A comparative study of synthetic and semisynthetic approaches for ligating the epidermal growth factor to a bivalent scaffold

We report the successful generation of synthetic and semisynthetic ISEr‐EGF2 as well as several challenges encountered during the synthesis and ligations. We demonstrate the application of native chemical ligation for the design of larger ISEr constructs, facilitating new objectives for the coupling of small binder peptides and larger proteins to multivalent ISEr scaffolds. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. Native chemical ligation was used to ligate two molecules of the epidermal growth factor (EGF) to a bivalent polyethylene glycol scaffold. The advantages and disadvantages of synthetic and semisynthetic approaches to generating the EGF thioesters are discussed.
Source: Journal of Peptide Science - Category: Biochemistry Authors: Tags: Research Article Source Type: research