High ‐molecular‐weight poly(Gly‐Val‐Gly‐Val‐Pro) synthesis through microwave irradiation
In this study, we synthesized a polypeptide from its pentapeptide unit using microwave irradiation. Effective methods for polypeptide synthesis from unit peptides have not been reported. Here, we used a key elastin peptide, H‐GlyValGlyValPro‐OH (GVGVP), as the monomer peptide. It is difficult to obtain poly(Gly‐Val‐Gly‐Val‐Pro) (poly(GVGVP)) from the pentapeptide unit of elastin, GVGVP, via polycondensation. Poly(GVGVP) prepared from genetically recombinant Escherichia coli is a well‐known temperature‐sensitive polypeptide, and this temperature sensitivity is known as the lower critical solution temperature...
Source: Journal of Peptide Science - June 28, 2016 Category: Biochemistry Authors: Mitsuaki Goto, Takeshi Endo Tags: Research Article Source Type: research
Identification of a female spawn‐associated Kazal‐type inhibitor from the tropical abalone Haliotis asinina
Abalone (Haliotis) undergoes a period of reproductive maturation, followed by the synchronous release of gametes, called broadcast spawning. Field and laboratory studies have shown that the tropical species Haliotis asinina undergoes a two‐week spawning cycle, thus providing an excellent opportunity to investigate the presence of endogenous spawning‐associated peptides. In female H. asinina, we have isolated a peptide (5145 Da) whose relative abundance in hemolymph increases substantially just prior to spawning and is still detected using reverse‐phase high‐performance liquid chromatography chromatograms up to 1...
Source: Journal of Peptide Science - June 28, 2016 Category: Biochemistry Authors: Tianfang Wang, Parinyaporn Nuurai, Carmel McDougall, Patrick S. York, Utpal Bose, Bernard M. Degnan, Scott F. Cummins Tags: Research Article Source Type: research
High‐molecular‐weight poly(Gly‐Val‐Gly‐Val‐Pro) synthesis through microwave irradiation
In this study, we synthesized a polypeptide from its pentapeptide unit using microwave irradiation. Effective methods for polypeptide synthesis from unit peptides have not been reported. Here, we used a key elastin peptide, H‐GlyValGlyValPro‐OH (GVGVP), as the monomer peptide. It is difficult to obtain poly(Gly‐Val‐Gly‐Val‐Pro) (poly(GVGVP)) from the pentapeptide unit of elastin, GVGVP, via polycondensation. Poly(GVGVP) prepared from genetically recombinant Escherichia coli is a well‐known temperature‐sensitive polypeptide, and this temperature sensitivity is known as the lower critical solution temperature...
Source: Journal of Peptide Science - June 28, 2016 Category: Biochemistry Authors: Mitsuaki Goto, Takeshi Endo Tags: Research Article Source Type: research
Short AntiMicrobial Peptides (SAMPs) as a class of extraordinary promising therapeutic agents
The emergence of multidrug resistant bacteria has a direct impact on global public health because of the reduced potency of existing antibiotics against pathogens. Hence, there is a pressing need for new drugs with different modes of action that can kill microorganisms. Antimicrobial peptides (AMPs) can be regarded as an alternative tool for this purpose because they are proven to have therapeutic effects with broad‐spectrum activities. There are some hurdles in using AMPs as clinical candidates such as toxicity, lack of stability and high budgets required for manufacturing. This can be overcome by developing shorter and...
Source: Journal of Peptide Science - June 28, 2016 Category: Biochemistry Authors: Suhas Ramesh, Thavendran Govender, Hendrik G. Kruger, Beatriz G. Torre, Fernando Albericio Tags: Review Source Type: research
Site‐specific conjugation of the quencher on peptide's N‐terminal for the synthesis of a targeted non‐spreading activatable optical probe
Optical imaging offers high sensitivity and portability at low cost. The design of ‘smart’ or ‘activatable’ probes can decrease the background noise and increase the specificity of the signal. By conjugating a fluorescent dye and a compatible quencher on each side of an enzyme's substrate, the signal remains in its ‘off ’ state until it reaches the area where a specific enzyme is expressed. However, the signal can leak from that area unless the dye is attached to a molecule able to bind to a specific target also presented in that area. The aim of this study was to (i) specifically conjugate the quencher on the ...
