Issue Information
(Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - November 17, 2017 Category: Laboratory Medicine Tags: ISSUE INFORMATION Source Type: research

Cover Image
On the cover: The cover image, by Hacer Aksel et al., is based on the Research Article Efficacy of different irrigant protocols and application systems on sealer penetration using a stepwise CLSM analysis, DOI: 10.1002/jemt.22944. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - November 17, 2017 Category: Laboratory Medicine Authors: Hacer Aksel, Selen K üçükkaya Eren, Nuhan Puralı, Ahmet Serper, Adham A. Azim Tags: COVER IMAGE Source Type: research

Removal of jitter noise in 3D shape recovery from image focus by using Kalman filter
Abstract In regard to Shape from Focus, one critical factor impacting system application is mechanical vibration of the translational stage causing jitter noise along the optical axis. This noise is not detectable by simply observing the image. However, when focus measures are applied, inaccuracies in the depth occur. In this article, jitter noise and focus curves are modeled by Gaussian distribution and quadratic function, respectively. Then Kalman filter is designed and applied to eliminate this noise in the focus curves, as a post‐processing step after the focus measure application. Experiments are implemented with si...
Source: Microscopy Research and Technique - November 8, 2017 Category: Laboratory Medicine Authors: Hoon ‐Seok Jang, Mannan Saeed Muhammad, Tae‐Sun Choi Tags: MRT LETTER Source Type: research

A novel classification scheme to decline the mortality rate among women due to breast tumor
Abstract Early screening of skeptical masses or breast carcinomas in mammograms is supposed to decline the mortality rate among women. This amount can be decreased more on development of the computer‐aided diagnosis with reduction of false suppositions in medical informatics. Our aim is to provide a robust tumor detection system for accurate classification of breast masses using normal, abnormal, benign, or malignant classes. The breast carcinomas are classified on the basis of observed classes. This is highly dependent on feature extraction process. In propose work, a novel algorithm for classification based on the comb...
Source: Microscopy Research and Technique - November 1, 2017 Category: Laboratory Medicine Authors: Bushra Mughal, Muhammad Sharif, Nazeer Muhammad, Tanzila Saba Tags: RESEARCH ARTICLE Source Type: research

ATR ‐FTIR spectroscopy and μ‐EDXRF spectrometry monitoring of enamel erosion caused by medicaments used in the treatment of respiratory diseases
In this study, we evaluated the erosive capacity of four most commonly prescribed respiratory disease syrup medicaments on enamel using micro‐energy‐dispersive X‐ray fluorescence spectrometry (µ‐EDXRF) and attenuated total reflection Fourier transform infrared spectroscopy (ATR‐FTIR). Fifty‐five enamel fragments obtained from 30 bovine teeth were treated with artificial saliva (S), acebrofilin hydrochloride (AC), ambroxol hydrochloride (AM), bromhexine hydrochloride (BR), and salbutamol sulfate (SS); by immersing in 3 mL of respective solutions for 1 min, three times a day at intervals of 1 hr, for 5 days. µ...
Source: Microscopy Research and Technique - November 1, 2017 Category: Laboratory Medicine Authors: Raimundo Nonato Silva Gomes, Tanmoy T. Bhattacharjee, Luis Felipe C. S. Carvalho, Lu ís Eduardo Silva Soares Tags: RESEARCH ARTICLE Source Type: research

Airyscan super ‐resolution microscopy of mitochondrial morphology and dynamics in living tumor cells
Abstract Mitochondrial morphology is regulated by continuous fusion‐and‐fission events that are essential for maintaining normal function. Despite the prominence of mitochondrial function in energy generation and cell signaling, understanding of processes of fusion and fission dynamics has been hampered by the lack of high‐resolution optical systems that accommodate live‐cell imaging. We have examined different confocal modalities in terms of resolution and signal‐to‐noise ratio (SNR) in a point scanning confocal microscope with Airyscan super‐resolution (AS‐SR). Results indicated that Airyscan (AS) provide...
Source: Microscopy Research and Technique - November 1, 2017 Category: Laboratory Medicine Authors: Vladimir L. Kolossov, Mayandi Sivaguru, Joseph Huff, Katherine Luby, Kaviamuthan Kanakaraju, H. Rex Gaskins Tags: RESEARCH ARTICLE Source Type: research

Effect of novel chelating agents on the push ‐out bond strength of calcium silicate cements to the simulated root‐end cavities
Abstract To compare the effects of different chelating agents on the push‐out bond strength of calcium silicate‐based cements to the simulated root‐end cavities. Root‐end cavities were prepared on the roots of fifty extracted maxillary anterior teeth. The specimens were then randomly divided into 5 groups (n = 10) based on the final irrigation regimen: Group 1: 17% EDTA, Group 2: 7% maleic acid, Group 3: QMix, Group 4: 2.25% peracetic acid (PAA), Group 5: 0.9% saline. Then, the samples from each group were subdivided into two groups (n = 5) based on the apical filling material. In group 1, root‐end caviti...
Source: Microscopy Research and Technique - November 1, 2017 Category: Laboratory Medicine Authors: Nidambur Vasudev Ballal, Özgür İlke Ulusoy, Shubha Chhaparwal, Kishore Ginjupalli Tags: RESEARCH ARTICLE Source Type: research

