Proteomics and its impact on food allergy diagnosis
Publication date: Available online 2 April 2016 Source:EuPA Open Proteomics Author(s): Karin Hoffmann-Sommergruber Food allergies are a relevant health problem and symptoms range from mild to severe life-threatening reactions. With the help of up to date proteomics the causative food allergens can be identified from individual food sources. A short overview on the application of proteomics to assess the physicochemical properties of food allergens is presented. Graphical abstract (Source: EuPA Open Proteomics)
Source: EuPA Open Proteomics - April 2, 2016 Category: Bioinformatics Source Type: research
Top-down proteomic characterization of daoy medulloblastoma tumor cell line
Publication date: Available online 1 April 2016 Source:EuPA Open Proteomics Author(s): Claudia Martelli, Luca D’Angelo, Marta Barba, Mirko Baranzini, Ilaria Inserra, Federica Iavarone, Federica Vincenzoni, Gianpiero Tamburrini, Luca Massimi, Concezio Di Rocco, Massimo Caldarelli, IreneMessana, Fabrizio Michetti, Massimo Castagnola, Wanda Lattanzi, Claudia Desiderio The proteome of the DAOY medulloblastoma cell line has been investigated by an LC-MS top-down platform. This approach, unlike the bottom-up one, allows identifying proteins and peptides in their intact forms, and disclosing...
Source: EuPA Open Proteomics - April 1, 2016 Category: Bioinformatics Source Type: research
Sprague Dawley rats: a model of successful heart aging
Publication date: Available online 1 April 2016 Source:EuPA Open Proteomics Author(s): Daniele Capitanio, Roberta Leone, Chiara Fania, Enrica Torretta, Cecilia Gelfi Aging is a universal phenomenon involving the whole body and characterized by the dysfunction of metabolic and physiological processes, leading to cardiovascular defects and heart failure. The proteomic profiles of Sprague Dawley rat hearts of 6, 22 and 30 months were analysed by DIGE and immunoblotting. Results indicate changes in myosin binding protein C, aldehyde dehydrogenase, serpins and sirtuin-3 which protects from the opening of the mitoc...
Source: EuPA Open Proteomics - April 1, 2016 Category: Bioinformatics Source Type: research
Bioscope Group Hands-on Courses on Nano-based Proteomics
Publication date: Available online 28 March 2016 Source:EuPA Open Proteomics Author(s): H.M. Santos, C. Lodeiro, E. Oliveira, J.L. Capelo (Source: EuPA Open Proteomics)
Source: EuPA Open Proteomics - March 28, 2016 Category: Bioinformatics Source Type: research
The EuPA Standardization Initiative
Publication date: Available online 28 March 2016 Source:EuPA Open Proteomics Author(s): F. Canals, F. Elortza, A. Paradela, G. Corthals, Michelle Camenzuli, A. Muñoz, O. Schiltz, Anne Gonzalez de Peredo, A. Sickman, C. Borchers, Fernando J. Corrales (Source: EuPA Open Proteomics)
Source: EuPA Open Proteomics - March 28, 2016 Category: Bioinformatics Source Type: research
Public proteomics data: how the field has evolved from sceptical inquiry to the promise of in silico proteomics
Publication date: Available online 28 March 2016 Source:EuPA Open Proteomics Author(s): Lennart Martens Data sharing in the field of proteomics started from a rather negative perspective, with a focus on the validation of reported findings. Yet even from the very start, several repositories already focused on the much more positive aspect of data re-use, a strategy that is now pervasively adopted across all repositories and also ever more broadly applied in the field. While the enabling of results validation remains a core feature of data dissemination efforts, the much more positive focus on data re-use has combined...
