Potential of CLSM in studying some modern and fossil palynological objects
Summary
We have tested possibilities and limitations of confocal laser scanning microscopy to study the morphology of pollen and spores and inner structure of sporoderms. As test objects, we used pollen grains of the modern angiosperm Ribes niveum (Grossulariaceae) and Datura metel (Solanaceae), fossil angiosperm pollen grains of Pseudointegricorpus clarireticulatum and Wodehouseia spinata dated to the Late Cretaceous, fossil gymnosperm pollen grains of Cycadopites‐type dated to the Middle Jurassic, and fossil megaspores Maexisporites rugulaeferus, M. grosstriletus, and Trileites sp. dated to the Early Triassic. For comp...
Source: Journal of Microscopy - September 21, 2017 Category: Laboratory Medicine Authors: O. GAVRILOVA, N. ZAVIALOVA, M. TEKLEVA, E. KARASEV Tags: Original Article Source Type: research
Effects of different preprocessing algorithms on the prognostic value of breast tumour microscopic images
Journal of Microscopy,Volume 270, Issue 1, Page 17-26, April 2018. (Source: Journal of Microscopy)
Source: Journal of Microscopy - September 21, 2017 Category: Laboratory Medicine Source Type: research
---
Journal of Microscopy,Volume 270, Issue 1, Page 17-26, April 2018. (Source: Journal of Microscopy)
Source: Journal of Microscopy - September 21, 2017 Category: Laboratory Medicine Source Type: research
Effects of different preprocessing algorithms on the prognostic value of breast tumour microscopic images
Summary
The purpose of this study was to improve the prognostic value of tumour histopathology image analysis methodology by image preprocessing.
Key image qualities were modified including contrast, sharpness and brightness. The texture information was subsequently extracted from images of haematoxylin/eosin‐stained tumour tissue sections by GLCM, monofractal and multifractal algorithms without any analytical limitation to predefined structures. Images were derived from patient groups with invasive breast carcinoma (BC, 93 patients) and inflammatory breast carcinoma (IBC, 51 patients).
The prognostic performance was ind...
Source: Journal of Microscopy - September 21, 2017 Category: Laboratory Medicine Authors: D. KOLAREVI Ć, T. VUJASINOVIĆ, K. KANJER, J. MILOVANOVIĆ, N. TODOROVIĆ‐RAKOVIĆ, D. NIKOLIĆ‐VUKOSAVLJEVIĆ, M. RADULOVIC Tags: Original Article Source Type: research
Accurate representation of interference colours (Michel –Lévy chart): from rendering to image colour correction
This study presents a work flow towards an accurate representation of interference colours captured on a light microscope. The work flow includes accurate calculation of interference colours considering the colour tint of the light source of the microscope, as well as the colour correction of the camera using readily available colour targets.
The overall performance of the workflow was tested by comparing calculated interference colours with colour‐corrected images of a quartz wedge captured at 5× magnification. The performance was measured in units of ΔE00, with lower units signalling less colour difference and units ...
Source: Journal of Microscopy - September 21, 2017 Category: Laboratory Medicine Authors: S. A. LINGE JOHNSEN, J. BOLLMANN, H. W. LEE, Y. ZHOU Tags: Original Article Source Type: research
TOC ‐ Issue Information
(Source: Journal of Microscopy)
Source: Journal of Microscopy - September 19, 2017 Category: Laboratory Medicine Tags: Issue Information Source Type: research
A three ‐dimensional statistical model for imaged microstructures of porous polymer films
Summary
A thresholded Gaussian random field model is developed for the microstructure of porous materials. Defining the random field as a solution to stochastic partial differential equation allows for flexible modelling of nonstationarities in the material and facilitates computationally efficient methods for simulation and model fitting. A Markov Chain Monte Carlo algorithm is developed and used to fit the model to three‐dimensional confocal laser scanning microscopy images. The methods are applied to study a porous ethylcellulose/hydroxypropylcellulose polymer blend that is used as a coating to control drug release fr...
Source: Journal of Microscopy - September 1, 2017 Category: Laboratory Medicine Authors: S. BARMAN, D. BOLIN Tags: Original Article Source Type: research
Reconstructing the microstructure of polyimide –silicalite mixed‐matrix membranes and their particle connectivity using FIB‐SEM tomography
Summary
Properties of a composite material made of a continuous matrix and particles often depend on microscopic details, such as contacts between particles. Focusing on processing raw focused‐ion beam scanning electron microscope (FIB‐SEM) tomography data, we reconstructed three mixed‐matrix membrane samples made of 6FDA‐ODA polyimide and silicalite‐1 particles. In the first step of image processing, backscattered electron (BSE) and secondary electron (SE) signals were mixed in a ratio that was expected to obtain a segmented 3D image with a realistic volume fraction of silicalite‐1. Second, after spatial align...
Source: Journal of Microscopy - September 1, 2017 Category: Laboratory Medicine Authors: P. DIBL ÍKOVÁ, M. VESELÝ, P. SYSEL, P. ČAPEK Tags: Original Article Source Type: research
Geometrical characterization of fluorescently labelled surfaces from noisy 3D microscopy data
We present a fully automated algorithm to determine the location and curvatures of an object from 3D fluorescence images, such as those obtained using confocal or light‐sheet microscopy. The algorithm aims at reconstructing surface labelled objects with spherical topology and mild deformations from the spherical geometry with high accuracy, rather than reconstructing arbitrarily deformed objects with lower fidelity. Using both synthetic data with known geometrical characteristics and experimental data of spherical objects, we characterize the algorithm's accuracy over the range of conditions and parameters typically enco...
