Biological consequences of improving the structural stability of hairpins that have antimicrobial activity
This study shows that rational design can be applied to improve the structural stability of beta‐hairpin antimicrobial peptides. Exploring the relationship between structure and activity, however, shows that antimicrobial peptide activity does not always correlate with structural stability. There is also significant evidence that structural optimization can have opposite effects on gram‐positive and gram‐negative bacteria. (Source: Journal of Peptide Science)
Source: Journal of Peptide Science - November 29, 2017 Category: Biochemistry Authors: Kalkena Sivanesam, Brandon L. Kier, Samuel D. Whedon, Champak Chatterjee, Niels H. Andersen Tags: Research Article Source Type: research
Antimicrobial activity of the indolicidin ‐derived novel synthetic peptide In‐58
Natural peptides with antimicrobial activity are extremely diverse, and peptide synthesis technologies make it possible to significantly improve their properties for specific tasks. Here, we investigate the biological properties of the natural peptide indolicidin and the indolicidin‐derived novel synthetic peptide In‐58. In‐58 was generated by replacing all tryptophan residues on phenylalanine in D‐configuration; the α‐amino group in the main chain also was modified by unsaturated fatty acid. Compared with indolicidin, In‐58 is more bactericidal, more resistant to proteinase K, and less toxic to mammalian cell...
Source: Journal of Peptide Science - November 29, 2017 Category: Biochemistry Authors: A. S. Vasilchenko, A. V. Vasilchenko, T. M. Pashkova, M. P. Smirnova, N. I. Kolodkin, I. V. Manukhov, G. B. Zavilgelsky, E. A. Sizova, O. L. Kartashova, A. S. Simbirtsev, E. A. Rogozhin, G. K. Duskaev, M. V. Sycheva Tags: Research Article Source Type: research
Issue information
(Source: Journal of Peptide Science)
Source: Journal of Peptide Science - November 29, 2017 Category: Biochemistry Tags: Issue Information Source Type: research
Structural and functional studies of differentially O ‐glycosylated analogs of a thrombin inhibitory peptide – variegin
This report, for the first time, describes the impact of differential glycosylation on the function of a thrombin inhibitory peptide and tries to provide structural insights into the relevance of peptide glycosylation in thrombin inhibition. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
Variegin is a glycoslated peptide isolated from Amblyomma variegatum. The identity of its glycosylation is unknown. Hence, four different glycosylated analogs of variegin were synthesized, and their functional and structural properties were studied. (Source: Journal of Peptide Science)
Source: Journal of Peptide Science - November 7, 2017 Category: Biochemistry Authors: Pidathala R. V. Shabareesh, Ashish Kumar, Dinakar M. Salunke, Kanwal J. Kaur Tags: Research Article Source Type: research
Synthesis, receptor binding studies, optical spectroscopic and in silico structural characterization of morphiceptin analogs with cis ‐4‐amino‐L‐proline residues
Three novel morphiceptin analogs, in which Pro in position 2 and/or 4 was replaced by cis‐4‐aminoproline connected with the preceding amino acid through the primary amino group, were synthesized. The opioid receptor affinities, functional assay results, enzymatic degradation studies and experimental and in silico structural analysis of such analogs are presented. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
Novel morphiceptin analogs, in which Pro in position 2 and/or 4 was replaced by cis‐4‐aminoproline connected with the preceding amino acid through the primary amine, were synthesiz...
Source: Journal of Peptide Science - November 7, 2017 Category: Biochemistry Authors: Anna Adamska ‐Bartłomiejczyk, Attila Borics, Csaba Tömböly, Szabolcs Dvorácskó, Marek Lisowski, Alicja Kluczyk, Grzegorz Wołczański, Justyna Piekielna‐Ciesielska, Anna Janecka Tags: Research Article Source Type: research
Design and study of lipopeptide inhibitors on preventing aggregation of human islet amyloid polypeptide residues 11 ‐20
This study demonstrated the efficiency of lipopeptides in inhibiting the aggregation of hIAPP11‐20 and proved that MST could be regarded as an appropriate and rapid method to screen potential inhibitors of hIAPP11‐20 or other amyloid proteins. This study also broadens the types of inhibitors on inhibiting amyloid formation of hIAPP.
