct a novel recombinant peptide vaccine capable of prophylactic and therapeutic effects. A novel fusion recombinant peptide base vaccine consisting of L2 domain of murine ECD‐EGFR and EGFR mimotope (EM‐L2) was constructed...
Source: Microbiology and Immunology - September 1, 2017 Category: Microbiology Authors: Majid Asadi ‐Ghalehni, Mohamad Javad Rasaee, Masoumeh RajabiBazl, Masood Khosravani, Majid Motaghinejad, Masoud Javanmardi, Saeed Khalili, Helmout Modjtahedi, Esmaeil Sadroddiny Tags: Original Article Source Type: research
