Dullard deficiency causes hemorrhage in the adult ovarian follicles
In mammals, the ovarian follicles are regulated at least in part by bone morphogenetic protein (BMP) family members. Dullard (also known as Ctdnep1) gene encodes a phosphatase that suppresses BMP signaling by inactivating or degrading BMP receptors. Here we report that the Col1a1‐Cre‐induced Dullard mutant mice displayed hemorrhagic ovarian cysts, with red blood cells accumulated in the follicles, resulting in infertility. Cells expressing Cre driven by Col1a1 2.3‐kb promoter and their descendants were found in granulosa cells in the ovary and in Sertoli cells in the testis. DullardmRNA was localized to granulosa cel...
Source: Genes to Cells - February 1, 2018 Category: Genetics & Stem Cells Authors: Tadayoshi Hayata, Masahiko Chiga, Yoichi Ezura, Makoto Asashima, Hidetaka Katabuchi, Ryuichi Nishinakamura, Masaki Noda Tags: ORIGINAL ARTICLE Source Type: research
Phosphorylation of N ‐terminal regions of REV‐ERBs regulates their intracellular localization
In this study, we found that the highly homologous N‐terminal regions of REV‐ERBα and REV‐ERBβ determined both their own CK1‐catalyzed phosphorylation and the cytoplasmic accumulation of each hyperphosphorylated form. Of the homologous N‐terminal regions, three serine‐rich clusters in REV‐ERBβ are required for the phosphorylation and cytoplasmic localization. Our results indicate that the REV‐ERBs phosphorylation by CK1 plays a key role in their subcellular localization, thereby controlling the timings of the transcriptional activation and inhibition of Bmal1.
We found that the highly homologous N‐ter...
Source: Genes to Cells - February 1, 2018 Category: Genetics & Stem Cells Authors: Yuki Ohba, Hajime Tei Tags: ORIGINAL ARTICLE Source Type: research
The periplasmic disordered domain of RodZ promotes its self ‐interaction in Escherichia coli
Rod shape of bacterial cells such as Escherichia coli is mainly regulated by a supramolecular complex called elongasome including MreB actin. Deletion of the mreB gene in rod‐shaped bacterium E. coli results in round‐shaped cells. RodZ was isolated as a determinant of rod shape in E. coli, Caulobacter crescentus and Bacillus subtilis and it has been shown to be an interaction partner and a regulator of assembly of MreB through its cytoplasmic domain. As opposed to functions of the N‐terminal cytoplasmic domain of RodZ, functions of the C‐terminal periplasmic domain including a disordered region are still unclear....
Source: Genes to Cells - February 1, 2018 Category: Genetics & Stem Cells Authors: Ryosuke Ikebe, Yuri Kuwabara, Taiki Chikada, Hironori Niki, Daisuke Shiomi Tags: ORIGINAL ARTICLE Source Type: research
Transposable elements shape the human proteome landscape via formation of cis ‐acting upstream open reading frames
This study shows that transposons have potentially been shaping the human proteome landscape via cis‐acting uORF creation. (Source: Genes to Cells)
Source: Genes to Cells - February 1, 2018 Category: Genetics & Stem Cells Authors: Shohei Kitano, Hikaru Kurasawa, Yasunori Aizawa Tags: ORIGINAL ARTICLE Source Type: research
Aggregatibacter actinomycetemcomitans infection causes DNA double ‐strand breaks in host cells
Periodontal disease, an inflammatory disease, is caused by infection with periodontal pathogens. Long‐term periodontal disease increases the risk of oral carcinogenesis. Similar to other peptic cancers, oral carcinogenesis also requires multiple genome instabilities; however, the risk factors related to the accumulation of genome instabilities are poorly understood. Here, we suggested that specific periodontal pathogens may increase the risk of genome instability. Accordingly, we screened several periodontal pathogens based on the ability to induce DNA double‐strand breaks (DSBs) in host cells. We found that Aggregatib...
