The micromorphological characterization of adhesive bond in dentin different locations
In this study, we found the difference in interfacial micromorphology in dentin different locations. Also, marginal gap was found in both observed dentin area. Better understanding of complexity and three‐ dimensional variations of the tooth structure is important for prevention of clinical challenges such as postoperative sensitivity, marginal discoloration and secondary caries, which could be prevented by achieving of predictable and long‐lasting adhesive bond.
In this article, we report on the influence of dentinal orientation on the quality of adhesive bond between composite restorations and dentin different locat...
Source: Microscopy Research and Technique - October 7, 2017 Category: Laboratory Medicine Authors: Bojana D. Ramic, Milan R. Drobac, Igor L. Stojanac, Milica T. Premovic, Ljubomir M. Petrovic Tags: RESEARCH ARTICLE Source Type: research
Testing feasibility of an accurate microscopic assessment of macrovesicular steatosis in liver allograft biopsies by smartphone add ‐on lenses
Abstract
Light microscopy is an essential tool in histological examination of tissue samples. However, the required equipment for a correct and rapid diagnosis is sometimes unavailable. Smartphones and mobile phone networks are widespread, and could be used for diagnostic imaging and telemedicine. Macrovesicular steatosis (MS) is a major risk factor for liver graft failure, and is only assessable by microscopic examination of a frozen tissue section. The aim of this study was to compare the microscopic assessment of MS in liver allograft biopsies by a smartphone with eyepiece adaptor (BLIPS device) to standard light micros...
Source: Microscopy Research and Technique - October 1, 2017 Category: Laboratory Medicine Authors: Manuela Cesaretti, Nicolas Pot é, Federica Dondero, François Cauchy, Anne Sophie Schneck, Olivier Soubrane, Valerie Paradis, Alberto Diaspro, Andrea Antonini Tags: RESEARCH ARTICLE Source Type: research
Palyno ‐morphological characteristics of gymnosperm flora of pakistan and its taxonomic implications with LM and SEM methods
Abstract
The present study is intended to assess gymnosperms pollen flora of Pakistan using Light Microscope (LM) and Scanning Electron Microscopy (SEM) for its taxonomic significance in identification of gymnosperms. Pollens of 35 gymnosperm species (12 genera and five families) were collected from its various distributional sites of gymnosperms in Pakistan. LM and SEM were used to investigate different palyno‐morphological characteristics. Five pollen types (i.e., Inaperturate, Monolete, Monoporate, Vesiculate‐bisaccate and Polyplicate) were observed. Six In equatorial view seven types of pollens were observed, in wh...
Source: Microscopy Research and Technique - October 1, 2017 Category: Laboratory Medicine Authors: Raees Khan, Sheikh Zain Ul Abidin, Mushtaq Ahmad, Muhammad Zafar, Jie Liu, Hafiza Amina Tags: RESEARCH ARTICLE Source Type: research
The effect of different thermal treatment on the physical properties of PGA/PCL copolymer suture material
Abstract
Samples of polyglycolic acid PGA/polycaprolactone PCL copolymer absorbable suture materials, which is called Poliglecaprone PGC copolymer suture, were thermally treated by two different processes. The first method is the direct heat exposure, from 20 to 38 °C, in the surrounding medium. Which is an environmental study. The other method is the annealing process at temperatures ranged from 65, 80, 100, 120, and 140 °C, for two different duration periods 60 and 120 min. This is an extensive technical study. The refractive indices of thermally treated PGA/PCL copolymer sutures were measured using multiple‐beam...
Source: Microscopy Research and Technique - October 1, 2017 Category: Laboratory Medicine Authors: K. A. El ‐Farahaty, M. A. El‐Bakary, N. M. El‐Sayed Tags: RESEARCH ARTICLE Source Type: research
Morphological characterization of ckd in cats: Insights of fibrogenesis to be recognized
Abstract
Renal fibrosis is characterized by glomerulosclerosis and tubulointerstitial fibrosis and its pathogenesis is associated with the activity of mesenchymal cells (fibroblasts), being essentially characterized by a process of excessive accumulation resulting from the deposition of extracellular matrix components. The aim of this study was to characterize the morphological presentation of chronic and fibrotic lesions in the glomerular, tubular, interstitial, and vascular compartments in feline CKD, as well as the possible participation of myofibroblasts in renal fibrotic processes in this species. Cat kidneys were col...