Source: Journal of Peptide Science - June 9, 2016 Category: Biochemistry Authors: Bryan Simard, Gleb G. Mironov, Boguslaw Tomanek, Frank C. J. M. Veggel, Abedelnasser Abulrob Tags: Research Article Source Type: research
Beta amino acid‐modified and fluorescently labelled kisspeptin analogues with potent KISS1R activity
Kisspeptin analogues with improved metabolic stability may represent important ligands in the study of the kisspeptin/KISS1R system and have therapeutic potential. In this paper we assess the activity of known and novel kisspeptin analogues utilising a dual luciferase reporter assay in KISS1R‐transfected HEK293T cells. In general terms the results reflect the outcomes of other assay formats and a number of potent agonists were identified among the analogues, including β2‐hTyr‐modified and fluorescently labelled forms. We also showed, by assaying kisspeptin in the presence of protease inhibitors, that proteolysis of ...
Source: Journal of Peptide Science - June 9, 2016 Category: Biochemistry Authors: M. A. Camerino, M. Liu, S. Moriya, T. Kitahashi, A. Mahgoub, S. J. Mountford, D. K. Chalmers, T. Soga, I. S. Parhar, P. E. Thompson Tags: Research Article Source Type: research
Comparison of marmoset and human FSH using synthetic peptides of the β‐subunit L2 loop region and anti‐peptide antibodies
In this study we attempted to understand some of these structural differences using FSH peptides and anti‐peptide antibody approach. Based on sequence alignment, in silico modeling and docking studies, L2 loop of FSH β‐subunit (L2β) was found to be different between marmoset and human. Hence, peptides corresponding to region 32–50 of marmoset and human L2β loop were synthesized, purified and characterized. The peptides displayed dissimilarity in terms of molecular mass, predicted isoelectric point, predicted charge and in the ability to inhibit hormone–receptor interaction. Polyclonal antibodies generated agains...
Source: Journal of Peptide Science - June 9, 2016 Category: Biochemistry Authors: Susha S. Kutteyil, Bhalchandra J. Kulkarni, Rahul Mojidra, Shaini Joseph, Bhakti R. Pathak, Smita D. Mahale Tags: Research Article Source Type: research
Peptide nanotube aligning side chains onto one side
A novel pseudo cyclic penta‐β‐peptide composed of a β‐naphthylalanine, two β‐alanines, and a sequence of ethylenediamine‐succinic acid (CP5ES) is synthesized and investigated on peptide nanotube (PNT) formation. When the PNT is formed with the maximum number of intermolecular hydrogen bonds between the cyclic peptides, the sequence enables the alignment of the side chains, naphthyl groups, on one side of the PNT. Microscopic and spectroscopic observations of CP5ES crystals reveal that CP5ES forms rod‐ or needle‐shaped molecular assemblies showing exciton coupling of the Cotton effect and predominant monome...
Source: Journal of Peptide Science - June 9, 2016 Category: Biochemistry Authors: Yuki Tabata, Shota Mitani, Shunsaku Kimura Tags: Research Article Source Type: research
Peptide purification using the chemoselective reaction between N‐(methoxy)glycine and isothiocyanato‐functionalized resin
An efficient peptide purification strategy is established, comprising the selective reaction of an N‐terminal N‐(methoxy)glycine residue of the peptide and isothiocyanato‐functionalized resins, and subsequent Edman degradation. These reactions take place in acidic media; in particular, the Edman degradation proceeds smoothly in media containing more than 50% trifluoroacetic acid (v/v). These acidic conditions offer increased solubility, making them advantageous for the purification of hydrophobic and aggregation‐prone peptides. The effectiveness of this method, together with scope and limitations, is demonstrated u...