A shows relative fluorescence of hIAPP11‐20 incubated with different lipopeptides and B shows the affinity between FITC‐labeled hIAPP11‐20 and C14R4 (the most potential inhibitor) by microscale thermophoresis. (Source: Journal of Peptide Science)
Source: Journal of Peptide Science - November 1, 2017 Category: Biochemistry Authors: Yexuan Mao, Lanlan Yu, Mengfan Mao, Chuanguo Ma, Lingbo Qu Tags: RESEARCH ARTICLE Source Type: research
Modulation of immunogenicity of poly(sarcosine) displayed on various nanoparticle surfaces due to different physical properties
Poly(sarcosine) displayed on polymeric micelle is reported to trigger a T cell‐independent type2 reaction with B1a cells in the mice to produce IgM and IgG3 antibodies. In addition to polymeric micelle, three kinds of vesicles displaying poly(sarcosine) on surface were prepared here to evaluate the amounts and avidities of IgM and IgG3, which were produced in mice, to correlate them with physical properties of the molecular assemblies. The largest amount of IgM was produced after twice administrations of a polymeric micelle of 35 nm diameter (G1). On the other hand, the production amount of IgG3 became the largest after...
Source: Journal of Peptide Science - November 1, 2017 Category: Biochemistry Authors: Cheol Joo Kim, Eri Hara, Naoki Watabe, Isao Hara, Shunsaku Kimura Tags: Research Article Source Type: research
A comparative study of synthetic and semisynthetic approaches for ligating the epidermal growth factor to a bivalent scaffold
We report the successful generation of synthetic and semisynthetic ISEr‐EGF2 as well as several challenges encountered during the synthesis and ligations. We demonstrate the application of native chemical ligation for the design of larger ISEr constructs, facilitating new objectives for the coupling of small binder peptides and larger proteins to multivalent ISEr scaffolds. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
Native chemical ligation was used to ligate two molecules of the epidermal growth factor (EGF) to a bivalent polyethylene glycol scaffold. The advantages and disadvantages of ...
Source: Journal of Peptide Science - November 1, 2017 Category: Biochemistry Authors: Anna Lena Gell, Nadja Groysbeck, Christian F. W. Becker, Anne C. Conibear Tags: Research Article Source Type: research
GUB06 ‐046, a novel secretin/glucagon‐like peptide 1 co‐agonist, decreases food intake, improves glycemic control, and preserves beta cell mass in diabetic mice
Bariatric surgery is currently the most effective treatment of obesity, which has spurred an interest in developing pharmaceutical mimetics. It is thought that the marked body weight‐lowering effects of bariatric surgery involve stimulated secretion of appetite‐regulating gut hormones, including glucagon‐like peptide 1. We here report that intestinal expression of secretin is markedly upregulated in a rat model of Roux‐en‐Y gastric bypass, suggesting an additional role of secretin in the beneficial metabolic effects of Roux‐en‐Y gastric bypass. We therefore developed novel secretin‐based peptide co‐agonis...
