Technical implementations of light sheet microscopy
We present an overview of recent technological advances in light‐sheet fluorescence microscopy (LSFM). The focus will be on custom‐built set‐ups as well as commercially available LSFM microscopes. Special attention goes to designs that allow integration of LSFM on standard microscope bodies. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 11, 2018 Category: Laboratory Medicine Authors: Zagato Elisa, Brans Toon, Stefaan C. De Smedt, Remaut Katrien, Neyts Kristiaan, Braeckmans Kevin Tags: REVIEW ARTICLE Source Type: research
Two ‐frequency CARS imaging by switching fiber laser excitation
We describe a method for switching between two vibrational frequencies based on a single fiber‐laser source. Stokes pulses were generated by soliton self‐frequency shifting in a photonic crystal fiber. Pump and Stokes pulses were stretched to enhance vibrational resolution by spectral focusing. Stokes pulses were switched between two wavelengths on the millisecond time scale by a liquid‐crystal retarder. Proof‐of‐principle is demonstrated by coherent anti‐Stokes Raman imaging of polystyrene beads embedded in a poly(methyl methacrylate) (PMMA) matrix. The Stokes shift was switched between 3,050 cm−1, where pol...
Source: Microscopy Research and Technique - January 11, 2018 Category: Laboratory Medicine Authors: Eric C. Rentchler, Ruxin Xie, Rongqing Hui, Carey K. Johnson Tags: MRT LETTER Source Type: research
Growth rings in roots of medicinal perennial dicotyledonous herbs from temperate and subtropical zones in China
Abstract
Growth years are closely related to the quality of Chinese medicinal materials, and they can provide a reliable basis for quality evaluation on crude drugs in accordance with the suitable growth years. Herbchronology, the numerical and pattern analysis of annual growth rings in herbaceous perennials, has emerged to be a recognized science that is applied in age determination of perennial forbs. Fifty medicinal species growing in temperate and subtropical zones of China were examined by microstructure observation to determine the presence of growth rings in the secondary root xylem of medicinal perennial dicotyledo...
Source: Microscopy Research and Technique - January 11, 2018 Category: Laboratory Medicine Authors: Shanshan Chu, Lingling Tan, Chanchan Liu, Huasheng Peng, Haiyan Duan, Luqi Huang Tags: RESEARCH ARTICLE Source Type: research
Observation of curative effect of intravitreal injection of conbercept in wet age ‐related macular degeneration: Optical coherence tomography analysis after injection
Microscopy Research and Technique,Volume 81, Issue 4, Page 384-388, April 2018. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 10, 2018 Category: Laboratory Medicine Source Type: research
Pigmented macrophages and related aggregates in the spleen of european sea bass dosed with heavy metals: Ultrastructure and explorative morphometric analysis
Microscopy Research and Technique,Volume 81, Issue 4, Page 351-364, April 2018. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 10, 2018 Category: Laboratory Medicine Source Type: research
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Microscopy Research and Technique, Ahead of Print. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 10, 2018 Category: Laboratory Medicine Source Type: research
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Microscopy Research and Technique,Volume 81, Issue 3, Page 338-347, March 2018. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 10, 2018 Category: Laboratory Medicine Source Type: research
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Microscopy Research and Technique,Volume 81, Issue 3, Page 315-331, March 2018. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 10, 2018 Category: Laboratory Medicine Source Type: research
Pigmented macrophages and related aggregates in the spleen of european sea bass dosed with heavy metals: Ultrastructure and explorative morphometric analysis
Abstract
The ultrastructure and morphometrics of pigmented macrophages (PMs) were assessed in the spleen of European sea bass experimentally dosed with Cd and Hg. PMs occurred either as solitary cells or as variably structured aggregations, defined as macrophage aggregates (MAs). Light microscopy revealed a high degree of morphological heterogeneity amongst MAs of all experimental groups. At the ultrastructural level, MAs showed a heterogeneous pigment content that was not influenced by the treatment. Cytoplasm rarefaction/vacuolation and euchromatic nuclei, were observed in PMs of dosed fish. Undosed and Cd‐dosed sample...
Source: Microscopy Research and Technique - January 10, 2018 Category: Laboratory Medicine Authors: Maurizio Manera, Bahram Sayyaf Dezfuli, Joseph A. DePasquale, Luisa Giari Tags: RESEARCH ARTICLE Source Type: research
Hybrid feature extraction techniques for microscopic hepatic fibrosis classification
In conclusion, this work is of special value that provides promising results for early prediction of the liver fibrosis in schistosomiais and other fibrotic liver diseases in no time with expected better prognosis after treatment.
Staging of liver fibrosis is essential for accurate diagnosis and therapy of liver diseases.
A new hybrid combination of statistical features with empirical mode decomposition is proposed.
BPNN achieved superior accuracy compared to linear SVM, quadratic SVM, and cubic SVM (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 10, 2018 Category: Laboratory Medicine Authors: Dalia S. Ashour, Dina M. Abou Rayia, Mohamed Maher Ata, Amira S. Ashour, Mustafa M. Abd Elnaby Tags: RESEARCH ARTICLE Source Type: research
Field of view scanning based quantitative interferometric microscopic cytometers for cellular imaging and analysis
Microscopy Research and Technique,Volume 81, Issue 4, Page 397-407, April 2018. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 9, 2018 Category: Laboratory Medicine Source Type: research
