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Productive infection and transduction by bacteriophage P1 in the species Salmonella bongori
ConclusionTherefore, bacteriophage P1 can be used as a tool for the genetic manipulation in the species S. bongori. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 11, 2019 Category: Biotechnology Source Type: research

Expression and purification of the transcription factor StMsn2 from Setosphaeria turcica in Escherichia coli
ConclusionsA new gene, named StMSN2, with 1617 bp ORF was cloned from S. turcica and characterized using bioinformatics methods. StMsn2 was expressed and purified in a prokaryotic system. A polyclonal antibody, named Msn2-2, against StMsn2 with high specificity was identified. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 8, 2019 Category: Biotechnology Source Type: research

Modeling of lactose enzymatic hydrolysis using Monte Carlo method
ConclusionsMonte Carlo modeling method can be used to model enzymatic reactions in a simple way for real applications. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 4, 2019 Category: Biotechnology Source Type: research

Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets
ConclusionsThis is a promising strain for producing prodigiosin. The prodigiosin hase potential in anticancer medicine exploration. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 27, 2019 Category: Biotechnology Source Type: research

A comparison of callus induction in 4 Garcinia species
ConclusionsFor all 4 species, the greatest amount of large nodular callus was observed in the TDZ treatments. White, friable callus was observed on most of the 2,4-D and picloram treatment groups. Most meta-topolin treatments resulted in minimal callus formation. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 27, 2019 Category: Biotechnology Source Type: research

Expression and purification of a transcription factor StMsn2 from Setosphaeria turcica in Escherichia coli
ConclusionsA new gene name StMSN2 with 1617 bp ORF was cloned from S. turcica and characterized using bioinformatics methods. StMsn2 was expressed and purified in a prokaryotic system. A polyclonal antibody named Msn2–2 against StMsn2 with high specificity was identified. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 26, 2019 Category: Biotechnology Source Type: research

Development of biodegradation process for poly(DL-lactic acid) degradation by crude enzyme produced by Actinomadura keratinilytica strain T16–1
ConclusionsThis is the first report demonstrating the scaling up of PLA degradation in a 5 L bioreactor and the evaluation of a potential method for enhancing PLA degradation efficiency. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 23, 2019 Category: Biotechnology Source Type: research

Release of formaldehyde during the biofiltration of methanol vapors in a peat biofilter inoculated with Pichia pastoris GS115
ConclusionsFormaldehyde is produced inside a biofilter when methanol is treated in a biofiltration system inoculated with Pichia pastoris. Biomass present in the reactor is capable of degrading the formaldehyde generated as the concentration of methanol decreases. However, high methanol loads can lead to the generation and release of formaldehyde into the environment. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 13, 2019 Category: Biotechnology Source Type: research

Polymorphism of bovine lipocalin-2 gene and its impact on milk production traits and mastitis in Holstein Friesian cattle
ConclusionsAlthough, the identified SNPs were not found to have any impact on the somatic cell count or the incidence of mastitis in cows, it seems that further research is necessary, covering a larger population of cattle, to confirm the association between lipocalin-2 and milk production traits and mastitis. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 13, 2019 Category: Biotechnology Source Type: research

Chlorella sorokiniana and Chlorella minutissima exhibit antioxidant potentials, inhibit cholinesterases and modulate disaggregation of β-amyloid fibrils
This study reveals the modulatory effects of C. sorokiniana and C. minutissima extracts on some mediators of Alzheimer's disease and provides insights into their potential benefits as functional food, nutraceutics or therapeutic agent for the management of this neurodegenerative disease. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 12, 2019 Category: Biotechnology Source Type: research

Improvement of the alkali stability of Penicillium cyclopium lipase by error-prone PCR
ConclusionsThis work provided the theoretical foundation and preliminary data for improving alkali stability of PCL to meet the industrial requirements, which is also beneficial to improving alkali-tolerance ability of other industrial enzymes via molecular modification. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 9, 2019 Category: Biotechnology Source Type: research

Insights into the microbial community structure of anaerobic digestion of municipal solid waste landfill leachate for methane production by adaptive thermophilic granular sludge
ConclusionsWith the assistance of adaptive thermophilic granular sludge, microbial community structure was more evenly distributed, while both of COD degradation rate and methane production was improved during anaerobic digestion of MSW landfill leachate. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 9, 2019 Category: Biotechnology Source Type: research

Antiproliferative activity of biomass extract from Pseudomonas cedrina
ConclusionsThis is the first report of P. cedrina associated with the stems of P. patula in Mexico, as well as the antiproliferative activity of extracts from this species of bacteria against human solid tumor cell lines. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 6, 2019 Category: Biotechnology Source Type: research

CRISPR/Cas9-mediated MSTN gene editing induced mitochondrial alterations in C2C12 myoblast cells
ConclusionThese findings indicate that MSTN regulates mitochondrial biogenesis and metabolism. This gene-editing cells provided favourable evidence for animal breeding and metabolic diseases. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 6, 2019 Category: Biotechnology Source Type: research

Chlorella sorokiniana and Chlorella minutissima exhibits antioxidant potentials, inhibits cholinesterases and modulates dis-aggregation of β-amyloid fibrils
This study reveals the modulatory effects of C. sorokiniana and C. minutissima extracts on some mediators of Alzheimer's disease and gives insights into their potential benefits as functional food, nutraceutics or therapeutic agent for the management of this neurodegenerative disease. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 2, 2019 Category: Biotechnology Source Type: research