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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - February 27, 2018 Category: Laboratory Medicine Source Type: research
Evaluation of contrasting techniques for X ‐ray imaging of velvet worms (Onychophora)
Summary
Non‐invasive imaging techniques like X‐ray computed tomography have become very popular in zoology, as they allow for simultaneous imaging of the internal and external morphology of organisms. Nevertheless, the effect of different staining approaches required for this method on samples lacking mineralized tissues, such as soft‐bodied invertebrates, remains understudied. Herein, we used synchrotron radiation‐based X‐ray micro‐computed tomography to compare the effects of commonly used contrasting approaches on onychophorans – soft‐bodied invertebrates important for studying animal evolution. Represen...
Source: Journal of Microscopy - February 22, 2018 Category: Laboratory Medicine Authors: HENRY JAHN, IVO DE SENA OLIVEIRA, VLADIMIR GROSS, CHRISTINE MARTIN, ALEXANDER HIPP, GEORG MAYER, J ÖRG U. HAMMEL Tags: Original Article Source Type: research
Evaluation of contrasting techniques for X ‐ray imaging of velvet worms (Onychophora)
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - February 22, 2018 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - February 22, 2018 Category: Laboratory Medicine Source Type: research
Simultaneous measurement of quantum yield ratio and absorption ratio between acceptor and donor by linearly unmixing excitation –emission spectra
Summary
Quantum yield ratio (QA/QD) and absorption ratio (KA/KD) in all excitation wavelengths used between acceptor and donor are indispensable to quantitative fluorescence resonance energy transfer (FRET) measurement based on linearly unmixing excitation–emission spectra (ExEm‐spFRET). We here describe an approach to simultaneously measure QA/QD and KA/KD values by linearly unmixing the excitation–emission spectra of at least two different donor–acceptor tandem constructs with unknown FRET efficiency. To measure the QA/QD and KA/KD values of Venus (V) to Cerulean (C), we used a wide‐field fluorescence microscop...
Source: Journal of Microscopy - February 13, 2018 Category: Laboratory Medicine Authors: C. ZHANG, F. LIN, M. DU, W. QU, Z. MAI, J. QU, T. CHEN Tags: Original Article Source Type: research
Simultaneous measurement of quantum yield ratio and absorption ratio between acceptor and donor by linearly unmixing excitation –emission spectra
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - February 13, 2018 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - February 13, 2018 Category: Laboratory Medicine Source Type: research
TOC ‐ Issue Information
(Source: Journal of Microscopy)
Source: Journal of Microscopy - February 13, 2018 Category: Laboratory Medicine Tags: Issue Information Source Type: research
Alpha particle spectroscopy using FNTD and SIM super ‐resolution microscopy
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - February 2, 2018 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - February 2, 2018 Category: Laboratory Medicine Source Type: research
Alpha particle spectroscopy using FNTD and SIM super ‐resolution microscopy
Summary
Structured illumination microscopy (SIM) for the imaging of alpha particle tracks in fluorescent nuclear track detectors (FNTD) was evaluated and compared to confocal laser scanning microscopy (CLSM). FNTDs were irradiated with an external alpha source and imaged using both methodologies. SIM imaging resulted in improved resolution, without increase in scan time. Alpha particle energy estimation based on the track length, direction and intensity produced results in good agreement with the expected alpha particle energy distribution. A pronounced difference was seen in the spatial scattering of alpha particles in th...
Source: Journal of Microscopy - February 1, 2018 Category: Laboratory Medicine Authors: J.J.M. KOUWENBERG, G.J. KREMERS, J.A. SLOTMAN, H.T. WOLTERBEEK, A.B. HOUTSMULLER, A.G. DENKOVA, A.J.J. BOS Tags: Original Article Source Type: research
Reflectance difference microscopy for nanometre thickness microstructure measurements
Abstract
The discontinuity of medium at the boundary produces optically anisotropic response which makes reflectance difference microscopy (RDM) a potential method for nanometre‐thickness microstructure measurements. Here, we present the methodology of RDM for the edge measurement of ultrathin microstructure. The RD signal of microstructure's boundary is mathematically deduced according to boundary condition and polarization optics theory. A normal‐incidence RDM setup was built simply with one linear polarizer, one liquid crystal variable retarder and one 5 × objective. Then, the performance of the developed setup was...
Source: Journal of Microscopy - January 31, 2018 Category: Laboratory Medicine Authors: C. HU, S. HUO, W. SHEN, Y. LI, X. HU Tags: Original Article Source Type: research
Reflectance difference microscopy for nanometre thickness microstructure measurements
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 30, 2018 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - January 30, 2018 Category: Laboratory Medicine Source Type: research
Spin ‐coated epoxy resin embedding technique enables facile SEM/FIB thickness determination of porous metal oxide ultra‐thin films
In this study we demonstrate that by spin coating a thin layer of epoxy resin on top of the FeOx layer it is possible to create a thin homogenous layer that has an even thickness throughout the sample. The resin layer provides stability to the porous FeOx film, which would otherwise be damaged by the milling action of the focused ion beam, and provides a low‐density layer that can connect the sample to ground using a layer of Iridium metal thus facilitating the dissipation of the electric charge imposed by the electron beam on the sample. This layer was applied without affecting the underlying FeOx and can be rapidly app...
Source: Journal of Microscopy - January 25, 2018 Category: Laboratory Medicine Authors: B. PE ÑA, G.RH. OWEN, K.E. DETTELBACH, C.P. BERLINGUETTE Tags: Original Article Source Type: research
