ATR ‐FTIR spectroscopy and μ‐EDXRF spectrometry monitoring of enamel erosion caused by medicaments used in the treatment of respiratory diseases

In this study, we evaluated the erosive capacity of four most commonly prescribed respiratory disease syrup medicaments on enamel using micro‐energy‐dispersive X‐ray fluorescence spectrometry (µ‐EDXRF) and attenuated total reflection Fourier transform infrared spectroscopy (ATR‐FTIR). Fifty‐five enamel fragments obtained from 30 bovine teeth were treated with artificial saliva (S), acebrofilin hydrochloride (AC), ambroxol hydrochloride (AM), bromhexine hydrochloride (BR), and salbutamol sulfate (SS); by immersing in 3 mL of respective solutions for 1 min, three times a day at intervals of 1 hr, for 5 days. µ‐EDXRF analysis of enamel surface did not reveal significant erosion caused by the medications. However, ATR‐FTIR showed a detectable shift in the phosphate (PO4) antisymmetric stretching mode (ν3) at ∼985 cm−1 for AM, BR, and SS, indicating erosion. Multivariate statistical analysis showed that AC, AM, SS, and BR could be classified with 70%, 80%, 100%, and 100% efficiency from S (control), further highlighting the ability of ATR‐FTIR to identify degree of erosion. This suggests ATR‐FTIR may be used to rapidly and nondestructively investigate erosive effects of medicaments. Molecular scale monitor of the in vitro enamel erosion was obtained. A detectible shift in the phosphate band after demineralization was found. Multivariate statistical analysis allows a prompt monitoring of demineralization.
Source: Microscopy Research and Technique - Category: Laboratory Medicine Authors: Tags: RESEARCH ARTICLE Source Type: research