The structure of InAlGaN layers grown by metal organic vapour phase epitaxy: effects of threading dislocations and inversion domains from the GaN template
Summary Defects in quaternary InAlGaN barriers and their effects on crystalline quality and surface morphology have been studied. In addition to growth conditions, the quality of the GaN template may play an important role in the formation of defects in the barrier. Therefore, this work is focused on effects caused by threading dislocations (TDs) and inversion domains (IDs) originating from the underlying GaN. The effects are observed on the crystalline quality of the barrier and characteristic surface morphologies. Each type of TDs is shown to affect the surface morphology in a different way. Depending on the size of the ...
Source: Journal of Microscopy - October 1, 2017 Category: Laboratory Medicine Authors: H. BEN AMMAR, A. MINJ, M. PIERRE ‐CHAUVAT, P. GAMARRA, C. LACAM, M. MORALES, P. RUTERANA Tags: Themed Issue Paper Source Type: research

Comparison of cross ‐sectional transmission electron microscope studies of thin germanium epilayers grown on differently oriented silicon wafers
Summary We compare transmission electron microscopical analyses of the onset of islanding in the germanium‐on‐silicon (Ge/Si) system for three different Si substrate orientations: (001), (10) and (11)Si. The Ge was deposited by reduced pressure chemical vapour deposition and forms islands on the surface of all Si wafers; however, the morphology (aspect ratio) of the deposited islands is different for each type of wafer. Moreover, the mechanism for strain relaxation is different for each type of wafer owing to the different orientation of the (111) slip planes with the growth surface. Ge grown on (001)Si is initially ps...
Source: Journal of Microscopy - October 1, 2017 Category: Laboratory Medicine Authors: D.J. NORRIS, M. MYRONOV, D.R. LEADLEY, T. WALTHER Tags: Themed Issue Paper Source Type: research

Structural characterization of SnS crystals formed by chemical vapour deposition
Summary The crystal and defect structure of SnS crystals grown using chemical vapour deposition for application in electronic devices are investigated. The structural analysis shows the presence of two distinct crystal morphologies, that is thin flakes with lateral sizes up to 50 m and nanometer scale thickness, and much thicker but smaller crystallites. Both show similar Raman response associated with SnS. The structural analysis with transmission electron microscopy shows that the flakes are single crystals of α‐SnS with [010] normal to the substrate. Parallel with the surface of the flakes, lamellae with varying thic...
Source: Journal of Microscopy - September 28, 2017 Category: Laboratory Medicine Authors: A. NALIN MEHTA, H. ZHANG, A. DABRAL, O. RICHARD, P. FAVIA, H. BENDER, A. DELABIE, M. CAYMAX, M. HOUSSA, G. POURTOIS, W. VANDERVORST Tags: Themed Issue Paper Source Type: research

Imaging analysis of the interface between osteoblasts and microrough surfaces of laser ‐sintered titanium alloy constructs
Journal of Microscopy,Volume 270, Issue 1, Page 41-52, April 2018. (Source: Journal of Microscopy)
Source: Journal of Microscopy - September 28, 2017 Category: Laboratory Medicine Source Type: research

Quantitative imaging of Candida utilis and its organelles by soft X ‐ray Nano‐CT
Journal of Microscopy,Volume 270, Issue 1, Page 64-70, April 2018. (Source: Journal of Microscopy)
Source: Journal of Microscopy - September 28, 2017 Category: Laboratory Medicine Source Type: research

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Journal of Microscopy,Volume 270, Issue 1, Page 41-52, April 2018. (Source: Journal of Microscopy)
Source: Journal of Microscopy - September 28, 2017 Category: Laboratory Medicine Source Type: research

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Journal of Microscopy,Volume 270, Issue 1, Page 64-70, April 2018. (Source: Journal of Microscopy)
Source: Journal of Microscopy - September 28, 2017 Category: Laboratory Medicine Source Type: research

Effective absorption correction for energy dispersive X ‐ray mapping in a scanning transmission electron microscope: analysing the local indium distribution in rough samples of InGaN alloy layers
Summary We have applied our previous method of self‐consistent k*‐factors for absorption correction in energy‐dispersive X‐ray spectroscopy to quantify the indium content in X‐ray maps of thick compound InGaN layers. The method allows us to quantify the indium concentration without measuring the sample thickness, density or beam current, and works even if there is a drastic local thickness change due to sample roughness or preferential thinning. The method is shown to select, point‐by‐point in a two‐dimensional spectrum image or map, the k*‐factor from the local Ga K/L intensity ratio that is most appropr...
Source: Journal of Microscopy - September 28, 2017 Category: Laboratory Medicine Authors: X. WANG, M. ‐P. CHAUVAT, P. RUTERANA, T. WALTHER Tags: Themed Issue Paper Source Type: research