Source: Journal of Peptide Science - June 9, 2016 Category: Biochemistry Authors: Toshiaki Hara, Akira Tainosyo, Toru Kawakami, Saburo Aimoto, Michio Murata Tags: Rapid Communication Source Type: research
Issue Information
(Source: Journal of Peptide Science)
Source: Journal of Peptide Science - June 9, 2016 Category: Biochemistry Tags: Issue Information Source Type: research
Anti ‐biofilm and sporicidal activity of peptides based on wheat puroindoline and barley hordoindoline proteins
This study shows that tryptophan‐rich antimicrobial peptides (AMPs), designed based on wheat puroindoline proteins, are highly effective against biofilms of Pseudomonas aeruginosa and Listeria monocytogenes and endospores of Bacillus subtilis. The results establish their potential as novel biocidal agents. (Source: Journal of Peptide Science)
Source: Journal of Peptide Science - May 29, 2016 Category: Biochemistry Authors: Nadin Shagaghi, Rebecca L. Alfred, Andrew H. A. Clayton, Enzo A. Palombo, Mrinal Bhave Tags: Research Article Source Type: research
A dimer model of human calcitonin13 ‐32 forms an α‐helical structure and robustly aggregates in 50% aqueous 2,2,2‐trifluoroethanol solution
We report that a dimer model of human calcitonin13‐32 (hCT13‐32) aggregated to a greater degree than native hCT under aqueous TFE conditions. Analyses using circular dichroism spectroscopy, thioflavine‐T binding assays and atomic force microscopy suggest that the α‐helical portion of hCT is important for initiation of the aggregation process, which yields long fibrils. Our findings could potentially serve as the basis for development of novel hCT derivatives that could be utilized for treatment of hypercalcemia. (Source: Journal of Peptide Science)
Source: Journal of Peptide Science - May 29, 2016 Category: Biochemistry Authors: Hiroyuki Kawashima, Mei Katayama, Ryota Yoshida, Kenichi Akaji, Akiko Asano, Mitsunobu Doi Tags: Research Article Source Type: research
A dimer model of human calcitonin13‐32 forms an α‐helical structure and robustly aggregates in 50% aqueous 2,2,2‐trifluoroethanol solution
We report that a dimer model of human calcitonin13‐32 (hCT13‐32) aggregated to a greater degree than native hCT under aqueous TFE conditions. Analyses using circular dichroism spectroscopy, thioflavine‐T binding assays and atomic force microscopy suggest that the α‐helical portion of hCT is important for initiation of the aggregation process, which yields long fibrils. Our findings could potentially serve as the basis for development of novel hCT derivatives that could be utilized for treatment of hypercalcemia. (Source: Journal of Peptide Science)
Source: Journal of Peptide Science - May 29, 2016 Category: Biochemistry Authors: Hiroyuki Kawashima, Mei Katayama, Ryota Yoshida, Kenichi Akaji, Akiko Asano, Mitsunobu Doi Tags: Research Article Source Type: research
Innovative chemical synthesis and conformational hints on the lipopeptide liraglutide
Liraglutide is a new generation lipopeptide drug used for the treatment of type II diabetes. In this work, we describe new approaches for its preparation fully by chemical methods. The key step of these strategies is the synthesis in solution of the Lys/γ‐Glu building block, Fmoc‐Lys‐(Pal‐γ‐Glu‐OtBu)‐OH, in which Lys and Glu residues are linked through their side chains and γ‐Glu is Nα‐palmitoylated. This dipeptide derivative is then inserted into the peptide sequence on solid phase. As liraglutide is obtained with great purity and high yield, our approach can be particularly attractive for an industr...
Source: Journal of Peptide Science - May 29, 2016 Category: Biochemistry Authors: Ivan Guryanov, Alex Bondesan, Dario Visentini, Andrea Orlandin, Barbara Biondi, Claudio Toniolo, Fernando Formaggio, Antonio Ricci, Jacopo Zanon, Walter Cabri Tags: Research Article Source Type: research
Membrane damage as first and DNA as the secondary target for anti‐candidal activity of antimicrobial peptide P7 derived from cell‐penetrating peptide ppTG20 against Candida albicans
In this study, we investigated the antifungal effect of P7 and its anti‐Candida acting mode in Candida albicans. P7 displayed antifungal activity against the reference C. albicans (MIC = 4 μM), Aspergilla niger (MIC = 32 μM), Aspergillus flavus (MIC = 8 μM), and Trichopyton rubrum (MIC = 16 μM). The effect of P7 on the C. albicans cell membrane was examined by investigating the calcein leakage from fungal membrane models made of egg yolk l‐phosphatidylcholine/ergosterol (10 : 1, w/w) liposomes. P7 showed potent leakage effects against fungal liposomes similar to Melittin‐treated cells....
Source: Journal of Peptide Science - May 19, 2016 Category: Biochemistry Authors: Lirong Li, Fengxia Song, Jin Sun, Xu Tian, Shufang Xia, Guowei Le Tags: Research Article Source Type: research
