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Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6
ConclusionThe results clearly imply that the redundancy of catA genes increases the available amount of C12O in P. putida ND6, which would be beneficial for survival in challenging environments.How to cite: Li S, Qin K, Li H, et al. Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6. Electron J Biotechnol 2018;34. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Effects of all-trans retinoic acid on goat dermal papilla cells cultured in vitro
ConclusionDPCs are the targets of ATRA in the hair follicle, and ATRA negatively regulates hair growth by the targeted suppression of cell viability and growth factor expression of goat DPCs. Through these observations, we offer a new mechanistic insight into the roles of ATRA in hair biology.How to cite: Ma S, Zhou G, Chen Y. Effects of all-trans retinoic acid on goat dermal papilla cells cultured in vitro. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.004. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Development of a direct transformation method by GFP screening and in vitro whole plant regeneration of Capsicum frutescens L.
ConclusionsWe obtained an optimized protocol for the biolistic transformation of chili and in vitro regeneration of chili plantlets. The establishment of the protocols will provide a platform for molecular breeding and biological studies of chili plants.How to cite: Chee MYY, Lycett GW, Chin CF. Development of a direct transformation method by GFP screening and in vitro whole plant regeneration of Capsicum frutescens L. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.005. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Poly(3-hydroxybutyrate) graft copolymer dense membranes for human mesenchymal stem cell growth
ConclusionsIt is concluded that the developed membrane can be properly used in is the restoration of skin tissue.How to cite: González-Torres M, Sánchez-Sánchez R, Solís-Rosales SG, et al. Poly(3-hydroxybutyrate) graft copolymer dense membranes for human mesenchymal stem cell growth. Electron J Biotechnol 2018, 34; https://doi.org/10.1016/j.ejbt.2018.05.007. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Genomic comparisons of Rhizobium species using in silico AFLP-PCR, endonuclease restriction, and AMPylating enzymes
ConclusionsThe phylogenetic tree based on AFLP-PCR was slightly different from restriction endonuclease- and PFGE-based trees. Different PTMs were observed in the Rhizobium species, and the most prevailing type of PTM was AMPylation with the domain GS-ATasE (GlnE). Another type of PTM was also observed, i.e., Hydroxylation and Sulfation, with the domains Asparagine_hydroxylase and Collagen_prolyl_lysyl_hydroxylase and Sulfotransferase, respectively. The deamidation type of PTM was present only in Rhizobium sp. NGR234.How to cite: Qureshi MA, Pervez MT, Babar ME, et al. Genomic comparisons of Rhizobium species using in sili...
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

JcZFP8, a C2H2 zinc finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco
ConclusionsThe results of this study indicate that JcZFP8 may play a role in J. curcas plant phenotype through GA-related pathways. Our findings may help us to understand the genetic regulation of plant development in J. curcas and to accelerate breeding progress through engineering of the GA metabolic pathway in this plant.How to cite: Shi X, Wu Y, Dai T, et al. JcZFP8, a C2H2 zinc-finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.008. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Recombinant production, biochemical and in silico characterization of lactate dehydrogenase from Geobacillus thermodenitrificans DSM-465
ConclusionsHighlighting the conserved structural and functional domains of LDH from two entirely different species, study has graded potential inhibitory molecules on the basis of their binding affinities that can be applied for in vivo anticancer studies. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Type C feruloyl esterase from Aspergillus ochraceus: A butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system
ConclusionsType C feruloyl esterase from A. ochraceus is a butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Recombinant production, biochemical and in silico characterization of lactate dehydrogenase from Geobacillus thermodenitrificans DSM-465
Conclusions Highlighting the conserved structural and functional domains of LDH from two entirely different species, study has graded potential inhibitory molecules on the basis of their binding affinities that can be applied for in vivo anticancer studies. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 4, 2018 Category: Biotechnology Source Type: research

Type C feruloyl esterase from Aspergillus ochraceus: A butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system
Conclusions Type C feruloyl esterase from A. ochraceus is a butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 4, 2018 Category: Biotechnology Source Type: research

Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1
Publication date: Available online 30 June 2018 Source:Electronic Journal of Biotechnology Author(s): Jian Xu, Yujing Zhu, Hanchen Li, Limei Chen, Wuxi Chen, Min Cui, Lina Han, Wenbo Hou, Demao Li Alanine mother liquor, a type of industrial waste from alanine fermentation, was used as a nitrogen source to produce docosahexaenoic acid (DHA) by Schizochytrium sp. B4D1. The results indicated that yeast extract could trigger the utilization of the alanine mother liquor. Additionally, the alanine can be quenched during the culture, which aids in DHA accumulation. The medium components were optimized via response surf...
Source: Electronic Journal of Biotechnology - July 1, 2018 Category: Biotechnology Source Type: research

Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6
Conclusion The results clearly imply that the redundancy of catA genes increases the available amount of catechol 1,2-dioxygenase in Pseudomonas putida ND6, which would be beneficial to survival in challenging environments. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - June 10, 2018 Category: Biotechnology Source Type: research

Genomic comparisons of Rhizobium species using in silico AFLP-PCR, endonuclease restriction, and AMPylating enzymes
Conclusions The phylogenetic tree based on AFLP-PCR was slightly different from restriction endonuclease- and PFGE-based trees. Different PTMs were observed in the Rhizobium species, and the most prevailing type of PTM was AMPylation with the domain GS-ATasE (GlnE). Another type of PTM was also observed, i.e., Hydroxylation and Sulfation, with the domains Asparagine_hydroxylase and Collagen_prolyl_lysyl_hydroxylase and Sulfotransferase, respectively. The deamidation type of PTM was present only in Rhizobium sp. NGR234. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - June 5, 2018 Category: Biotechnology Source Type: research

JcZFP8, a C2H2 zinc finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco
Conclusions The results of this study indicate that JcZFP8 plays a role in J. curcas plant phenotype through GA-related pathways. Our findings may help us to understand the genetic regulation of plant development in J. curcas and to accelerate breeding progress through engineering of the GA metabolic pathway in this plant. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - June 5, 2018 Category: Biotechnology Source Type: research

JcZFP8, a C2H2 zinc-finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco
Conclusions The results of this study indicate that JcZFP8 plays a role in J. curcas plant form via GA-related pathways. Our findings may help us to understand the genetic regulation of plant development in J. curcas, and accelerate breeding progress through engineering of the GA metabolic pathway in this plant. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 26, 2018 Category: Biotechnology Source Type: research