Quantitative imaging of Candida utilis and its organelles by soft X ‐ray Nano‐CT

Summary Soft X‐ray microscopy has excellent characteristics for imaging cells and subcellular structures. In this paper, the yeast strain, Candida utilis, was imaged by soft X‐ray microscopy and three‐dimensional volumes were reconstructed with the SART‐TV method. We performed segmentation on the reconstruction in three dimensions and identified several types of subcellular architecture within the specimen cells based on their linear absorption coefficient (LAC) values. Organelles can be identified by the correlation between the soft X‐ray LAC values and the subcellular architectures. Quantitative analyses of the volume ratio of organelles to whole cell in different phases were also carried out according to the three‐dimensional datasets. With such excellent features, soft X‐ray imaging has a great influence in the field of biological cellular and subcellular research. Lay description To observe and understand subcellular structures of cells, cryo soft X‐ray imaging is a promising and effective method. For identification of organelles inside the cell, different auxiliary imaging modalities are usually required, such as fluorescence microscopy, which will increase work and introduce toxicity to biological sample. In this work, absorption properties of soft X‐rays was used to identify the organelles inside the cell, because the absorption of soft X‐ray is an inherent property of substances. Quantitative analyses of the identified organelles of different cel...
Source: Journal of Microscopy - Category: Laboratory Medicine Authors: Tags: Original Article Source Type: research