Field of view scanning based quantitative interferometric microscopic cytometers for cellular imaging and analysis

Abstract Microimaging is of great significance in the biological and medical fields, since it can realize observations acting as important references for cellular research and disease diagnosis. However, traditional microscopy only offers qualitative sample contours; moreover, it is difficult to reach large‐amount sample observations limited by the fixed field of view (FoV). To realize massive cellular measurements quantitatively, three designed quantitative interferometric microscopic cytometers based on the FoV scanning are introduced and compared in details in this article. These devices not only retrieve the quantitative sample phase distributions in the extended FoV, but also provide the detailed information of massive cells, such as cellular volume, area, and roundness. Considering their capabilities as quantitative imaging and large‐amount sampling, it is believed that these quantitative interferometric microscopic cytometers (QIMCs) can be potentially adopted in high‐throughput cell imaging and statistical analysis for both the biological and medical applications. Use of the FoV scanning based QIMCs, not only the cellular quantitative phase imaging can be retrieved, but also the details of massive cells, including cellular volume, area, and roundness, are accurately extracted. Quantitative interferometric microscopic cytometers are proposed for cellular phase imaging and configuration extraction. Field of view scanning is adopted to increase the cellular amoun...
Source: Microscopy Research and Technique - Category: Laboratory Medicine Authors: Tags: RESEARCH ARTICLE Source Type: research