Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass
Genes to Cells,Volume 23, Issue 3, Page 136-145, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 17, 2018 Category: Genetics & Stem Cells Source Type: research
---
Genes to Cells,Volume 23, Issue 3, Page 225-233, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 17, 2018 Category: Genetics & Stem Cells Source Type: research
---
Genes to Cells,Volume 23, Issue 3, Page 136-145, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 17, 2018 Category: Genetics & Stem Cells Source Type: research
Issue Information
Front cover: At Japanese shrines during New Year's holidays, you can find such dedicatory barrels of sake as in this picture. Looking closely at their labels, you will see the 3D structure, drawn on them, of green fluorescent protein, chaperonin, proteasome, aquaporin and sucrose porin, all of which have barrel‐like tertiary or quaternary structure. Designed by TRAIS Co., Ltd. (Kobe, Japan). (Source: Genes to Cells)
Source: Genes to Cells - January 16, 2018 Category: Genetics & Stem Cells Tags: ISSUE INFORMATION Source Type: research
Lem2 is retained at the nuclear envelope through its interaction with Bqt4 in fission yeast
Genes to Cells,Volume 23, Issue 3, Page 122-135, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 2, 2018 Category: Genetics & Stem Cells Source Type: research
---
Genes to Cells,Volume 23, Issue 3, Page 122-135, March 2018. (Source: Genes to Cells)
Source: Genes to Cells - January 2, 2018 Category: Genetics & Stem Cells Source Type: research
Efficient identification of compounds suppressing protein precipitation via solvent screening using serial deletion mutants of the target protein
In this study, the C‐terminal chemokine receptor‐binding region of the cytoplasmic protein, FROUNT (FNT‐C), which mediates intracellular signals leading to leukocyte migration, was subjected to the multicomponent screening. In total, 192 solution conditions were tested, using eight terminal deletion mutants of FNT‐C. We identified five solvent conditions that solubilized four or five mutants of FNT‐C, and the compounds in the screened solvents were then, respectively, assessed in terms of their solubilization ability. The best compound for solubilizing FNT‐C was 1,6‐hexanediol. Indeed, 1,6‐hexanediol bound ...
Source: Genes to Cells - January 2, 2018 Category: Genetics & Stem Cells Authors: Kaori Yunoki, Sosuke Yoshinaga, Mitsuhiro Takeda, Ryohei Nagano, Yusuke Tsuchiya, Akihiro Sonoda, Tatsuichiro Tsuji, Makoto Hirakane, Etsuko Toda, Yuya Terashima, Kouji Matsushima, Hiroaki Terasawa Tags: ORIGINAL ARTICLE Source Type: research
Effect of a clockwise ‐locked deletion in FliG on the FliG ring structure of the bacterial flagellar motor
FliG is a rotor protein of the bacterial flagellar motor. FliG consists of FliGN, FliGM and FliGC domains. Intermolecular FliGM–FliGC interactions promote FliG ring formation on the cytoplasmic face of the MS ring. A conformational change in HelixMC connecting FliGM and FliGC is responsible for the switching between the counterclockwise (CCW) and clockwise (CW) rotational states of the FliG ring. However, it remains unknown how it occurs. Here, we carried out in vivo disulfide cross‐linking experiments to see the effect of a CW‐locked deletion (∆PAA) in FliG on the FliG ring structure in Salmonella enterica. Higher...
Source: Genes to Cells - January 1, 2018 Category: Genetics & Stem Cells Authors: Miki Kinoshita, Keiichi Namba, Tohru Minamino Tags: BRIEF REPORT Source Type: research
RBMX is a component of the centromere noncoding RNP complex involved in cohesion regulation
Satellite I RNA, a noncoding (nc)RNA transcribed from repetitive regions in human centromeres, binds to Aurora kinase B and forms a ncRNP complex required for chromosome segregation. To examine its function in this process, we purified satellite I ncRNP complex from nuclear extracts prepared from asynchronized or mitotic (M) phase‐arrested HeLa cells and then carried out LC/MS to identify proteins bound to satellite I RNA. RBMX (RNA‐binding motif protein, X‐linked), which was isolated from M phase‐arrested cells, was selected for further characterization. We found that RBMX associates with satellite I RNA only duri...
Source: Genes to Cells - January 1, 2018 Category: Genetics & Stem Cells Authors: Yukiko Cho, Takashi Ideue, Megumi Nagayama, Norie Araki, Tokio Tani Tags: ORIGINAL ARTICLE Source Type: research
Tumor necrosis factor ‐α regulates human follicular dendritic cell‐secreted protein gene transcription in gingival epithelial cells
Follicular dendritic cell‐secreted protein (FDC‐SP) is a secreted protein expressed in follicular dendritic cells, periodontal ligament and junctional epithelium. To elucidate the transcriptional regulation of the human FDC‐SP gene by tumor necrosis factor‐α (TNF‐α), we conducted real‐time PCR, Western blotting, transient transfection analyses with chimeric constructs of the FDC‐SP gene promoter linked to a luciferase reporter gene, gel mobility shift and chromatin immunoprecipitation assays using Ca9‐22 gingival epithelial cells. TNF‐α (10 ng/ml) induced FDC‐SP mRNA and protein levels at 3 hr and ...
