Top-down proteomic characterization of daoy medulloblastoma tumor cell line

Publication date: Available online 1 April 2016 Source:EuPA Open Proteomics Author(s): Claudia Martelli, Luca D’Angelo, Marta Barba, Mirko Baranzini, Ilaria Inserra, Federica Iavarone, Federica Vincenzoni, Gianpiero Tamburrini, Luca Massimi, Concezio Di Rocco, Massimo Caldarelli, IreneMessana, Fabrizio Michetti, Massimo Castagnola, Wanda Lattanzi, Claudia Desiderio The proteome of the DAOY medulloblastoma cell line has been investigated by an LC-MS top-down platform. This approach, unlike the bottom-up one, allows identifying proteins and peptides in their intact forms, and disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that strongly characterized DAOY cell intact proteome. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research