Source: Genes to Cells - February 1, 2018 Category: Genetics & Stem Cells Authors: Rie Teshima, Katsuhiro Hanada, Junko Akada, Kenji Kawano, Yoshio Yamaoka Tags: ORIGINAL ARTICLE Source Type: research
Roles of the third Ig ‐like domain of Necl‐5/PVR and the fifth Ig‐like domain of the PDGF receptor in its signaling
The immunoglobulin (Ig)‐like cell adhesion molecule nectin‐like molecule (Necl)‐5/poliovirus receptor is up‐regulated in many types of cancer cells and implicated in their abnormally enhanced cell proliferation and movement. We previously showed that Necl‐5 cis‐interacts with the platelet‐derived growth factor (PDGF) receptor β through the extracellular region and enhances its signaling. Although this cis‐interaction does not affect the PDGF‐induced tyrosine phosphorylation of the receptor, the interaction of the cytoplasmic region of Necl‐5 with sprouty2 and the regulation of its activity are required...
Source: Genes to Cells - February 1, 2018 Category: Genetics & Stem Cells Authors: Yuki Ueda, Shin Kedashiro, Masahiro Maruoka, Kiyohito Mizutani, Yoshimi Takai Tags: ORIGINAL ARTICLE Source Type: research
Unexpected heterogeneity derived from Cas9 ribonucleoprotein ‐introduced clonal cells at the HPRT1 locus
In this study, we investigated how the timing for single‐cell cloning of Cas9 RNP‐transfected cells affected the heterogeneity of the resultant clones. We carried out transfection of Cas9 RNPs targeting several loci in the HPRT1 gene in HCT116 cells, followed by single‐cell cloning at 24, 48, 72 hr and 1 week post‐transfection. After approximately 3 weeks of incubation, the clonal cells were collected and genotyped by high‐resolution microchip electrophoresis and Sanger sequencing. Unexpectedly, long‐term incubation before single‐cell cloning resulted in highly heterogeneous clones. We used a lipofection m...
Source: Genes to Cells - February 1, 2018 Category: Genetics & Stem Cells Authors: Tetsushi Sakuma, Keiji Mochida, Shota Nakade, Toru Ezure, Sachi Minagawa, Takashi Yamamoto Tags: ORIGINAL ARTICLE Source Type: research
RBMX is a component of the centromere noncoding RNP complex involved in cohesion regulation
Genes to Cells,Volume 23, Issue 3, Page 172-184, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 31, 2018 Category: Genetics & Stem Cells Source Type: research
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Genes to Cells,Volume 23, Issue 3, Page 172-184, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 31, 2018 Category: Genetics & Stem Cells Source Type: research
Tumor necrosis factor ‐α regulates human follicular dendritic cell‐secreted protein gene transcription in gingival epithelial cells
Genes to Cells,Volume 23, Issue 3, Page 161-171, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 22, 2018 Category: Genetics & Stem Cells Source Type: research
Preferable in vitro condition for maintaining faithful DNA methylation imprinting in mouse embryonic stem cells
Genes to Cells,Volume 23, Issue 3, Page 146-160, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 22, 2018 Category: Genetics & Stem Cells Source Type: research
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Genes to Cells,Volume 23, Issue 3, Page 161-171, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 22, 2018 Category: Genetics & Stem Cells Source Type: research
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Genes to Cells,Volume 23, Issue 3, Page 146-160, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 22, 2018 Category: Genetics & Stem Cells Source Type: research
Preferable in vitro condition for maintaining faithful DNA methylation imprinting in mouse embryonic stem cells
Epigenetic properties of cultured embryonic stem cells (ESCs), including DNA methylation imprinting, are important because they affect the developmental potential. Here, we tested a variety of culture media, including knockout serum replacement (KSR) and fetal bovine serum (FBS) with or without inhibitors of Gsk3β and Mek1/2 (2i) at various time points. In addition to the previously known passage‐dependent global changes, unexpected dynamic DNA methylation changes occurred in both maternal and paternal differentially methylated regions: under the widely used condition of KSR with 2i, a highly hypomethylated state occurr...
Source: Genes to Cells - January 22, 2018 Category: Genetics & Stem Cells Authors: Jiyoung Lee, Ayumi Matsuzawa, Hirosuke Shiura, Akito Sutani, Fumitoshi Ishino Tags: ORIGINAL ARTICLE Source Type: research
MicroRNA ‐664a‐5p promotes neuronal differentiation of SH‐SY5Y cells
Genes to Cells,Volume 23, Issue 3, Page 225-233, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 17, 2018 Category: Genetics & Stem Cells Source Type: research
