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Methods for the genetic manipulation of marine bacteria
Publication date: May 2018Source: Electronic Journal of Biotechnology, Volume 33Author(s): Zahraa Zeaiter, Francesca Mapelli, Elena Crotti, Sara BorinAbstractGenetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications. This procedure can be applied to explore the biotechnological potential associated with environmental bacteria and to utilize the functional properties of specific genes when inserted into an appropriate host. In the past years, marine bacteria have received inc...
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Draft genomes and reference transcriptomes extend the coding potential of the fish pathogen Piscirickettsia salmonis
ConclusionsThis data has provided a refined genome annotation and also new insights on the transcriptional structures and coding potential of this fish pathogen.How to cite: Millar AD, Tapia P, Gomez FA, et al. Draft genomes and reference transcriptomes extend the coding potential of the fish pathogen Piscirickettsia salmonis. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.04.002. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria
ConclusionsThese results indicate that a possible mechanism for regulating biofouling could be through alteration of biofilm EPS and alteration of the epiphytic bacterial community associated with the microalga.How to cite: Infante, C.D., Castillo, F., Pérez, V., et al. Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria. Electron J Biotechnol 2018;33 https://doi.org/10.1016/j.ejbt.2018.03.002. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Increasing pinosylvin production in Escherichia coli by reducing the expression level of the gene fabI-encoded enoyl-acyl carrier protein reductase
ConclusionA reduction in the transcript level of fabI caused by the elimination of the -35 and upstream promoter sequences is a successful strategy to improve pinosylvin production in E. coli.How to cite: Salas-Navarrete, C., Hernández-Chávez, G., Flores, N., et al. Increasing pinosylvin production in Escherichia coli by reducing expression level of gene fabI-encoded enoyl-acyl carrier protein reductase. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.03.001. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Scaling-up fermentation of Escherichia coli for production of recombinant P64k protein from Neisseria meningitidis
ConclusionsThe methodology described herein, for the KLa/k scale-up fermentation criterion, allowed us to obtain the P64k protein at 50 l scale. A fermentation process for the production of P64k protein from N. meningitidis was established, a protein to be used in future vaccine formulations in humans.How to cite: Espinosa R, García J, Narciandi E, et al. Scaling-up fermentation of Escherichia coli for production of recombinant P64k protein from Neisseria meningitidis. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.03.004. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses
ConclusionThe xylanase from P. chrysogenum F-15 showed lower enzymatic activity on commercial xylan than on hemicellulose from agroindustry biomass and its biochemistry characteristics, such as stability at 40°C and pH from 4.0 to 10.0, shows the potential of this enzyme for application in food, feed, pulp and paper industries and for bioethanol production.How to cite: Terrone CC, Freitas C, Terrasan CRF, et al. Agroindustrial biomass for xylanase production by Penicillium chrysogenum: purification, biochemical properties and hydrolysis of hemicelluloses. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018....
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Removing the by-products acetic acid and NH4+ from the l-tryptophan broth by vacuum thin film evaporation during l-tryptophan production
ConclusionsThe acetic acid and NH4+ in the fermentation broth were quickly removed using the vacuum scraper concentrator, which reduced bacterial inhibition, enhanced bacterial activity, and improved the production of l-tryptophan and glucose conversion rate.How to cite: Xu Q, Bai F, Chen N, et al. Removing the by-products acetic acid and NH4+ from the l-tryptophan broth by vacuum thin film evaporation during l-tryptophan production. Electron J Biotechnol 2018; 33. https://doi.org/10.1016/j.ejbt.2018.04.003. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Biofiltration of trimethylamine in biotrickling filter inoculated with Aminobacter aminovorans
ConclusionsA biotrickling filter inoculated with A. aminovorans can remove efficiently the TMA in a gaseous stream. The elimination capacity of TMA can be negatively affected by H2S, but its effect is not notorious when it is forming part of a biofilm, due to its high specific consumption rate of TMA.How to cite: Aguirre A, Bernal P, Maureira D, et al. Biofiltration of trimethylamine in biotrickling filter inoculated with Aminobacter aminovorans. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.04.004. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1
Publication date: Available online 30 June 2018Source: Electronic Journal of BiotechnologyAuthor(s): Jian Xu, Yujing Zhu, Hanchen Li, Limei Chen, Wuxi Chen, Min Cui, Lina Han, Wenbo Hou, Demao LiAbstractAlanine mother liquor, a type of industrial waste from alanine fermentation, was used as a nitrogen source to produce docosahexaenoic acid (DHA) by Schizochytrium sp. B4D1. The results indicated that yeast extract could trigger the utilization of the alanine mother liquor. Additionally, the alanine can be quenched during the culture, which aids in DHA accumulation. The medium components were optimized via response surface m...
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Hydrolytic efficiency and isomerization during de-esterification of natural astaxanthin esters by saponification and enzymolysis
ConclusionCompared with saponification, enzymolysis with cholesterol esterase was recommended as a more accurate method for de-esterification of natural astaxanthin esters for further qualitative and quantitative HPLC analysis.How to cite: Su F, Xu H, Yang N, et al. Hydrolytic efficiency and isomerization during the process of de-esterifying natural astaxanthin esters using saponification and enzymolysis. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.002. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Melanoma transplants in “green” mice: Fluorescent cells in tumors are not equivalent to host-derived cells
ConclusionsIn a melanoma model in GFP mice, the detection of “green” cells in tumors was not equivalent to the detection of host-derived cells. Such “masking” was caused by a transient, but lasting, transfer of EGFP mRNA from the host's normal cells to tumor cells. Thus, an analysis of tumors postmortem by techniques that yield only a single snapshot can lead to incorrect interpretations and erroneous conclusions.How to cite: Garcia-Olmo D, Picazo MG, García-Olmo D. Melanoma transplants in “green” mice: fluorescent cells in tumors are not equivalent to host-derived cells. Electron J Biotechnol 2018;34. https:/...
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers
ConclusionsTwenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae
ConclusionsThe modified Gompertz model was expanded to facilitate prediction of products' formation and fructose fractions in all three fermentors. Such expansion will be beneficial in industrial applications.How to cite: Sulieman AK, Putra MD, Abasaeed AE, et al. Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.04.006. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Comparison of the phenolic contents and epigenetic and genetic variability of wild and cultivated watercress (Rorippa nasturtium var. aquaticum L.)
ConclusionsRelevant chemical, epigenetic, and genetic differences have emerged between wild and cultivated watercress. These differences can contribute to fingerprint and develop quality control tools for the integral and safety use and the commercialization of watercress. The richness of epialleles could support the development of tools to manipulate the watercress epigenome to develop high bioproduct–producing cultivars.How to cite: Gutiérrez-Velázquez MV, Almaraz-Abarca N, Herrera-Arrieta Y, et al. Comparison of the phenolic contents and the epigenetic and genetic variability of wild and cultivated watercress (Rorip...
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Heterologous expression and enhanced production of β-1,4-glucanase of Bacillus halodurans C-125 in Escherichia coli
ConclusionProduction of endo-1, 4 β-glucanase enzymes from B. halodurans increased several folds when cloned in pET vector and expressed in E. coli. To our knowledge, this is the first report of high-level expression and characterization of an endo-1, 4 β-glucanases from B. halodurans.How to cite: Zeeshan N, Naz S, Naz S et al. Heterologous expression and enhanced production of β-1,4-glucanase of Bacillus halodurans C-125 in E. coli. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.001. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research