Source: Journal of Peptide Science - October 23, 2017 Category: Biochemistry Authors: S øren B. Witteloostuijn, Louise S. Dalbøge, Gitte Hansen, Søren Roi Midtgaard, Grethe Vestergaard Jensen, Knud J. Jensen, Niels Vrang, Jacob Jelsing, Søren L. Pedersen Tags: Research Article Source Type: research
Issue information
(Source: Journal of Peptide Science)
Source: Journal of Peptide Science - October 16, 2017 Category: Biochemistry Tags: Issue Information Source Type: research
The American cockroach peptide periplanetasin ‐4 inhibits Clostridium difficile toxin A‐induced cell toxicities and inflammatory responses in the mouse gut
Many reports have shown that crude extracts of the American cockroach have therapeutic effects on inflammation. In a previous study, our research group showed that an antimicrobial peptide (Periplanetasin‐2) derived from the American cockroach via de novo transcriptome analysis inhibited apoptosis of human colonocytes and inflammatory responses of the mouse gut caused by Clostridium difficile toxin A. Here, we examined whether Periplanetasin‐4 (Peri‐4), another antimicrobial peptide identified via de novo transcriptome analysis of the American cockroach, could also inhibit the various toxicities induced by C. diffic...
Source: Journal of Peptide Science - September 26, 2017 Category: Biochemistry Authors: I Na Yoon, Li Fang Lu, Ji Hong, Peng Zhang, Dae Hong Kim, Jin Ku Kang, Jae Sam Hwang, Ho Kim Tags: Research Article Source Type: research
Mikl ós Bodanszky Award Lecture: Advances in the selective targeting of protein phosphatase‐1 and phosphatase‐2A with peptides
Protein phosphatase‐1 and phosphatase‐2A are two ubiquitously expressed enzymes known to catalyze the majority of dephosphorylation reactions on serine and threonine inside cells. They play roles in most cellular processes and are tightly regulated by regulatory subunits in holoenzymes. Their misregulation and malfunction contribute to disease development and progression, such as in cancer, diabetes, viral infections, and neurological as well as heart diseases. Therefore, targeting these phosphatases for therapeutic use would be highly desirable; however, their complex regulation and high conservation of the active sit...
Source: Journal of Peptide Science - September 5, 2017 Category: Biochemistry Authors: Maja K öhn Tags: Review Source Type: research
Issue information
(Source: Journal of Peptide Science)
Source: Journal of Peptide Science - September 5, 2017 Category: Biochemistry Tags: Issue Information Source Type: research
Intramolecular cyclization of the antimicrobial peptide Polybia ‐MPI with triazole stapling: influence on stability and bioactivity
In conclusion, the design and synthesis of cyclic analogs via click chemistry offer a new strategy for the development of stable antimicrobial agents. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
The MPI cyclic analogs C‐MPI‐1 (i‐ to ‐i+4) and C‐MPI‐2 (i‐ to ‐i+6) were synthesized by copper(I)‐catalyzed azide‐alkyne cycloaddition. Cyclic analogs have different bioactivity, and especially, C‐MPI‐1 has enhanced anti‐trypsin resistance compared with the parent peptide MPI. The mechanism of action and the secondary structure of all peptides were further investigated. (So...
Source: Journal of Peptide Science - August 23, 2017 Category: Biochemistry Authors: Beijun Liu, Wei Zhang, Sanhu Gou, Haifeng Huang, Jia Yao, Zhibin Yang, Hui Liu, Chao Zhong, Beiyin Liu, Jingman Ni, Rui Wang Tags: Research Article Source Type: research
Self ‐assembled chiral nanostructures of amphiphilic peptide: from single molecule to aggregate
We report interesting hierarchical self‐assembled architectures from a designed amphiphilic peptide. The bisignate cotton effects in circular dichroism spectra show typical peptide aggregation‐induced. The observation of peptide assembly structures from initial particles and fibrils to ribbon structures is supported by microscopy (atomic force microscopy and transmission electron microscopy). The visualization of individual peptide at the single molecular level offered insights of the intermolecular interactions responsible for the formation of aggregates, which is investigated by scanning tunneling microscopy. The ori...
Source: Journal of Peptide Science - August 16, 2017 Category: Biochemistry Authors: Ting Zhou, Zhiqing Zhang, Xuemei Zhang, Chen Wang, Guiying Xu, Yanlian Yang Tags: Rapid Communication Source Type: research
