Metabolite extraction for high-throughput FTICR-MS-based metabolomics of grapevine leaves

Publication date: Available online 5 March 2016 Source:EuPA Open Proteomics Author(s): Marisa Maia, Filipa Monteiro, Mónica Sebastiana, Ana Patrícia Marques, António E.N. Ferreira, Ana Ponces Freire, Carlos Cordeiro, Andreia Figueiredo, Marta Sousa Silva In metabolomics there is an ever-growing need for faster and more comprehensive analysis methods to cope with the increase of biological studies. Direct infusion Fourier-transform ion cyclotron-resonance mass spectrometry (DI-FTICR-MS) is used in non-targeted metabolomics to obtain high-resolution snapshots of the metabolic state of a system. In any metabolic profiling study, the establishment of an effective metabolite extraction protocol is paramount. We developed an improved metabolite extraction method, compatible with DI-FTICR-MS-based metabolomics, using grapevine leaves. This extraction protocol allowed the extraction of polar and non-polar compounds, covering all major classes found in plants and increasing metabolome coverage. Graphical abstract

Source: EuPA Open Proteomics - March 7, 2016 Category: Bioinformatics Source Type: research