Ultramorphological and molecular characteristics of the larval stages of the horse nasal ‐myiasis fly, Rhinoestrus sp. (Diptera: Oestridae) from Mongolian horse in China
This study provides a molecular characterization of Rhinoestrus sp. from Mongolian horse, an obligatory myiasis‐producing species, and expands the present knowledge of the ultramorphological characteristics of L1–L3 larvae. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - November 1, 2017 Category: Laboratory Medicine Authors: Junbin Dong, Haiquan Bao, Lai Mang Tags: RESEARCH ARTICLE Source Type: research

Polymerization shrinkage and porosity profile of dual cure dental resin cements with different adhesion to dentin mechanisms
Abstract This research aims to probe the porosity profile and polymerization shrinkage of two different dual cure resin cements with different dentin bonding systems. The self‐adhesive resin cement RelyX U200 (named RU) and the conventional Allcem Core (named AC) were analyzed by x‐ray microtomography (μCT) and Scanning Electron Microscopy (SEM). Each cement was divided into two groups (n = 5): dual‐cured (RUD and ACD) and self‐cured (RUC and ACC). μCT demonstrated that the method of polymerization does not influence the porosity profile but the polymerization shrinkage. Fewer concentration of pores was obser...
Source: Microscopy Research and Technique - October 30, 2017 Category: Laboratory Medicine Authors: Adrieli Burey, Paulo Jos é dos Reis, Bruno Luiz Santana Vicentin, Cássia Cilene Dezan Garbelini, Márcio Grama Hoeppner, Carlos Roberto Appoloni Tags: RESEARCH ARTICLE Source Type: research

Effects of EDTA gel and chlorhexidine gel on root dentin permeability
Abstract The aim of this study was to evaluate the effect of 24% ethylenediaminetetraacetic acid (EDTA) gel and 2% chlorhexidine gel (CHX) in dentin permeability and smear layer removal from root canals instrumented with NiTi rotary system using histochemical staining and scanning electron microscopy (SEM) analysis. Overall, 43 premolars were classified into two experimental groups, EDTA (n = 20) and CHX (n = 20), and a negative control (NC) (n = 3). All specimens were instrumented and the irrigant solutions were used after each file change. The EDTA group received a final rinse with 5‐ml 1% NaOCl followed by...
Source: Microscopy Research and Technique - October 30, 2017 Category: Laboratory Medicine Authors: Rayana Longo Bighetti Trevisan, Renata Siqueira Scatolin, Larissa Moreira Spinola de Castro Raucci, Walter Raucci Neto, Izabel Cristina Froner Tags: RESEARCH ARTICLE Source Type: research

Natural enamel caries in quinoline: Volumetric data and the pattern of infiltration
In conclusion, αd was the main predictor of quinoline infiltration, but it differed from the water volume with a small effect size, and the pore volume fraction with quinoline was much lower than previous estimates. Volume of oil infiltrated in enamel caries is presented for histological zones relevant for progress and reversal of caries. Oil infiltrated < 50% of the enamel pore volume and it is well correlated with permeability but not with mineral content. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - October 30, 2017 Category: Laboratory Medicine Authors: Camila Santos de Mattos Brito, K ássia Regina Simões Meira, Frederico Barbosa de Sousa Tags: RESEARCH ARTICLE Source Type: research

Jansen ‐MIDAS: A multi‐level photomicrograph segmentation software based on isotropic undecimated wavelets
Abstract Image segmentation, the process of separating the elements within a picture, is frequently used for obtaining information from photomicrographs. Segmentation methods should be used with reservations, since incorrect results can mislead when interpreting regions of interest (ROI). This decreases the success rate of extra procedures. Multi‐Level Starlet Segmentation (MLSS) and Multi‐Level Starlet Optimal Segmentation (MLSOS) were developed to be an alternative for general segmentation tools. These methods gave rise to Jansen‐MIDAS, an open‐source software. A scientist can use it to obtain several segmentatio...
Source: Microscopy Research and Technique - October 20, 2017 Category: Laboratory Medicine Authors: Alexandre Fioravante de Siqueira, Fl ávio Camargo Cabrera, Wagner Massayuki Nakasuga, Aylton Pagamisse, Aldo Eloizo Job Tags: RESEARCH ARTICLE Source Type: research

Issue Information
(Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - October 19, 2017 Category: Laboratory Medicine Tags: ISSUE INFORMATION Source Type: research

Cover Image
On the cover: The cover image, by Anna Michalik et al., is based on the Research Article The structure and ultrastructure of the egg capsules of stoneflies of the genus Isoperla (Insecta, Plecoptera, Perlodidae), DOI: 10.1002/jemt.22922. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - October 19, 2017 Category: Laboratory Medicine Authors: Anna Michalik, Marko Mili ša, Katarzyna Michalik, Elżbieta Rościszewska Tags: COVER IMAGE Source Type: research

Adhesion, unfolding forces, and molecular elasticity of fibronectin coatings: An atomic force microscopy study
In this study we have investigated the adhesion and mechanical properties of fibronectin coatings. The coatings were also used to study the role of the residence time and the influence of the loading rate in nonspecific interactions. The results showed that the adhesion force between silica and fibronectin increased with loading rate delivering similar values for residence times of 1 and 2 s. Further analysis indicated that the distance to the transition state was about 0.5 nm. Moreover, the adhesion force did not vary with the loading rate for contact time of 0 s. The unfolding of fibronectin domains also depended of the ...
Source: Microscopy Research and Technique - October 7, 2017 Category: Laboratory Medicine Authors: Maria Sumarokova, Jagoba Iturri, Jos é L. Toca‐Herrera Tags: RESEARCH ARTICLE Source Type: research