Effect of different implant abutment surfaces on OBA ‐09 epithelial cell adhesion
Abstract For the long‐term success of implants, it is necessary to achieve a direct contact between the implant and the subjacent bone. To avoid bacterial penetration that could adversely affect the initial wound healing as well as the long‐term behavior of the implants, an early tissue barrier must form that is able to protect the biological peri‐implant structures. Given the need of an effective tissue early barrier around dental implants, the present study evaluated, in vitro, the influence of physical and chemical characteristics of two implant abutment surfaces on gingival epithelial cells (OBA‐9) adhesion. To...
Source: Microscopy Research and Technique - August 30, 2017 Category: Laboratory Medicine Authors: Maria S ílvia M. Rigolin, Erica Dorigatti de Avila, Fernanda G. Basso, Josimeri Hebling, Carlos Alberto de S. Costa, Francisco de Assis Mollo Junior Tags: MRT LETTER Source Type: research

Efficacy of different irrigant protocols and application systems on sealer penetration using a stepwise CLSM analysis
This study compared several irrigation protocols and application systems for sealer penetration into dentinal tubules. Single‐rooted‐human teeth were divided into 5 experimental groups (n = 15) and a control group (n = 5), according to final irrigation protocols: standard needle irrigation (SNI); Vibringe; Vibringe + NaviTip FX (Vibringe NFX); Endo Spray (ES); and passive‐ultrasonic‐irrigation (PUI). Following obturation of the root canals, the percentage of the sealer penetration was measured at different depths using stepwise CLSM analysis. The sealer penetration in the experimental groups was signifi...
Source: Microscopy Research and Technique - August 28, 2017 Category: Laboratory Medicine Authors: Hacer Aksel, Selen K üçükkaya Eren, Nuhan Puralı, Ahmet Serper, Adham A. Azim Tags: RESEARCH ARTICLE Source Type: research

Honeysuckle flowers extract loaded Bombyx mori silk fibroin films for inducing apoptosis of HeLa cells
This study aimed to prepare silk fibroin (SF) films loaded with honeysuckle flowers extract (HFE) for inducing apoptosis of HeLa cells. We mixed solution of SF and HFE by air‐drying for preparing the honeysuckle flowers extract loaded silk fibroin (SFH) films. The physical properties including morphologies, contact angle, roughness, and Z range were characterized. MTS assay and fluorescence micrographs proved that SFH films inhibited the proliferation rate of HeLa cells due to induction of HFE into SF films. Furthermore, cell apoptosis assay and cell cycle analysis confirmed that the apoptosis of HeLa cells resulted from...
Source: Microscopy Research and Technique - August 26, 2017 Category: Laboratory Medicine Authors: Chenlin Li, Mingying Yang, Liangjun Zhu, Yongqiang Zhu Tags: RESEARCH ARTICLE Source Type: research

The influence of different skin types on GSR sampling by tape lifting for SEM analysis
In this study, the change in the amounts the gunshot residues (GSR) collected by the “swab” method from shooter's right hand was analyzed using Scanning Electron Microscope‐Energy Dispersive Spectroscopy (SEM‐EDS) to test dependency on various conditions (such as skin color of the shooter, various physical properties, hair density of hands, size of the hands, sweaty or creamy (oiliness) hands). As a result of the analysis, there was no significant difference in the amount of GSR according to skin color, however, there was a change in the amount of GSR depending on the physical characteristics of the shooter. These ...
Source: Microscopy Research and Technique - August 25, 2017 Category: Laboratory Medicine Authors: İlker Kara Tags: RESEARCH ARTICLE Source Type: research

Morphostructural and immunohistochemical study on the role of metallothionein in the detoxification of heavy metals in Apis mellifera L., 1758
Abstract Honey is a valuable food produced by bees from sugary substances that they gather in nature. The transformation the nectar into honey, by bees, is long and complex. Except for honey, where heavy metals are absent or are found only in traces, the bees and their products have always been considered excellent biomarkers of such contaminants. We have assumed that the absence of heavy metals in honey is due to the presence of a detoxification system in the digestive system of bees, which involves metallothioneins, proteins that have a role in the homeostatic control of essential and non‐essential metals. We have plac...
Source: Microscopy Research and Technique - August 22, 2017 Category: Laboratory Medicine Authors: Antonio Salvaggio, Roberta Pecoraro, Elena Maria Scalisi, Daniele Tibullo, Bianca Maria Lombardo, Giuseppina Messina, Francesco Loreto, Chiara Copat, Margherita Ferrante, Roberto Avola, Giusi D'amante, Claudia Genovese, Salvatore Antonino Raccuia, Maria V Tags: RESEARCH ARTICLE Source Type: research