Source: Journal of Microscopy - September 1, 2017 Category: Laboratory Medicine Authors: ELIJAH SHELTON, FRIEDHELM SERWANE, OTGER CAMP ÀS Tags: Original Article Source Type: research
Single particle raster image analysis of diffusion for particle mixtures
Summary
Recently we complemented the raster image correlation spectroscopy (RICS) method of analysing raster images via estimation of the image correlation function with the method single particle raster image analysis (SPRIA). In SPRIA, individual particles are identified and the diffusion coefficient of each particle is estimated by a maximum likelihood method. In this paper, we extend the SPRIA method to analyse mixtures of particles with a finite set of diffusion coefficients in a homogeneous medium. In examples with simulated and experimental data with two and three different diffusion coefficients, we show that SPRIA...
Source: Journal of Microscopy - September 1, 2017 Category: Laboratory Medicine Authors: M. LONGFILS, M. R ÖDING, A. ALTSKÄR, E. SCHUSTER, N. LORÉN, A. SÄRKKÄ, M. RUDEMO Tags: Original Article Source Type: research
Surface relaxation of strained Ga(P,As)/GaP heterostructures investigated by HAADF stem
Summary
The surfaces of thin transmission electron microscopy (TEM) specimens of strained heterostructures can relax. The resulting bending of the lattice planes significantly influences high‐angle annular dark field (HAADF) measurements. We investigate the impact by evaluating the intensities measured at the atomic columns as well as their positions in high‐resolution HAADF images. In addition, the consequences in the diffraction plane will be addressed by simulated position averaged convergent beam electron diffraction (PACBED) patterns.
The experimental column intensities and positions acquired from a strained Ga(P,...
Source: Journal of Microscopy - September 1, 2017 Category: Laboratory Medicine Authors: ANDREAS BEYER, LENNART DUSCHEK, J ÜRGEN BELZ, JAN OLIVER OELERICH, KAKHABER JANDIERI, KERSTIN VOLZ Tags: Themed Issue Paper Source Type: research
In situ oxidation and reduction of triangular nickel nanoplates via environmental transmission electron microscopy
Summary
Understanding the oxidation and reduction mechanisms of transition metals, such as nickel (Ni), is important for their use in industrial applications of catalysis. A powerful technique for investigating the redox reactive species is in situ environmental transmission electron microscopy (ETEM), where oxidation and reduction can be tracked in real time. One particular difficulty in understanding the underlying reactions is understanding the underlying morphology of the starting structure in a reaction, in particular the defects contained in the material, and the exposed surface facets. Here‐in, we use a colloidal ...
Source: Journal of Microscopy - August 29, 2017 Category: Laboratory Medicine Authors: A.P. LAGROW, N.M. ALYAMI, D.C. LLOYD, O.M. BAKR, E.D. BOYES, P.L. GAI Tags: Themed Issue Paper Source Type: research
A 340/380 nm light ‐emitting diode illuminator for Fura‐2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision
In this report, we demonstrate the first application of a 340/380 nm LED illuminator for ratiometric Fura‐2 calcium imaging of live cells. This illuminator uses wavelengths more accurately matching the peak wavelengths of Fura‐2, and we found that this conferred several advantages over existing illuminators. We found that it is possible to detect changes in intracellular calcium with a precision of better than 5 nM. Because Fura‐2 has a theoretical precision of 5–10 nM, our instrument performance was only limited by the response of the dye. We also explored the prospect of using lower concentrations of Fura‐2 tha...
Source: Journal of Microscopy - August 24, 2017 Category: Laboratory Medicine Authors: P. W. TINNING, A. J. P.M. FRANSSEN, S. U. HRIDI, T. J. BUSHELL, G. MCCONNELL Tags: Original Article Source Type: research
Analysis of liquid suspensions using scanning electron microscopy in transmission: estimation of the water film thickness using Monte –Carlo simulations
In this study, we characterise suspensions of gold and core‐shell polymer particles in water with a dedicated scanning electron microscope. As the liquid state is ensured by controlling the liquid temperature and the pressure of vapour around the sample, it is in practice very difficult to control the thickness of the water droplet. We use here numerical simulations to better understand the contrast between the core, the shell and water, and to give an estimation of the water droplet thickness. (Source: Journal of Microscopy)
Source: Journal of Microscopy - August 22, 2017 Category: Laboratory Medicine Authors: J. XIAO, G. FORAY, K. MASENELLI ‐VARLOT Tags: Themed Issue Paper Source Type: research
Phase imaging quality improvement by modification of AFM probes ’ cantilever
In this study, the circularly shaped holes located in varying distance from the probe base, were cut out by focused ion beam. Such modification was a consequence of the results of the previous experiments (probe tip sharpening and cantilever thinning) where significant improvement of image quality in tapping and phase imaging mode has been revealed. The solution proposed herein gives similar results, but is much simpler from the technological point of view. Shorter exposition time of the tip onto gallium ions during FIB processing allows to reduce material degradation. The aim of this modification was to change harmonic os...
Source: Journal of Microscopy - August 16, 2017 Category: Laboratory Medicine Authors: J. SKIBINSKI, J. REBIS, L. KACZMAREK, T. WEJRZANOWSKI, T. PLOCINSKI, K. ROZNIATOWSKI Tags: Original Article Source Type: research