Source: Genes to Cells - January 1, 2018 Category: Genetics & Stem Cells Authors: Yasunobu Iwai, Keisuke Noda, Mizuho Yamazaki, Ayako Kato, Masaru Mezawa, Hideki Takai, Yohei Nakayama, Yorimasa Ogata Tags: ORIGINAL ARTICLE Source Type: research
Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass
This study showed that Areg mRNA expression was significantly up‐regulated in obese adipose tissues and over‐expression of Areg in white adipose tissue caused less adipose tissue mass.
Areg mRNA expression was significantly up‐regulated in mouse obese adipose tissues, and over‐expression of Areg in white adipose tissue caused less adipose tissue mass. (Source: Genes to Cells)
Source: Genes to Cells - January 1, 2018 Category: Genetics & Stem Cells Authors: Bo Yang, Takahiro Kumoto, Takeshi Arima, Minako Nakamura, Yohei Sanada, Thanutchaporn Kumrungsee, Yusuke Sotomaru, Masayuki Shimada, Noriyuki Yanaka Tags: ORIGINAL ARTICLE Source Type: research
MicroRNA ‐664a‐5p promotes neuronal differentiation of SH‐SY5Y cells
In this study, we have attempted to identify novel miRNAs related to neuronal differentiation via microarray analysis in the human neuronal differentiation model neuroblastoma SH‐SY5Y cells. We identified 15 up‐regulated and eight down‐regulated miRNAs in SH‐SY5Y cells treated with all‐trans retinoic acid to induce differentiation. We further showed that one of the up‐regulated miRNAs, miR‐664a‐5p, promoted neuronal differentiation of SH‐SY5Y cells. These findings enhance our understanding of the miRNAs involved in the process of neurogenesis and, in particular, highlight an important role of miR‐664a...
Source: Genes to Cells - January 1, 2018 Category: Genetics & Stem Cells Authors: Kazunori Watanabe, Ryuhei Yamaji, Takashi Ohtsuki Tags: BRIEF REPORT Source Type: research
Disruption of circadian clockwork in in vivo reprogramming ‐induced mouse kidney tumors
The circadian clock, which regulates cellular physiology, such as energy metabolism, resides in each cell level throughout the body. Recently, it has been elucidated that the cellular circadian clock is closely linked with cellular differentiation. Moreover, the misregulation of cellular differentiation in mouse embryonic stem cells (ESCs) induced abnormally differentiated cells with impaired circadian clock oscillation, concomitant with the post‐transcriptional suppression of CLOCK proteins. Here, we show that the circadian molecular oscillation is disrupted in dysdifferentiation‐mediated mouse kidney tumors induced b...
Source: Genes to Cells - December 22, 2017 Category: Genetics & Stem Cells Authors: Munehiro Ohashi, Yasuhiro Umemura, Nobuya Koike, Yoshiki Tsuchiya, Yutaka Inada, Hitomi Watanabe, Tomoko Tanaka, Yoichi Minami, Osamu Ukimura, Tsuneharu Miki, Tatsuro Tajiri, Gen Kondoh, Yasuhiro Yamada, Kazuhiro Yagita Tags: ORIGINAL ARTICLE Source Type: research
Contribution of neuraminidase 3 to the differentiation of induced regulatory T cells
Neuraminidase family enzymes that hydrolyze the terminal sialic acid linkage in biomolecules are involved in various immune responses. We previously showed that Th1 and Th2 cells differentially express several neuraminidases. Herein, the expression of neuraminidases in induced regulatory T (iTreg) cells was investigated in comparison with that in other T‐cell subsets. Contrary to the tendency toward higher neuraminidase 1 mRNA expression in in vitro‐differentiated Th2 cells, compared to Th1, Th17 and iTreg cells, we observed significantly higher expression of neuraminidase 3 (Neu3) in iTreg cells. Furthermore, the expr...
Source: Genes to Cells - December 22, 2017 Category: Genetics & Stem Cells Authors: Osamu Kaminuma, Shigeki Katoh, Taeko Miyagi, Nobumasa Watanabe, Noriko Kitamura, Tomoe Nishimura, Mayumi Saeki, Akio Mori, Takachika Hiroi Tags: BRIEF REPORT Source Type: research
Volume Contents
(Source: Genes to Cells)
Source: Genes to Cells - December 7, 2017 Category: Genetics & Stem Cells Tags: Volume Contents Source Type: research