Source: Microscopy Research and Technique - October 1, 2017 Category: Laboratory Medicine Authors: G. B. Morais, D. A. Viana, J. M. Verdugo, M. G. Rosell ó, J. O. Porcel, D. D. Rocha, F. A. F. Xavier Júnior, K. D. S. M. Barbosa, F. M. O. Silva, G. A. C. Brito, C. M. S. Sampaio, J. S. A. M. Evangelista Tags: RESEARCH ARTICLE Source Type: research
Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy
This article studies the morphological and mechanical features of multinuclear and mononuclear SW480 colon cancer cells by atomic force microscopy to understand their drug‐resistance. The SW480 cells were incubated with the fullerenol concentrations of 1 mg/ml and 2 mg/ml. Morphological and mechanical features including the height, length, width, roughness, adhesion force and Young's modulus of three multinuclear cell groups and three mononuclear cell groups were imaged and analyzed. It was observed that the features of multinuclear cancer cells and mononuclear cancer cells were significantly different after the treatmen...
Source: Microscopy Research and Technique - October 1, 2017 Category: Laboratory Medicine Authors: Jinyun Liu, Yingmin Qu, Guoliang Wang, Xinyue Wang, Wenxiao Zhang, Jingmei Li, Zuobin Wang, Dayou Li, Jinlan Jiang Tags: RESEARCH ARTICLE Source Type: research
Computer ‐aided diagnosis software for vulvovaginal candidiasis detection from Pap smear images
This article presents a computer aided diagnosis (CAD) software to improve human diagnosis of VVC from Pap smear samples. The proposed software is designed based on phenotypic and morphology features of the Candida in Pap smear sample images. This software provide a user‐friendly interface which consists of a set of image processing tools and analytical results that helps to detect Candida and determine severity of illness. The software was evaluated on 200 Pap smear sample images and obtained specificity of 91.04% and sensitivity of 92.48% to detect VVC. As a result, the use of the proposed software reduces diagnostic t...
Source: Microscopy Research and Technique - October 1, 2017 Category: Laboratory Medicine Authors: Mohammadreza Momenzadeh, Alireza Vard, Ardeshir Talebi, Alireza Mehri Dehnavi, Hossein Rabbani Tags: RESEARCH ARTICLE Source Type: research
Application of autofluorescence robotic histology for quantitative evaluation of the 3 ‐dimensional morphology of murine articular cartilage
This study aimed to develop a novel imaging method for 3‐dimensional (3D) histology of mouse articular cartilage, using a robotic system—termed here “3D histocutter”—which automatically sections tissue samples and serially acquires fluorescence microscopy images of each section. Tibiae dissected from C57Bl/6 mice, either naïve or OA‐induced by surgical destabilization of the medial meniscus (DMM), were imaged using the 3D histocutter by exploiting tissue autofluorescence. Accuracy of 3D imaging was validated by ex vivo contrast‐enhanced micro‐CT and sensitivity to lesion detection compared with conventiona...
Source: Microscopy Research and Technique - September 30, 2017 Category: Laboratory Medicine Authors: Patricia Das Neves Borges, Tonia L. Vincent, Massimo Marenzana Tags: RESEARCH ARTICLE Source Type: research
Issue Information
(Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - September 25, 2017 Category: Laboratory Medicine Tags: ISSUE INFORMATION Source Type: research
Cover Image
On the cover: The cover image, by Alejandro del Palacio et al., is based on the Research Article A new method using Scanning Electron Microscopy (SEM) for preparation of anisopterous odonates, DOI: 10.1002/jemt.22903. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - September 25, 2017 Category: Laboratory Medicine Authors: Alejandro del Palacio, Patricia Laura Sarmiento, Muz ón Javier Tags: COVER IMAGE Source Type: research
Bones Morphogenic Protein ‐4 and retinoic acid combined treatment comparative analysis for in vitro differentiation potential of murine mesenchymal stem cells derived from bone marrow and adipose tissue into germ cells
Abstract
Nowadays, infertility is no longer considered as an unsolvable disorder due to progresses in germ cells derived from stem lineage with diverse origins. Technical and ethical challenges push researchers to investigate various tissue sources to approach more efficient gametes. The purpose of the current study is to investigate the efficacy of a combined medium, retinoic acid (RA) together with Bone Morphogenic Protein‐4 (BMP4), on differentiation of Bone Marrow Mesenchymal Stem Cells (BMMSCs) and adipose‐derived mesenchymal stem cells (ADMSCs) into germ cells. Murine MSCs were obtained from both Bone Marrow (BM)...
