Spin ‐coated epoxy resin embedding technique enables facile SEM/FIB thickness determination of porous metal oxide ultra‐thin films
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 25, 2018 Category: Laboratory Medicine Source Type: research
Spectroscopic identification of individual fluorophores using photoluminescence excitation spectra
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 25, 2018 Category: Laboratory Medicine Source Type: research
---
Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 25, 2018 Category: Laboratory Medicine Source Type: research
Spectroscopic identification of individual fluorophores using photoluminescence excitation spectra
We report a high throughput method for measuring the photoluminescence excitation spectra of individual fluorophores using a tunable supercontinuum laser and prism‐type total internal reflection fluorescence microscope. We used the system to measure and sort the photoluminescence excitation spectra of individual Alexa dyes, fluorescent nanodiamonds (FNDs), and fluorescent polystyrene beads. We used a Gaussian mixture model with maximum likelihood estimation to objectively separate the spectra. Finally, we spectroscopically identified different species of fluorescent nanodiamonds with overlapping spectra and characterized...
Source: Journal of Microscopy - January 25, 2018 Category: Laboratory Medicine Authors: J. CZERSKI, W. COLOMB, F. CANNATARO, S. K. SARKAR Tags: Original Article Source Type: research
An orientation ‐independent DIC microscope allows high resolution imaging of epithelial cell migration and wound healing in a cnidarian model
Summary
Epithelial cell dynamics can be difficult to study in intact animals or tissues. Here we use the medusa form of the hydrozoan Clytia hemisphaerica, which is covered with a monolayer of epithelial cells, to test the efficacy of an orientation‐independent differential interference contrast microscope for in vivo imaging of wound healing. Orientation‐independent differential interference contrast provides an unprecedented resolution phase image of epithelial cells closing a wound in a live, nontransgenic animal model. In particular, the orientation‐independent differential interference contrast microscope equipp...
Source: Journal of Microscopy - January 18, 2018 Category: Laboratory Medicine Authors: J.E. MALAMY, M. SHRIBAK Tags: Original Article Source Type: research
An orientation ‐independent DIC microscope allows high resolution imaging of epithelial cell migration and wound healing in a cnidarian model
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 18, 2018 Category: Laboratory Medicine Source Type: research
---
Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 18, 2018 Category: Laboratory Medicine Source Type: research
Environmental electron microscopy: materials in their real live in gas or liquid
(Source: Journal of Microscopy)
Source: Journal of Microscopy - January 18, 2018 Category: Laboratory Medicine Authors: THIERRY EPICIER, MARK RAINFORTH Tags: Introduction Source Type: research
TOC ‐ Issue Information
(Source: Journal of Microscopy)
Source: Journal of Microscopy - January 18, 2018 Category: Laboratory Medicine Tags: Issue Information Source Type: research
Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 16, 2018 Category: Laboratory Medicine Source Type: research
A multimodal microcharacterisation of trace ‐element zonation and crystallographic orientation in natural cassiterite by combining cathodoluminescence, EBSD, EPMA and contribution of confocal Raman‐in‐SEM imaging
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 16, 2018 Category: Laboratory Medicine Source Type: research
---
Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 16, 2018 Category: Laboratory Medicine Source Type: research
Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography
Summary
The aim of this work is to make progress towards the development of 3D reconstruction as a legitimate alternative to traditional 2D characterization of soot. Time constraints are the greatest opposition to its implementation, as currently reconstruction of a single soot particle takes around 5–6 h to complete. As such, the accuracy and detail gains are currently insufficient to challenge 2D characterization of a representative sample (e.g. 200 particles). This work is a consideration of the optimization of the steps included within the computational reconstruction and manual segmentation of soot particles. Our op...
Source: Journal of Microscopy - January 16, 2018 Category: Laboratory Medicine Authors: E. HAFFNER ‐STATON, A. LA ROCCA, M.W. FAY Tags: Original Article Source Type: research
