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Microscopy Research and Technique, Ahead of Print. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 9, 2018 Category: Laboratory Medicine Source Type: research
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Microscopy Research and Technique,Volume 81, Issue 3, Page 259-266, March 2018. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - January 8, 2018 Category: Laboratory Medicine Source Type: research
Microscopic abnormality classification of cardiac murmurs using ANFIS and HMM
Abstract
Auscultation of heart dispenses identification of the cardiac valves. An electronic stethoscope is used for the acquisition of heart murmurs that is further classified into normal or abnormal murmurs. The process of heart sound segmentation involves discrete wavelet transform to obtain individual components of the heart signal and its separation into systole and diastole intervals. This research presents a novel scheme to develop a semi‐automatic cardiac valve disorder diagnosis system. Accordingly, features are extracted using wavelet transform and spectral analysis of input signals. The proposed classification...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: H. M. Fahad, M. Usman Ghani Khan, Tanzila Saba, Amjad Rehman, Sajid Iqbal Tags: RESEARCH ARTICLE Source Type: research
Brain tumor segmentation in multi ‐spectral MRI using convolutional neural networks (CNN)
This article presents a deep convolutional neural network (CNN) to segment brain tumors in MRIs. The proposed network uses BRATS segmentation challenge dataset which is composed of images obtained through four different modalities. Accordingly, we present an extended version of existing network to solve segmentation problem. The network architecture consists of multiple neural network layers connected in sequential order with the feeding of Convolutional feature maps at the peer level. Experimental results on BRATS 2015 benchmark data thus show the usability of the proposed approach and its superiority over the other appro...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: Sajid Iqbal, M. Usman Ghani, Tanzila Saba, Amjad Rehman Tags: RESEARCH ARTICLE Source Type: research
Morphological specializations of the epidermis of an angler catfish Chaca chaca (Siluriformes, Chacidae) in relation to its ecological niche: A scanning electron microscopic investigation
Abstract
The present work was undertaken with the aim to deduce morphological adaptations in skin of an angler catfish Chaca chaca by means of scanning electron microscopy. The fish is nocturnal, bottom dwelling, sluggish, ambush predator, lives in sand, mud, or soft substrates often buried and camouflaged for protection and to feed. The surface of the epidermis is covered with polygonal epithelial cells, each having surface relief of microridges forming intricate patterns. In between epithelial cells irregularly distributed mucous cell openings, randomly distributed epidermal specialized structures, taste buds, and neurom...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: Arup Mistri, Usha Kumari, Swati Mittal, Ajay Kumar Mittal Tags: RESEARCH ARTICLE Source Type: research
Dentin bond strength and nanoleakage of the adhesive interface after intracoronal bleaching
This study evaluated dentin bond strength (BS) and nanoleakage of non‐ and pre‐etched dentin immediately (T0,), 7 days (T7), and 14 days (T14) after bleaching. Bovine incisors (150) were selected and half of them submitted to intrapulpal dentin etching (e). Non‐ and pre‐etched dentin were subjected to the following (n = 15): no bleaching/control (C); 35% carbamide peroxide (CP); 35% hydrogen peroxide (35% HP); 25% hydrogen peroxide (25% HP); and sodium perborate (SP). Bleaching agents were applied to the pulp chamber four times within a 72‐h interval. Afterwards, pulp chamber dentin was prepared for the BS te...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: Vanessa Cavalli, Maicon Sebold, Mirela Sanae Shinohara, Patr ícia Nóbrega Rodrigues Pereira, Marcelo Giannini Tags: RESEARCH ARTICLE Source Type: research
Effect of ethanol on the antimicrobial properties of chlorhexidine over oral biofilm
Abstract
The aim of this study was to evaluate the effect of 95% ethanol irrigation, with 5 or 10 min of action, on the antibacterial properties of 2% chlorhexidine (CHX), on oral biofilm, evaluated with confocal laser scanning microscopy (CLSM). Oral biofilm development was induced in 80 sterilized bovine dentin blocks, distributed in two groups (5 or 10 min) and 4 subgroups, according to time and the solution used: Saline (SALINE5, SALINE10); Saline followed by CHX (SALINE/CHX5, SALINE/CHX10); Ethanol (ETHANOL5, ETHANOL10), Ethanol followed by CHX (ETHANOL/CHX5, ETHANOL/CHX10). The surface of the block was dyed with Live...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: Pedro Henrique Marks Duarte, Paula Barcellos da Silva, Ricardo Abreu da Rosa, Francisco Montagner, Marco Ant ônio Húngaro Duarte, Milton Carlos Kuga, Marcus Vinícius Reis Só Tags: RESEARCH ARTICLE Source Type: research
Effects of fixation on electrophysiology and structure of human jejunal villi
Abstract
The villi of human jejunum vary in size and shape during different functional conditions. In the base the lamina propria is isotonic with blood, in the tip hyperosmotic. Here we study electrophysiological and morphological effects of incubation in hypotonic, isotonic, or hypertonic solutions, and to test various isotonic fixatives for microscopy. Samples of jejunal mucosae, obtained during surgery in obese patients, were studied in Ussing chambers where electrical parameters were registered during incubation in Krebs solution at various osmolarities, and during fixation in formaldehyde, glutaraldehyde, or osmium t...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: Anna Casselbrant, Herbert F. Helander Tags: RESEARCH ARTICLE Source Type: research
Observation of curative effect of intravitreal injection of conbercept in wet age ‐related macular degeneration: Optical coherence tomography analysis after injection
Abstract
To observe the clinical efficacy of intravitreal injection of conbercept in the treatment of wet age‐related macular degeneration (wAMD), optical coherence tomography (OCT) and the best corrected visual acuity (BCVA) was observed to measure the changes of anatomical changes of central macular thickness (CMT) and the area and volume of retinal pigment epithelium (RPE) uplift. Fifteen patients (15 eyes) with wet AMD were enrolled in this study. All patients underwent intravitreal injection of conbercept of 0.05 mL once. After 1 week, 1 month, and 3 months, OCT and BCVA were used to examine and to compare with the ...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: Wen Yang, Ying Tan, Chaowei Li, Yi Liu, Guohua Lu Tags: RESEARCH ARTICLE Source Type: research
Tissue distribution of some immune cells in bovine reproductive tract during follicular and luteal phase
In conclusion, we did not found significant changes in immune cells according to follicular and luteal phases, but localization and numbers in each organ have changed according to both organ and layers. These results indicate that these factors may play a crucial role not only to generate an immune response but also to have a role in regulation of physiological functions in female reproductive organs.