Source: EuPA Open Proteomics - March 28, 2016 Category: Bioinformatics Source Type: research
Call for Candidates for the 2016 EuPA Young Investigator Prize (PhD)
Publication date: Available online 28 March 2016 Source:EuPA Open Proteomics (Source: EuPA Open Proteomics)
Source: EuPA Open Proteomics - March 28, 2016 Category: Bioinformatics Source Type: research
The EuPA Bioinformatics Community (EuBIC) initiative
Publication date: Available online 28 March 2016 Source:EuPA Open Proteomics Author(s): Marc Vaudel (Source: EuPA Open Proteomics)
Source: EuPA Open Proteomics - March 28, 2016 Category: Bioinformatics Source Type: research
Message from the President
Publication date: Available online 28 March 2016 Source:EuPA Open Proteomics Author(s): Fernando Corrales (Source: EuPA Open Proteomics)
Source: EuPA Open Proteomics - March 28, 2016 Category: Bioinformatics Source Type: research
EuPA News from the EuPA Conference and Communication Committee (CCC)
Publication date: Available online 28 March 2016 Source:EuPA Open Proteomics Author(s): Concha Gil, Martina Marchetti-Deschmann, Deborah Penque, Paola Roncada, Natacha Turck, Fernando J. Corrales (Source: EuPA Open Proteomics)
Source: EuPA Open Proteomics - March 28, 2016 Category: Bioinformatics Source Type: research
The Serbian Proteomics Association (SePA)
Publication date: Available online 28 March 2016 Source:EuPA Open Proteomics (Source: EuPA Open Proteomics)
Source: EuPA Open Proteomics - March 28, 2016 Category: Bioinformatics Source Type: research
Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin
Publication date: Available online 16 March 2016 Source:EuPA Open Proteomics Author(s): Elisa Maffioli, Federico Aletti, Fabiana Santagata, Armando Negri, Marco H. Santamaria, Frank A. De Lano, Erik B. Kistler, Geert W. Schmid-Schönbein, Gabriella Tedeschi A preliminary mass spectrometry based shotgun protocol was set up to compare the peptidome of plasma samples from healthy and hemorrhagic shock rats with the aim of verifying the possible role of uncontrolled proteolytic activity in circulatory shock. Since the hemorrhagic shock model requires heparin as anticoagulant, a preliminary experiment usin...
Source: EuPA Open Proteomics - March 17, 2016 Category: Bioinformatics Source Type: research
Proprotein convertase 1/3 inhibited macrophages: A novel therapeutic based on drone macrophages
Publication date: Available online 5 March 2016 Source:EuPA Open Proteomics Author(s): Marie Duhamel, Franck Rodet, Adriana Murgoci, Maxence Wisztorski, Robert Day, Isabelle Fournier, Michel Salzet We demonstrated here thanks to proteomic, that proprotein convertase 1/3 knockdown macrophages present all the characteristic of activated pro-inflammatory macrophages. TLR4 and TLR9 signaling pathways can be enhanced leading to the secretion of pro-inflammatory factors and antitumor factors. We can control their activation by controlling one enzyme, PC1/3. In a tumor context, PC1/3 inhibition in macrophages ma...
Source: EuPA Open Proteomics - March 7, 2016 Category: Bioinformatics Source Type: research
Metabolite extraction for high-throughput FTICR-MS-based metabolomics of grapevine leaves
Publication date: Available online 5 March 2016 Source:EuPA Open Proteomics Author(s): Marisa Maia, Filipa Monteiro, Mónica Sebastiana, Ana Patrícia Marques, António E.N. Ferreira, Ana Ponces Freire, Carlos Cordeiro, Andreia Figueiredo, Marta Sousa Silva In metabolomics there is an ever-growing need for faster and more comprehensive analysis methods to cope with the increase of biological studies. Direct infusion Fourier-transform ion cyclotron-resonance mass spectrometry (DI-FTICR-MS) is used in non-targeted metabolomics to obtain high-resolution snapshots of the metabolic state of a system. In an...
Source: EuPA Open Proteomics - March 7, 2016 Category: Bioinformatics Source Type: research
Use of a charge reducing agent to enable intact mass analysis of cysteine-linked antibody-drug-conjugates by native mass spectrometry
Publication date: Available online 4 March 2016 Source:EuPA Open Proteomics Author(s): Kamila J. Pacholarz, Perdita E. Barran Antibody-drug-conjugates (ADC) are a growing class of anticancer biopharmaceuticals. Conjugation of cysteine linked ADCs, requires initial reduction of mAb inter-chain disulfide bonds, as the drugs are attached via thiol chemistry. This results in the active mAb moiety being transformed from a covalently linked tetramer to non-covalently linked complexes, which hinders precise determination of drug load with LC-MS. Here, we show how the addition of the charge reducing agent triethylammonium ...
Source: EuPA Open Proteomics - March 5, 2016 Category: Bioinformatics Source Type: research
