From enzymology to systems biology and back – Prolog
Publication date: December 2016 Source:Perspectives in Science, Volume 9 Author(s): Carsten Kettner, Martin G. Hicks (Source: Perspectives in Science)
Source: Perspectives in Science - January 7, 2017 Category: Science Source Type: research
Glycan arrays and other tools produced by automated glycan assembly
Publication date: Available online 12 December 2016 Source:Perspectives in Science Author(s): Peter H. Seeberger Carbohydrates are the dominant biopolymer on earth and play important roles ranging from building material for plants to function in many biological systems. Glycans remain poorly studied due to a lack of synthetic tools. The goal of my laboratory has been to develop a general method for the automated assembly of glycans. The general protocols we developed resulted in the commercialisation of the Glyconeer 2.1™ synthesizer as well as the building blocks and all reagents. Oligosaccharides as long as 50-mers ...
Source: Perspectives in Science - December 12, 2016 Category: Science Source Type: research
Impact of defining glycan structures
Publication date: Available online 24 November 2016 Source:Perspectives in Science Author(s): Johannes F.G. Vliegenthart For gaining insight in the mode of action at the molecular level of glycans in biological systems precise knowledge of the structure of the glycans is indispensable. To obtain this fundamental information well-defined starting material, optimal fractionation methods and adequate identification techniques are essential. In this review, the emphasis is on the application of high resolution 1H NMR spectroscopy to the structure determination of glycans of different origin. The power of 1H NMR spectroscopy...
Source: Perspectives in Science - November 24, 2016 Category: Science Source Type: research
Bioinformatic applications to aid high-throughput glycan profiling
This article briefly describes our robotic platform and concentrates on a set of software tools for the interpretation of quantitative glycoprofiling data. (Source: Perspectives in Science)
Source: Perspectives in Science - November 24, 2016 Category: Science Source Type: research
Epilogue —Unravelling Glycan Complexity
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Weston B. Struwe (Source: Perspectives in Science)
Source: Perspectives in Science - November 20, 2016 Category: Science Source Type: research
Unravelling glycan complexity —Prologue
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Carsten Kettner, Martin G. Hicks (Source: Perspectives in Science)
Source: Perspectives in Science - November 15, 2016 Category: Science Source Type: research
2-C-Branched mannosides as a novel family of FimH antagonists —Synthesis and biological evaluation
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Wojciech Schönemann, Marcel Lindegger, Said Rabbani, Pascal Zihlmann, Oliver Schwardt, Beat Ernst Urinary tract infections (UTIs), which are among the most prevalent bacterial infections worldwide, are mainly attributed to uropathogenic Escherichia coli (UPEC). Because of frequent antibiotic treatment, antimicrobial resistance constitutes an increasing therapeutic problem. Antagonists of the mannose-specific bacterial lectin FimH, a key protein mediating the adhesion of UPEC to human bladder cells, would offer an alternati...
Source: Perspectives in Science - November 11, 2016 Category: Science Source Type: research
Complex carbohydrate recognition by proteins: Fundamental insights from bacteriophage cell adhesion systems
Publication date: Available online 10 November 2016 Source:Perspectives in Science Author(s): Nina K. Broeker, Dorothee Andres, Yu Kang, Ulrich Gohlke, Andreas Schmidt, Sonja Kunstmann, Mark Santer, Stefanie Barbirz Protein–glycan interactions are ubiquitous in nature. Molecular description of complex formation and the underlying thermodynamics, however, are not well understood due to the lack of model systems. Bacteriophage tailspike proteins (TSP) possess binding sites for bacterial cell surfaces oligosaccharides. In this article we describe the analysis of TSP-oligosaccharide complexes. TSP provide large gly...
Source: Perspectives in Science - November 10, 2016 Category: Science Source Type: research
GLYDE-II: The GLYcan data exchange format
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Rene Ranzinger, Krys J. Kochut, John A. Miller, Matthew Eavenson, Thomas Lütteke, William S. York The GLYcan Data Exchange (GLYDE) standard has been developed for the representation of the chemical structures of monosaccharides, glycans and glycoconjugates using a connection table formalism formatted in XML. This format allows structures, including those that do not exist in any database, to be unambiguously represented and shared by diverse computational tools. GLYDE implements a partonomy model based on human language al...
Source: Perspectives in Science - October 28, 2016 Category: Science Source Type: research
Epilogue − Unravelling Glycan Complexity
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Weston B. Struwe (Source: Perspectives in Science)
Source: Perspectives in Science - October 28, 2016 Category: Science Source Type: research
Protein –glycosaminoglycan interaction networks: focus on heparan sulfate
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Sylvie Ricard-Blum Sulfated glycosaminoglycans (GAGs) are complex polysaccharides, which are covalently bound to protein cores to form proteoglycans. They are mostly located at the cell surface and in the extracellular matrix (ECM) where they regulate numerous biological processes. The aim of our work is i) to identify and characterize protein–GAG interactions occurring at the cell surface and in the ECM, ii) to study the assembly of multimolecular complexes formed at the cell surface via protein–heparan sulfate interactions...
Source: Perspectives in Science - October 28, 2016 Category: Science Source Type: research
2-C-Branched mannosides as a novel family of FimH antagonists − Synthesis and biological evaluation
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Wojciech Schönemann, Marcel Lindegger, Said Rabbani, Pascal Zihlmann, Oliver Schwardt, Beat Ernst Urinary tract infections (UTIs), which are among the most prevalent bacterial infections worldwide, are mainly attributed to uropathogenic Escherichia coli (UPEC). Because of frequent antibiotic treatment, antimicrobial resistance constitutes an increasing therapeutic problem. Antagonists of the mannose-specific bacterial lectin FimH, a key protein mediating the adhesion of UPEC to human bladder cells, would offer an alternati...
Source: Perspectives in Science - October 28, 2016 Category: Science Source Type: research
Unravelling glycan complexity − prologue
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Carsten Kettner, Martin G. Hicks (Source: Perspectives in Science)
Source: Perspectives in Science - October 28, 2016 Category: Science Source Type: research
Latest developments in Semantic Web technologies applied to the Glycosciences
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Kiyoko F. Aoki-Kinoshita, Nobuyuki P. Aoki, Akihiro Fujita, Noriaki Fujita, Toshisuke Kawasaki, Masaaki Matsubara, Shujiro Okuda, Toshihide Shikanai, Daisuke Shinmachi, Elena Solovieva, Yoshinori Suzuki, Shinichiro Tsuchiya, Issaku Yamada, Hisashi Narimatsu The Integrated Life Science Database Project of Japan funded a group of glycoscientists to carry out a project to integrate glycoscience databases using Semantic Web technologies. As a continuation of the previous project period, the Japan Consortium for Glycobio...
Source: Perspectives in Science - October 28, 2016 Category: Science Source Type: research
Bioinformatics of glycosaminoglycans
Publication date: Available online 20 October 2016 Source:Perspectives in Science Author(s): Han Hu, Yang Mao, Yu Huang, Cheng Lin, Joseph Zaia Cell surface heparan sulfates modulate many signaling pathways by binding growth factors and growth factor receptors. Expressed in a spatially and temporally regulated manner, these highly sulfated polysaccharides play important roles in all aspects of animal physiology. To understand heparan sulfate-protein binding, it is necessary to develop instrumental sequencing methods. Towards this end, we and others have demonstrated the effectiveness of activated electron dissociati...
Source: Perspectives in Science - October 28, 2016 Category: Science Source Type: research
