Oolong tea and LR ‐White resin: a new method of plant sample preparation for transmission electron microscopy
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 15, 2018 Category: Laboratory Medicine Source Type: research
A guide to analysis and reconstruction of serial block face scanning electron microscopy data
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 15, 2018 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 15, 2018 Category: Laboratory Medicine Source Type: research
Measurement of two ‐photon‐absorption spectra through nonlinear fluorescence produced by a line‐shaped excitation beam
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 11, 2018 Category: Laboratory Medicine Source Type: research
Analogous on ‐axis interference topographic phase microscopy (AOITPM)
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 11, 2018 Category: Laboratory Medicine Source Type: research
On the influence of lipid ‐induced optical anisotropy for the bioimaging of exo‐ or endocytosis with interference microscopic imaging
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 11, 2018 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 11, 2018 Category: Laboratory Medicine Source Type: research
Measurement of two ‐photon‐absorption spectra through nonlinear fluorescence produced by a line‐shaped excitation beam
We present a newly conceived experimental technique to measure the two‐photon‐absorption spectra of fluorescent materials. The proposed method is based on the two‐photon fluorescence, but it permits to overcome the typical drawback of such a technique, consisting of the very low signal level, which usually is maintained in a regime of photon counting. In our proposed method the optical excitation of the nonlinear fluorescence is produced by employing a line‐shaped laser beam. This configuration, which permits to maintain the intrinsic high selectivity on two‐photon‐absorption signal which is typical of fluoresc...
Source: Journal of Microscopy - January 11, 2018 Category: Laboratory Medicine Authors: E. HASANI, J. PARRAVICINI, L. TARTARA, A. TOMASELLI, D. TOMASSINI Tags: Original Article Source Type: research
An optical configuration for fastidious STEM detector calibration and the effect of the objective ‐lens pre‐field
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 8, 2018 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 8, 2018 Category: Laboratory Medicine Source Type: research
A multimodal microcharacterisation of trace ‐element zonation and crystallographic orientation in natural cassiterite by combining cathodoluminescence, EBSD, EPMA and contribution of confocal Raman‐in‐SEM imaging
We present a multitechnical approach to chemical and structural data that includes scanning electron microscopy (SEM)‐based imaging and microanalysis techniques such as: secondary and backscattered electrons, cathodoluminescence (CL), electron probe microanalyser (EPMA), electron backscattered diffraction (EBSD) and confocal Raman‐imaging integrated in a SEM (RISE). The presented results show the complementarity of the used analytical techniques. SEM, CL, EBSD, EPMA provide information from the interaction of an electron beam with minerals, leading to atomistic information about their composition, whereas RISE, Raman s...
Source: Journal of Microscopy - January 1, 2018 Category: Laboratory Medicine Authors: G. WILLE, C. LEROUGE, U. SCHMIDT Tags: Original Article Source Type: research
Oolong tea and LR ‐White resin: a new method of plant sample preparation for transmission electron microscopy
Summary
Simplifying sample processing, shortening the sample preparation time, and adjusting procedures to suitable for new health and safety regulations, these issues are the current challenges which electron microscopic examinations need to face. In order to resolve these problems, new plant tissue sample processing protocols for transmission electron microscopy should be developed. In the present study, we chose the LR‐White resin‐assisted processing protocol for the ultrastructural observation of different types of plant tissues. Moreover, we explored Oolong tea extract (OTE) as a substitute for UA in staining ultr...
Source: Journal of Microscopy - January 1, 2018 Category: Laboratory Medicine Authors: XIAOHUA HE, FUZHEN GUO, BIN LIU Tags: Original Article Source Type: research
