Roles of the third Ig ‐like domain of Necl‐5/PVR and the fifth Ig‐like domain of the PDGF receptor in its signaling
Genes to Cells,Volume 23, Issue 3, Page 214-224, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - February 12, 2018 Category: Genetics & Stem Cells Source Type: research
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Genes to Cells,Volume 23, Issue 3, Page 234-240, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - February 12, 2018 Category: Genetics & Stem Cells Source Type: research
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Genes to Cells,Volume 23, Issue 3, Page 185-199, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - February 12, 2018 Category: Genetics & Stem Cells Source Type: research
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Genes to Cells,Volume 23, Issue 3, Page 214-224, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - February 12, 2018 Category: Genetics & Stem Cells Source Type: research
Requirement of the F ‐actin‐binding activity of l‐afadin for enhancing the formation of adherens and tight junctions
The apical junctional complex consists of adherens junctions (AJs) and tight junctions (TJs) in polarized epithelial cells, which are attached to each other to form a sheet. Actin filaments (F‐actin) are associated with AJs and TJs and required for the formation and maintenance of this complex. l‐Afadin is an F‐actin‐binding protein, which is localized at AJs through binding to the cell adhesion molecule nectin, and regulates the formation of AJs and TJs. However, the role of the F‐actin‐binding activity of l‐afadin for the formation of the apical junctional complex remains unknown. We generated here the cult...
Source: Genes to Cells - February 12, 2018 Category: Genetics & Stem Cells Authors: Shotaro Sakakibara, Tomohiko Maruo, Muneaki Miyata, Kiyohito Mizutani, Yoshimi Takai Tags: ORIGINAL ARTICLE Source Type: research
Wingless signaling regulates winner/loser status in Minute cell competition
Cells heterozygously mutant for a ribosomal protein gene, called Minute/+ mutants, are eliminated from epithelium by cell competition when surrounded by wild‐type cells. Whereas several factors that regulate Minute cell competition have been identified, the mechanisms how winner/loser status is determined and thereby triggers cell competition are still elusive. To address this, we established two assay systems for Minute cell competition, namely (i) the CORE (competitive elimination of RpS3‐RNAi‐expressing cells) system in which RpS3‐RNAi‐expressing wing pouch cells are eliminated from wild‐type wing disc and (...
Source: Genes to Cells - February 12, 2018 Category: Genetics & Stem Cells Authors: Nanami Akai, Tatsushi Igaki, Shizue Ohsawa Tags: BRIEF REPORT Source Type: research
Unexpected heterogeneity derived from Cas9 ribonucleoprotein ‐introduced clonal cells at the HPRT1 locus
Genes to Cells, EarlyView. (Source: Genes to Cells)
Source: Genes to Cells - February 9, 2018 Category: Genetics & Stem Cells Source Type: research
Mutations at multiple CDK phosphorylation consensus sites on Cdt2 increase the affinity of CRL4Cdt2 for PCNA and its ubiquitination activity in S phase
Genes to Cells,Volume 23, Issue 3, Page 200-213, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - February 9, 2018 Category: Genetics & Stem Cells Source Type: research
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Genes to Cells, Ahead of Print. (Source: Genes to Cells)
Source: Genes to Cells - February 9, 2018 Category: Genetics & Stem Cells Source Type: research
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Genes to Cells,Volume 23, Issue 3, Page 200-213, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - February 9, 2018 Category: Genetics & Stem Cells Source Type: research
Mutations at multiple CDK phosphorylation consensus sites on Cdt2 increase the affinity of CRL4Cdt2 for PCNA and its ubiquitination activity in S phase
CRL4Cdt2 ubiquitin ligase plays an important role maintaining genome integrity during the cell cycle. A recent report suggested that Cdk1 negatively regulates CRL4Cdt2 activity through phosphorylation of its receptor, Cdt2, but the involvement of phosphorylation remains unclear. To address this, we mutated all CDK consensus phosphorylation sites located in the C‐terminal half region of Cdt2 (Cdt2‐18A) and examined the effect on substrate degradation. We show that both cyclinA/Cdk2 and cyclinB/Cdk1 phosphorylated Cdt2 in vitro and that phosphorylation was reduced by the 18A mutation both in vitro and in vivo. The 18A mu...
Source: Genes to Cells - February 9, 2018 Category: Genetics & Stem Cells Authors: Kohei Nukina, Akiyo Hayashi, Yasushi Shiomi, Kaoru Sugasawa, Motoaki Ohtsubo, Hideo Nishitani Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
Front cover: In Japan, people cerebrate Setsubun, the day before the lunar calendrical beginning of spring, around 3rd of February every year. People decorate sardine heads and holly leaves, put soy beans in a square wooden measuring box (‘masu’), and scatter the beans to expel the bad spirits. Putting not only ordinary soybeans, but also other kinds of beans, we expressed a scene of the inside of a cell in the masu just like a bonsai. The real inside of the cells isn’t sparse, but very much crowded, like this masu, with cellular organelles such as the nucleus, mitochondria, secretory vesicles and Golgi apparatus and...
Source: Genes to Cells - February 8, 2018 Category: Genetics & Stem Cells Tags: ISSUE INFORMATION Source Type: research
Effect of a clockwise ‐locked deletion in FliG on the FliG ring structure of the bacterial flagellar motor
Genes to Cells,Volume 23, Issue 3, Page 241-247, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - February 6, 2018 Category: Genetics & Stem Cells Source Type: research
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Genes to Cells,Volume 23, Issue 3, Page 241-247, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - February 6, 2018 Category: Genetics & Stem Cells Source Type: research
Atomic force microscopy analysis of SasA ‐KaiC complex formation involved in information transfer from the KaiABC clock machinery to the output pathway in cyanobacteria
The cyanobacterial clock oscillator is composed of three clock proteins: KaiA, KaiB and KaiC. SasA, a KaiC‐binding EnvZ‐like orthodox histidine kinase involved in the main clock output pathway, exists mainly as a trimer (SasA3mer) and occasionally as a hexamer (SasA6mer) in vitro. Previously, the molecular mass of the SasA‐KaiCDD complex, where KaiCDD is a mutant KaiC with two Asp substitutions at the two phosphorylation sites, has been estimated by gel‐filtration chromatography to be larger than 670 kDa. This value disagrees with the theoretical estimation of 480 kDa for a SasA3mer‐KaiC hexamer (KaiC6mer) comp...
Source: Genes to Cells - February 1, 2018 Category: Genetics & Stem Cells Authors: Reiko Murakami, Hitomi Hokonohara, Dock ‐Chil Che, Tomoji Kawai, Takuya Matsumoto, Masahiro Ishiura Tags: ORIGINAL ARTICLE Source Type: research