Imaging analysis of the interface between osteoblasts and microrough surfaces of laser ‐sintered titanium alloy constructs
This study introduces scalable methods for viewing the cell‐surface interface across multiple imaging modalities for a single cell. (Source: Journal of Microscopy)
Source: Journal of Microscopy - September 28, 2017 Category: Laboratory Medicine Authors: A. CHENG, H. CHEN, Z. SCHWARTZ, B.D. BOYAN Tags: Original Article Source Type: research

Advanced spinning disk ‐TIRF microscopy for faster imaging of the cell interior and the plasma membrane
Summary Understanding the cellular processes that occur between the cytosol and the plasma membrane is an important task for biological research. Till now, however, it was not possible to combine fast and high‐resolution imaging of both the isolated plasma membrane and the surrounding intracellular volume. Here, we demonstrate the combination of fast high‐resolution spinning disk (SD) and total internal reflection fluorescence (TIRF) microscopy for specific imaging of the plasma membrane. A customised SD‐TIRF microscope was used with specific design of the light paths that allowed, for the first time, live SD‐TIRF ...
Source: Journal of Microscopy - September 28, 2017 Category: Laboratory Medicine Authors: BERND ZOBIAK, ANTONIO VIRGILIO FAILLA Tags: Original Article Source Type: research

Quantitative imaging of Candida utilis and its organelles by soft X ‐ray Nano‐CT
Summary Soft X‐ray microscopy has excellent characteristics for imaging cells and subcellular structures. In this paper, the yeast strain, Candida utilis, was imaged by soft X‐ray microscopy and three‐dimensional volumes were reconstructed with the SART‐TV method. We performed segmentation on the reconstruction in three dimensions and identified several types of subcellular architecture within the specimen cells based on their linear absorption coefficient (LAC) values. Organelles can be identified by the correlation between the soft X‐ray LAC values and the subcellular architectures. Quantitative analyses of the...
Source: Journal of Microscopy - September 28, 2017 Category: Laboratory Medicine Authors: J. LIU, F. LI, L. CHEN, Y. GUAN, L. TIAN, Y. XIONG, G. LIU, Y. TIAN Tags: Original Article Source Type: research

Differentiation of human hair by colour and diameter using light microscopy, digital imaging and statistical analysis
Summary This research introduces and evaluates a novel method that offers the potential of providing objective criteria to forensic microscopical hair comparisons. The method combines hair diameter with numeric characterisations of red, green, and blue colour content as determined with the use of digital imaging at defined locations of the hair. Thirty hairs were collected from each of twenty participants, all with naturally coloured brown hair. The hairs were examined with an Olympus BX53® polarising light microscope and digital images were viewed with an Olympus DP72® camera under 400× magnification. Using Olympus cel...
Source: Journal of Microscopy - September 27, 2017 Category: Laboratory Medicine Authors: M. MILLS, J. BONETTI, T. BRETTELL, L. QUARINO Tags: Original Article Source Type: research

Differentiation of human hair by colour and diameter using light microscopy, digital imaging and statistical analysis
Journal of Microscopy,Volume 270, Issue 1, Page 27-40, April 2018. (Source: Journal of Microscopy)
Source: Journal of Microscopy - September 27, 2017 Category: Laboratory Medicine Source Type: research

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Journal of Microscopy,Volume 270, Issue 1, Page 27-40, April 2018. (Source: Journal of Microscopy)
Source: Journal of Microscopy - September 27, 2017 Category: Laboratory Medicine Source Type: research

Atomic structure of ‘W’‐type quantum well heterostructures investigated by aberration‐corrected STEM
Summary The atomic structure of (GaIn)As/Ga(AsSb)/(GaIn)As‐‘W’‐type quantum well heterostructures (‘W’‐QWHs) is investigated by scanning transmission electron microscopy (STEM). These structures were grown by metal organic vapour phase epitaxy and are built for type‐II laser systems in the infrared wavelength regime. For two samples grown at 525°C and 550°C, intensity profiles are extracted from the STEM images for each sublattice separately. These intensity profiles are compared to the one obtained from an image simulation of an ideal ‘W’‐QWH that is modelled in close agreement with the experiment....
Source: Journal of Microscopy - September 27, 2017 Category: Laboratory Medicine Authors: P. K ÜKELHAN, A. BEYER, C. FUCHS, M.J. WESELOH, S.W. KOCH, W. STOLZ, K. VOLZ Tags: Themed Issue Paper Source Type: research