Developmental gene expression patterns in the brain and liver of Xenopus tropicalis during metamorphosis climax
In this study, we examined the developmental gene expression profiles in the brain and liver ofXenopus tropicalis during metamorphosis climax and compared them to the respective gene expression profiles of newborn rodents. Many upregulated genes identified in the tadpole brain during metamorphosis are also upregulated in the rodent brain during the first three postnatal weeks when the TH surge occurs. The upregulation of some genes in the brain was inhibited inthyroid hormone receptor α (TR α) knockout tadpoles but not inTR β–knockout tadpoles, implying that brain metamorphosis is mainly mediated byTR α. The expressi...
Source: Genes to Cells - November 4, 2018 Category: Genetics & Stem Cells Authors: Yoshio Yaoita,
Keisuke Nakajima Tags: ORIGINAL ARTICLE Source Type: research
Accumulation of the myosin ‐II‐spectrin complex plays a positive role in apical extrusion of Src‐transformed epithelial cells
In this study, we demonstrate that myosin ‐II and beta‐spectrin accumulate in Src‐transformed cells when they are surrounded by normal epithelial cells. Knock‐down of the heavy chains of myosin‐II or beta‐spectrin substantially diminishes apical extrusion of Src cells. Collectively, these results indicate that accumulation of th e myosin‐II‐spectrin complex plays a positive role in elimination of Src‐transformed epithelial cells. AbstractAt the initial stage of carcinogenesis, transformation occurs in single cells within the epithelium. Recent studies have revealed that the newly emerging transformed cell...
Source: Genes to Cells - November 4, 2018 Category: Genetics & Stem Cells Authors: Mikio Takagi,
Masaya Ikegawa,
Takashi Shimada,
Susumu Ishikawa,
Mihoko Kajita,
Takeshi Maruyama,
Tomoko Kamasaki,
Yasuyuki Fujita Tags: BRIEF REPORT Source Type: research
Issue Information
Front cover: When drawing a saturation binding curve of two proteins, there is a way of plotting the concentration of the free form of one of the pair on X axis, and the ratio of the other that is forming complex on Y axis. In this case, the slope of the curve is small if their affinity is low (i.e. large dissociation constant (Kd)); however, the slope is large if the affinity is high (i.e. smallKd). In this drawing, two flocks of wild geese are flying along two line ‐shaped clouds that are likened to saturation binding curves. The geese along the lower, gentle‐slope cloud are flying apart as if their affinity is low. ...
Source: Genes to Cells - November 4, 2018 Category: Genetics & Stem Cells Tags: ISSUE INFORMATION Source Type: research
Flow cytometric quantitation of EpCAM ‐positive extracellular vesicles by immunomagnetic separation and phospholipid staining method
Extracellular vesicles (EV) in cultured cell supernatant are quantitated by immunomagnetic separation and phospholipid staining (IMS ‐PS) method developed in the paper. Several improvements are made (A), appropriate amount of the bead is determined (B), and titrations of OVCAR3 EV and A431 EV show linear response between input EV sample volume and output counts (C). Colon‐26 EV are non‐human control. AbstractExtracellular vesicles (EV) have attracted attention as circulating biomarkers for many diseases, particularly cancer. Conventional immunofluorescence staining has been used for the detection of target antigens o...
Source: Genes to Cells - November 4, 2018 Category: Genetics & Stem Cells Authors: Masashi Takao,
Yutaka Nagai,
Masami Ito,
Tetsuhiko Ohba Tags: ORIGINAL ARTICLE Source Type: research
FEAT enhances INSL3 expression in testicular Leydig cells
FEAT(METTL13) is expressed in fetal and adult Leydig cells in the testis. FEAT ‐silenced MA‐10 Leydig cells showed diminished INSL3 protein. A maleMettl13+/ − mouse exhibited bilateral intraabdominal cryptorchidism and markedly decreased INSL3 expression in Leydig cells. AbstractFEAT, the protein encoded by methyltransferase ‐like 13 (METTL13), is aberrantly upregulated in most human cancers and potently drives tumorigenesis in vivo; however, its role in normal tissues remains elusive. Immunoblotting has displayed weak FEAT expression in normal human tissues, including the testis. Here, we found that FEAT is expres...
Source: Genes to Cells - November 4, 2018 Category: Genetics & Stem Cells Authors: Yan Li,
Kyosuke Kobayashi,
Kosho Murayama,
Kohichi Kawahara,
Yuichi Shima,
Akira Suzuki,
Kenzaburo Tani,
Atsushi Takahashi Tags: ORIGINAL ARTICLE Source Type: research
Integrin β1 activity is required for cardiovascular formation in zebrafish
In this study, we investigated integrin β1 function in endothelial cells of zebrafish. Anintegrin β1b mutant zebrafish exhibited morphological abnormalities in blood vessel formation, cephalic hemorrhage and a decreased responsiveness to tactile stimulation during development. To determine the role of integrin β1b in vascular formation, we developed a Gal4/UAS‐mediated conditional inactivation of integrin β1 by expressing the cytoplasmic region of integrin β1 that acts as a dominant‐negative (DN) isoform. Expression of integrin β1 DN in endothelial cells induced blood vessel abnormalities as inintegrin β1b mutan...