Source: Microscopy Research and Technique - September 18, 2017 Category: Laboratory Medicine Authors: Maryam H. Shirzeily, Neda Khanlarkhani, Fardin Amidi, Farshad H. Shirzeily, Fereydoon S. Aval, Aligholi Sobhani Tags: ORIGINAL ARTICLE Source Type: research
Evaluation of the effect of four final irrigation protocols on root canal dentin components by polarized light microscopy and scanning electron microscopy
Abstract
The present study aimed to evaluate the effects of four endodontic chelating agents, followed by 2.5% sodium hypochlorite (NaOCl), as final irrigation regimens on organic and inorganic components of human root dentin. Sixty mandibular incisors were prepared and randomly divided into six groups (n = 10): QMiX, 1% peracetic acid (PA), 17% EDTA, 10% citric acid (CA), 2.5% NaOCl (solution control) and distilled water (DW‐negative control). After irrigation with the chelating agents, a final flush was performed with 2.5% NaOCl. The specimens were split longitudinally in halves; one was designated for organic comp...
Source: Microscopy Research and Technique - September 1, 2017 Category: Laboratory Medicine Authors: Fl ávia Emi Razera Baldasso, Luana Roleto Cardoso, Vinicius Duval da Silva, Renata Dornelles Morgental, Patrícia Maria Poli Kopper Tags: RESEARCH ARTICLE Source Type: research
Characterization of lacquer films from the middle and late Chinese warring states period 476 –221BC
Abstract
The famous lacquer wares excavated from the Jiuliandun Tombs of the middle and late Warring States period (476–221 BC) were analyzed using scanning electron microscopy/energy as well as dispersive X‐ray spectrometry (SEM/EDS), Raman spectroscopy (RS), optical microscopy (OM), and X‐ray diffraction (XRD). The results showed a multilayer structure in the lacquer film, including a Qihui layer (a layer made of lacquer and various plasters), undercoat layer (or finishing coat) and colored paint layer mixed with various inorganic particles, such as quartz (SiO2) and hydroxyapatite [Ca5(PO4)3(OH)], as fillers in th...
Source: Microscopy Research and Technique - September 1, 2017 Category: Laboratory Medicine Authors: P.J. Jin, Y.L. Hu, Z.B. Ke Tags: RESEARCH ARTICLE Source Type: research
Influence of scanning rate on quality of AFM image: Study of surface statistical metrics
Abstract
The purpose of this work is to study the dependence of AFM‐data reliability on scanning rate. The three‐dimensional (3D) surface topography of the samples with different micro‐motifs is investigated. The analysis of surface metrics for estimation of artifacts from inappropriate scanning rate is presented. Fractal analysis was done by cube counting method and evaluation of statistical metrics was carrying out on the basis of AFM‐data. Combination of quantitate parameters is also presented in graphs for every measurement. The results indicate that the sensitivity to scanning rate growths with fractal dimensi...
Source: Microscopy Research and Technique - September 1, 2017 Category: Laboratory Medicine Authors: Dinara Sobola, Ştefan Ţălu, Shahram Solaymani, Lubomír Grmela Tags: RESEARCH ARTICLE Source Type: research
Site ‐specific accumulation and dynamic change of flavonoids in Apocyni Veneti Folium
Abstract
Site‐specific accumulation of flavonoids in Apocyni Veneti Folium was determined by laser scanning confocal microscope (LSCM) and the localization of catechins also was observed via vanillin‐HCl staining under the conventional optical microscope. The contents of five flavonoids in Apocyni Veneti Folium from different harvest times and growth parts were measured using HPLC method. LSCM observation showed that flavonoids are accumulated in cuticle of epidermal cells and vessel walls, especially in protoplasts and nucleolus of the collenchyma cells and the epidermal cells. Catechins are localized in the palisade ...
Source: Microscopy Research and Technique - September 1, 2017 Category: Laboratory Medicine Authors: Cui ‐Hua Chen, Hu Xu, Xun‐Hong Liu, Li‐Si Zou, Mei Wang, Zi‐Xiu Liu, Xing‐Sheng Fu, Hui Zhao, Ying Yan Tags: RESEARCH ARTICLE Source Type: research