This study was to determine the distribution of immune cells in bovine reproductive tract during sexual cycle that is considered to affect reproductive system. Immune cell localization and numbers in each organ have changed ...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: Mehmet Erdem Akbalik, Narin Liman, Hakan Sagsoz, Berna Guney Saruhan Tags: RESEARCH ARTICLE Source Type: research
Field of view scanning based quantitative interferometric microscopic cytometers for cellular imaging and analysis
Abstract
Microimaging is of great significance in the biological and medical fields, since it can realize observations acting as important references for cellular research and disease diagnosis. However, traditional microscopy only offers qualitative sample contours; moreover, it is difficult to reach large‐amount sample observations limited by the fixed field of view (FoV). To realize massive cellular measurements quantitatively, three designed quantitative interferometric microscopic cytometers based on the FoV scanning are introduced and compared in details in this article. These devices not only retrieve the quantita...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: Keding Yan, Liang Xue, Shouyu Wang Tags: RESEARCH ARTICLE Source Type: research
Physiological significance of gravity receptors on larval cephalic cuticle in the silk moth, Antheraea proylei Jolly
Abstract
The lateral aspects of larval cephalic cuticle of oak tasar moth, Antheraea proylei, a hybrid between Antheraea pernyi and Antheraea roylei exhibited the presence of gravity receptors in the form of dorsal campaniform sensilla. The distribution pattern and number of dome shaped dorsal campaniform sensilla were found to vary in different larval stages. In the first and second larval stages, 4‐5 sensilla were localized near the apex of the lateral aspects of the cephalic cuticle on either side of the head. From the third larval stage onwards, on the other hand, both the right and left lateral aspects of the cephal...
Source: Microscopy Research and Technique - January 1, 2018 Category: Laboratory Medicine Authors: Sudip Dey, S. Choudhury Tags: RESEARCH ARTICLE Source Type: research
A cone ‐beam computed tomography study of the anatomical relationships between mandibular teeth and the mandibular canal, with a review of the current literature
Abstract
The purpose of this study was to evaluate age‐ and sex‐related changes in the anatomical relationships between the roots of the molars and premolars and the mandibular canal using cone‐beam computed tomography (CBCT) images. A total of 243 patients (116 males, 127 females) aged 16–83 years for whom previous CBCT scans were available were enrolled in this study. The patients were subcategorized by sex and age (Group I, <21 years; Group II, 21–40 years; and Group III, >40 years). The distances between the mandibular canal and the molars and premolars were measured. The mandibular canal was significan...
Source: Microscopy Research and Technique - December 29, 2017 Category: Laboratory Medicine Authors: Umut Aksoy, Se çil Aksoy, Kaan Orhan Tags: RESEARCH ARTICLE Source Type: research
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Microscopy Research and Technique,Volume 81, Issue 3, Page 308-314, March 2018. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - December 29, 2017 Category: Laboratory Medicine Source Type: research
Intraspecific variation in seed morphology of tribe vicieae (Papilionoidae) using scanning electron microscopy techniques
This study is focused on seed morphological and ultrastructural aspects in five genera and 12 species of tribe Vicieae.
The seeds were characterized by oval to round shape, ovate or oblong outline, striate to papillate ornamentation, and wavy cell margin.
Two distinctive cell patterns were observed; radiate and wavy, the testa cells in Vicia sativa superficially seem to form peltate hairs. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - December 27, 2017 Category: Laboratory Medicine Authors: Neelam Rashid, Muhammad Zafar, Mushtaq Ahmad, Khafsa Malik, Ihsan ‐ul Haq, Syed Nasar Shah, Abdul Mateen, Tauseef Ahmed Tags: RESEARCH ARTICLE Source Type: research