Source: Genes to Cells - November 4, 2018 Category: Genetics & Stem Cells Authors: Atsuo Iida,
Zi Wang,
Hiromi Hirata,
Atsuko Sehara ‐Fujisawa Tags: ORIGINAL ARTICLE Source Type: research
Regulation of LRG1 expression by RNA ‐binding protein Puf5 in the budding yeast cell wall integrity pathway
Puf5 functions in the yeast cell wall integrity pathway by regulatingLRG1 expression in a deadenylase ‐independent manner, and Puf6 is involved in the Ccr4‐ and Puf5‐mediated regulation of cell growth through association with Rpl43. AbstractThe PUF RNA ‐binding protein Puf5 is involved in regulation of the cell wall integrity (CWI) pathway in yeast. Puf5 negatively regulates expression ofLRG1 mRNA, encoding for a GTPase ‐activating protein for Rho1 small GTPase. Here, we further analyzed the effect of Puf5 onLRG1 expression, together with Ccr4 and Pop2 deadenylases, Dhh1 decapping activator, and other PUF protei...
Source: Genes to Cells - October 31, 2018 Category: Genetics & Stem Cells Authors: Nguyen Thi Minh Viet,
Duong Long Duy,
Kazuhiro Saito,
Kaoru Irie,
Yasuyuki Suda,
Tomoaki Mizuno,
Kenji Irie Tags: ORIGINAL ARTICLE Source Type: research
Regulation of LRG1 expression by RNA ‐binding protein Puf5 in the budding yeast cell wall integrity pathway
Genes to Cells, EarlyView. (Source: Genes to Cells)
Source: Genes to Cells - October 31, 2018 Category: Genetics & Stem Cells Authors: Nguyen Thi Minh Viet,
Duong Long Duy,
Kazuhiro Saito,
Kaoru Irie,
Yasuyuki Suda,
Tomoaki Mizuno,
Kenji Irie Source Type: research
Genetic suppression of CRMP2 phosphorylation improves outcome in MPTP ‐induced Parkinson's model mice
Genes to Cells,Volume 0, Issue ja, -Not available-. (Source: Genes to Cells)
Source: Genes to Cells - October 30, 2018 Category: Genetics & Stem Cells Authors: Kentaro Togashi,
Masaya Hasegawa,
Jun Nagai,
Aine Tonouchi,
Daiki Masukawa,
Kenneth Hensley,
Yoshio Goshima,
Toshio Ohshima Source Type: research
Genetic suppression of CRMP2 phosphorylation improves outcome in MPTP ‐induced Parkinson's model mice
This article is protected by copyright. All rights reserved. (Source: Genes to Cells)
Source: Genes to Cells - October 29, 2018 Category: Genetics & Stem Cells Authors: Kentaro Togashi,
Masaya Hasegawa,
Jun Nagai,
Aine Tonouchi,
Daiki Masukawa,
Kenneth Hensley,
Yoshio Goshima,
Toshio Ohshima Tags: Original Article Source Type: research
Oral administration of antibiotics results in fecal occult bleeding due to metabolic disorders and defective proliferation of the gut epithelial cell in mice
This article is protected by copyright. All rights reserved. (Source: Genes to Cells)
Source: Genes to Cells - October 24, 2018 Category: Genetics & Stem Cells Authors: Akira Sonoda,
Naganori Kamiyama,
Sotaro Ozaka,
Yoshiko Gendo,
Takashi Ozaki,
Haruna Hirose,
Kaori Noguchi,
Benjawan Saechue,
Nozomi Sachi,
Kumiko Sakai,
Kazuhiro Mizukami,
Shinya Hidano,
Kazunari Murakami,
Takashi Kobayashi Tags: Original Article Source Type: research
Oral administration of antibiotics results in fecal occult bleeding due to metabolic disorders and defective proliferation of the gut epithelial cell in mice
Genes to Cells,Volume 0, Issue ja, -Not available-. (Source: Genes to Cells)
Source: Genes to Cells - October 24, 2018 Category: Genetics & Stem Cells Authors: Akira Sonoda,
Naganori Kamiyama,
Sotaro Ozaka,
Yoshiko Gendo,
Takashi Ozaki,
Haruna Hirose,
Kaori Noguchi,
Benjawan Saechue,
Nozomi Sachi,
Kumiko Sakai,
Kazuhiro Mizukami,
Shinya Hidano,
Kazunari Murakami,
Takashi Kobayashi Source Type: research
MicroRNA ‐342 inhibits tumor growth via targeting chemokine CXCL12 involved in macrophages recruitment/activation
This article is protected by copyright. All rights reserved. (Source: Genes to Cells)
Source: Genes to Cells - October 22, 2018 Category: Genetics & Stem Cells Authors: Yijun Tian,
Sayaka Matusi,
Maki Touma,
Qiong Wu,
Kenkichi Sugimoto Tags: Original Article Source Type: research
MicroRNA ‐342 inhibits tumor growth via targeting chemokine CXCL12 involved in macrophages recruitment/activation
Genes to Cells,Volume 0, Issue ja, -Not available-. (Source: Genes to Cells)
Source: Genes to Cells - October 22, 2018 Category: Genetics & Stem Cells Authors: Yijun Tian,
Sayaka Matusi,
Maki Touma,
Qiong Wu,
Kenkichi Sugimoto Source Type: research
Issue Information
Genes to Cells,Volume 23, Issue 10, Page 803-807, October 2018. (Source: Genes to Cells)
Source: Genes to Cells - October 15, 2018 Category: Genetics & Stem Cells Source Type: research
