Structure and composition of Unio pictorum shell: arguments for the diversity of the nacroprismatic arrangement in molluscs
Summary
Mollusc shells are complex organomineral structures, the arrangement and composition depending on the species. Most studies are dedicated to shells composed of an aragonite nacreous and a calcite prismatic layer, so the nacreous prismatic model based on Pinctada and Atrina–Pinna. Here, we studied the micro‐ and nanostructure, the mineralogy and composition of a nacroprismatic bivalve species: Unio pictorum. The prismatic layer of Unio is aragonite, and the inner structure of the prismatic units strongly differs from those of the calcitic layers. The shape of the prisms varies depending on their growth stage. Th...
Source: Journal of Microscopy - December 14, 2017 Category: Laboratory Medicine Authors: Y. DAUPHIN, G. LUQUET, M. SALOME, L. BELLOT ‐GURLET, J.P. CUIF Tags: Original Article Source Type: research
TOC ‐ Issue Information
(Source: Journal of Microscopy)
Source: Journal of Microscopy - December 13, 2017 Category: Laboratory Medicine Tags: Issue Information Source Type: research
High ‐performance serial block‐face SEM of nonconductive biological samples enabled by focal gas injection‐based charge compensation
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - December 1, 2017 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - December 1, 2017 Category: Laboratory Medicine Source Type: research
High ‐performance serial block‐face SEM of nonconductive biological samples enabled by focal gas injection‐based charge compensation
Summary
A longstanding limitation of imaging with serial block‐face scanning electron microscopy is specimen surface charging. This charging is largely due to the difficulties in making biological specimens and the resins in which they are embedded sufficiently conductive. Local accumulation of charge on the specimen surface can result in poor image quality and distortions. Even minor charging can lead to misalignments between sequential images of the block‐face due to image jitter. Typically, variable‐pressure SEM is used to reduce specimen charging, but this results in a significant reduction to spatial resolution,...
Source: Journal of Microscopy - December 1, 2017 Category: Laboratory Medicine Authors: T. J. DEERINCK, T. M. SHONE, E. A. BUSHONG, R. RAMACHANDRA, S. T. PELTIER, M. H. ELLISMAN Tags: Original Article Source Type: research
Lateral sensitivity in electron probe microanalysis studied by Monte Carlo simulations involving fluorescence enhancements
Summary
In electron probe microanalysis, secondary fluorescence can occur leading to an increase of the volume analysed, degrading the lateral resolution of this technique. An adequate knowledge of the interaction volumes from where the different signals of interest are detected is determinant to estimate the minimum size of the zone that can be characterized. In this work, the size of the signal source volume is surveyed for a wide set of samples at different beam energies. To this aim, the PENELOPE software package was chosen to run Monte Carlo simulations for several experimental situations in order to produce the vario...
Source: Journal of Microscopy - November 27, 2017 Category: Laboratory Medicine Authors: M. PETACCIA, G. CASTELLANO Tags: Original Article Source Type: research
Lateral sensitivity in electron probe microanalysis studied by Monte Carlo simulations involving fluorescence enhancements
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - November 27, 2017 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - November 27, 2017 Category: Laboratory Medicine Source Type: research
Comparative study of image contrast in scanning electron microscope and helium ion microscope
Summary
Images of Ga+‐implanted amorphous silicon layers in a 110 n‐type silicon substrate have been collected by a range of detectors in a scanning electron microscope and a helium ion microscope. The effects of the implantation dose and imaging parameters (beam energy, dwell time, etc.) on the image contrast were investigated. We demonstrate a similar relationship for both the helium ion microscope Everhart‐Thornley and scanning electron microscope Inlens detectors between the contrast of the images and the Ga+ density and imaging parameters. These results also show that dynamic charging effects have a significant ...
Source: Journal of Microscopy - November 20, 2017 Category: Laboratory Medicine Authors: R. O'CONNELL, Y. CHEN, H. ZHANG, Y. ZHOU, D. FOX, P. MAGUIRE, J.J. WANG, C. RODENBURG Tags: Themed Issue Paper Source Type: research
Preface to special issue on Microscopy of Semiconducting Materials 2017 (MSM ‐XX)
(Source: Journal of Microscopy)
Source: Journal of Microscopy - November 20, 2017 Category: Laboratory Medicine Authors: THOMAS WALTHER, LEWYS JONES Tags: Preface Source Type: research
TOC ‐ Issue Information
(Source: Journal of Microscopy)
Source: Journal of Microscopy - November 20, 2017 Category: Laboratory Medicine Tags: Issue Information Source Type: research
Phase retrieval based on coded splitting modulation
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - November 10, 2017 Category: Laboratory Medicine Source Type: research
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Journal of Microscopy, Ahead of Print. (Source: Journal of Microscopy)
Source: Journal of Microscopy - November 10, 2017 Category: Laboratory Medicine Source Type: research
Independent trafficking of flavocytochrome b558 and myeloperoxidase to phagosomes during phagocytosis visualised by energy ‐filtering and energy‐dispersive spectroscopy‐scanning transmission electron microscopy
Summary
When polymorphonuclear leukocytes (PMNs) phagocytose opsonised zymosan particles (OPZ), free radicals and reactive oxygen species (ROS) are formed in the phagosomes. ROS production is mediated by NADPH oxidase (Nox), which transfers electrons in converting oxygen to superoxide (O2−). Nox‐generated O2− is rapidly converted to other ROS. Free radical‐forming secretory vesicles containing the Nox redox center flavocytochrome b558, a membrane protein, and azurophil granules with packaged myeloperoxidase (MPO) have been described. Presuming the probable fusion of these vesicular and granular organelles with phag...
Source: Journal of Microscopy - November 10, 2017 Category: Laboratory Medicine Authors: KEIICHI MORIGUCHI Tags: Letter to the Editor Source Type: research
Probing the effect of polymer molecular weight on penetration into the wood cell wall using polyethylenimine (PEI) as a model compound
Journal of Microscopy, EarlyView. (Source: Journal of Microscopy)
Source: Journal of Microscopy - November 8, 2017 Category: Laboratory Medicine Source Type: research
