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Genomic comparisons of Rhizobium species using in silico AFLP-PCR, endonuclease restrictions and ampylating enzymes
Conclusions The phylogenetic tree based on AFLP-PCR was slightly different from restriction endonucleases and PFGE based tree. Different post translational modifications (PTM's) were observed in the Rhizobium species and the most prevailing type of PTM was AMPylation with the GS-ATasE (GlnE). Another type of PTM's was also observed i.e. Hydroxylation followed by Sulfation with domains i.e. Asparagine_hydroxylase, Collagen_prolyl_lysyl_hydroxylase and Sulfotransferase. The deamidation type of PTM was present only in Rhizobium sp. NGR234. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 26, 2018 Category: Biotechnology Source Type: research

Poly(3-hydroxybutyrate) graft copolymer dense membranes for human mesenchymal stem cell growth
Conclusions It is concluded that the developed membrane can be properly used in is the restoration of skin tissue. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 25, 2018 Category: Biotechnology Source Type: research

Hydrolytic efficiency and isomerization during de-esterification of natural astaxanthin esters by saponification and enzymolysis
Conclusion Compared with saponification, enzymolysis with cholesterol esterase was recommended as a more accurate method for de-esterification of natural astaxanthin esters for further qualitative and quantitative HPLC analysis. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 22, 2018 Category: Biotechnology Source Type: research

Development of a direct transformation method by GFP screening and in vitro whole plant regeneration of Capsicum frutescens L.
Conclusions We have obtained an optimized protocol for the biolistic transformation of chili and in vitro regeneration of chili plantlets. The establishment of the protocols will provide a platform for molecular breeding and biological studies of the chili plants. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 18, 2018 Category: Biotechnology Source Type: research

Hydrolytic efficiency and isomerization during the process of de-esterifying natural astaxanthin esters using saponification and enzymolysis
Conclusion Compared with saponification, enzymolysis with cholesterol esterase was recommended as a more accurate method for de-esterification of natural astaxanthin esters for further qualitative and quantitative HPLC analysis. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 17, 2018 Category: Biotechnology Source Type: research

Effects of all-trans retinoic acid on goat dermal papilla cells cultured in vitro
Conclusion DPCs are the targets of ATRA in hair follicle, and ATRA negatively regulates hair growth via targeted suppression of cell viability and growth factor expression of goat DPCs. Through these observations, we offer a new mechanistic insight of the roles of ATRA in hair biology. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 15, 2018 Category: Biotechnology Source Type: research

Heterologous expression and enhanced production of β-1,4-glucanase of Bacillus halodurans C-125 in Escherichia coli
Conclusion Production of endo-1, 4 β-glucanase enzymes from B. halodurans increased several folds when cloned in pET vector and expressed in E. coli. To our knowledge, this is the first report of high-level expression and characterization of an endo-1, 4 β-glucanases from B. halodurans. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 11, 2018 Category: Biotechnology Source Type: research

The genetic diversity of wild and cultivated manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers
Conclusions Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 11, 2018 Category: Biotechnology Source Type: research

Comparison of the phenolic contents and epigenetic and genetic variability of wild and cultivated watercress (Rorippa nasturtium var. aquaticum L.)
Conclusions Relevant chemical, epigenetic, and genetic differences have emerged between wild and cultivated watercress. These differences can contribute to fingerprint and develop quality control tools for the integral and safety use and the commercialization of watercress. The richness of epialleles could support the development of tools to manipulate the watercress epigenome to develop high bioproduct–producing cultivars. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 10, 2018 Category: Biotechnology Source Type: research

Melanoma transplants in “green” mice: Fluorescent cells in tumors are not equivalent to host-derived cells
Conclusions In a melanoma model in GFP mice, the detection of “green” cells in tumors was not equivalent to the detection of host-derived cells. Such “masking” was caused by a transient, but lasting, transfer of EGFP mRNA from the host's normal cells to tumor cells. Thus, an analysis of tumors postmortem by techniques that yield only a single snapshot can lead to incorrect interpretations and erroneous conclusions. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 2, 2018 Category: Biotechnology Source Type: research

Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae
Conclusions The modified Gompertz model was expanded to facilitate prediction of products' formation and fructose fractions in all three fermentors. Such expansion will be beneficial in industrial applications. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 1, 2018 Category: Biotechnology Source Type: research

Comparison of the phenolic contents and the epigenetic and genetic variability of wild and cultivated watercress (Rorippa nasturtium var. aquaticum L.)
Conclusions Relevant chemical, epigenetic, and genetic differences have arisen between wild and cultivated watercress. These differences can contribute to fingerprint and develop quality control tools for the integral and safety use, and commercialization of watercress. The richness of epialleles could support the development of manipulation tools of watercress epigenome to develop highly bioproducts-producing cultivars. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 1, 2018 Category: Biotechnology Source Type: research

Biofiltration of trimethylamine in biotrickling filter inoculated with Aminobacter aminovorans
Conclusions A biotrickling filter inoculated with A. aminovorans can remove efficiently the TMA in a gaseous stream. The elimination capacity of TMA can be negatively affected by H2S, but its effect is not notorious when it is forming part of a biofilm, due to its high specific consumption rate of TMA. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 17, 2018 Category: Biotechnology Source Type: research

Removing the by-products acetic acid and NH4+ from the l-tryptophan broth by vacuum thin film evaporation during l-tryptophan production
Conclusions The acetic acid and NH4 + in the fermentation broth were quickly removed using the vacuum scraper concentrator, which reduced bacterial inhibition, enhanced bacterial activity, and improved the production of l-tryptophan and glucose conversion rate. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 12, 2018 Category: Biotechnology Source Type: research

Draft genomes and reference transcriptomes extend the coding potential of the fish pathogen Piscirickettsia salmonis
Conclusions This data has provided a refined genome annotation and also new insights on the transcriptional structures and coding potential of this fish pathogen. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 12, 2018 Category: Biotechnology Source Type: research