Secretome analysis to elucidate metalloprotease ‐dependent ectodomain shedding of glycoproteins during neuronal differentiation
In this study, to elucidate the ectodomain shedding events that occur during neuronal differentiation, we establish a strategy for quantitative secretomics of glycoproteins released from differentiating neuroblastoma cells into culture medium with or without GM6001, a broad‐spectrum metalloprotease inhibitor. Considering that most of transmembrane and secreted proteins are N‐glycosylated, we include a process of N‐glycosylated peptides enrichment as well as isotope tagging in our secretomics workflow. Our results show that differentiating N1E‐115 neurons secrete numerous glycosylated polypeptides in metalloprotease...
Source: Genes to Cells - December 31, 2016 Category: Genetics & Stem Cells Authors: Kazuya Tsumagari, Kyoko Shirakabe, Mayu Ogura, Fuminori Sato, Yasushi Ishihama, Atsuko Sehara ‐Fujisawa Tags: Brief Report Source Type: research
Issue Information
Front cover: In recent years, sirtuin family genes have attracted attention because it has been reported that their activation extended lifespan in some animal species and yeast. Sirtuin family consists of several classes, and the majority of them encode NAD+‐dependent deacetylase. The arrow in this picture is called ‘hamaya,’ which is one of Japanese New Year's good luck charms. It is decorated with two ‘ema’s (wooden votive plaques) likened five‐membered ring of ribose with drawings of a crane and a turtle, common symbols of longevity in Japan. The arrow as a whole is mimicking the structure of NAD+, which is...
Source: Genes to Cells - December 31, 2016 Category: Genetics & Stem Cells Tags: Issue Information Source Type: research
Irradiation with UV ‐C inhibits TNF‐α‐dependent activation of the NF‐κB pathway in a mechanism potentially mediated by reactive oxygen species
Pathways depending on the NF‐κB transcription factor are essential components of cellular response to stress. Plethora of stimuli modulating NF‐κB includes inflammatory signals, ultraviolet radiation (UV) and reactive oxygen species (ROS), yet interference between different factors affecting NF‐κB remains relatively understudied. Here, we aim to characterize the influence of UV radiation on TNF‐α‐induced activity of the NF‐κB pathway. We document inhibition of TNF‐α‐induced activation of NF‐κB and subsequent suppression of NF‐κB‐regulated genes in cells exposed to UV‐C several hours before T...
Source: Genes to Cells - December 14, 2016 Category: Genetics & Stem Cells Authors: Katarzyna Szoltysek, Anna Walaszczyk, Patryk Janus, Marek Kimmel, Piotr Widlak Tags: Original Article Source Type: research
Coupling of a voltage ‐gated Ca2+ channel homologue with a plasma membrane H+‐ATPase in yeast
In this report, we suggest that Mid1 physically interacts with Pma1, a plasma membrane H+‐ATPase and that the H+‐pumping activity of Pma1 is important for the activation of the Cch1/Mid1 calcium channel. (Source: Genes to Cells)
Source: Genes to Cells - December 8, 2016 Category: Genetics & Stem Cells Authors: Toshihiko Cho, Aya Ishii ‐Kato, Yuko Fukata, Yoshitaka Nakayama, Kazuko Iida, Masaki Fukata, Hidetoshi Iida Tags: Original Article Source Type: research
CK2 phospho ‐independent assembly of the Tel2‐associated stress‐signaling complexes in Schizosaccharomyces pombe
An evolutionarily conserved protein Tel2 regulates a variety of stress signals. In mammals, TEL2 associates with TTI1 and TTI2 to form the Triple T (TTT: TEL2‐TTI1‐TTI2) complex as well as with all the phosphatidylinositol 3‐kinase‐like kinases (PIKKs) and the R2TP (Ruvbl1‐Ruvbl2‐Tah1‐Pih1 in budding yeast)/prefoldin‐like complex that associates with HSP90. The phosphorylation of TEL2 by casein kinase 2 (CK2) enables direct binding of PIHD1 (mammalian Pih1) to TEL2 and is important for the stability and the functions of PIKKs. However, the regulatory mechanisms of Tel2 in fission yeast Schizosaccharomyces p...
Source: Genes to Cells - December 8, 2016 Category: Genetics & Stem Cells Authors: Haruna Inoue, Shizuka Sugimoto, Yumiko Takeshita, Miho Takeuchi, Mitsuko Hatanaka, Koji Nagao, Takeshi Hayashi, Aya Kokubu, Mitsuhiro Yanagida, Junko Kanoh Tags: Original Article Source Type: research
