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Hydrolytic efficiency and isomerization during de-esterification of natural astaxanthin esters by saponification and enzymolysis
ConclusionCompared with saponification, enzymolysis with cholesterol esterase was recommended as a more accurate method for de-esterification of natural astaxanthin esters for further qualitative and quantitative HPLC analysis.How to cite: Su F, Xu H, Yang N, et al. Hydrolytic efficiency and isomerization during the process of de-esterifying natural astaxanthin esters using saponification and enzymolysis. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.002. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Melanoma transplants in “green” mice: Fluorescent cells in tumors are not equivalent to host-derived cells
ConclusionsIn a melanoma model in GFP mice, the detection of “green” cells in tumors was not equivalent to the detection of host-derived cells. Such “masking” was caused by a transient, but lasting, transfer of EGFP mRNA from the host's normal cells to tumor cells. Thus, an analysis of tumors postmortem by techniques that yield only a single snapshot can lead to incorrect interpretations and erroneous conclusions.How to cite: Garcia-Olmo D, Picazo MG, García-Olmo D. Melanoma transplants in “green” mice: fluorescent cells in tumors are not equivalent to host-derived cells. Electron J Biotechnol 2018;34. https:/...
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers
ConclusionsTwenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae
ConclusionsThe modified Gompertz model was expanded to facilitate prediction of products' formation and fructose fractions in all three fermentors. Such expansion will be beneficial in industrial applications.How to cite: Sulieman AK, Putra MD, Abasaeed AE, et al. Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.04.006. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Comparison of the phenolic contents and epigenetic and genetic variability of wild and cultivated watercress (Rorippa nasturtium var. aquaticum L.)
ConclusionsRelevant chemical, epigenetic, and genetic differences have emerged between wild and cultivated watercress. These differences can contribute to fingerprint and develop quality control tools for the integral and safety use and the commercialization of watercress. The richness of epialleles could support the development of tools to manipulate the watercress epigenome to develop high bioproduct–producing cultivars.How to cite: Gutiérrez-Velázquez MV, Almaraz-Abarca N, Herrera-Arrieta Y, et al. Comparison of the phenolic contents and the epigenetic and genetic variability of wild and cultivated watercress (Rorip...
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Heterologous expression and enhanced production of β-1,4-glucanase of Bacillus halodurans C-125 in Escherichia coli
ConclusionProduction of endo-1, 4 β-glucanase enzymes from B. halodurans increased several folds when cloned in pET vector and expressed in E. coli. To our knowledge, this is the first report of high-level expression and characterization of an endo-1, 4 β-glucanases from B. halodurans.How to cite: Zeeshan N, Naz S, Naz S et al. Heterologous expression and enhanced production of β-1,4-glucanase of Bacillus halodurans C-125 in E. coli. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.001. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6
ConclusionThe results clearly imply that the redundancy of catA genes increases the available amount of C12O in P. putida ND6, which would be beneficial for survival in challenging environments.How to cite: Li S, Qin K, Li H, et al. Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6. Electron J Biotechnol 2018;34. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Effects of all-trans retinoic acid on goat dermal papilla cells cultured in vitro
ConclusionDPCs are the targets of ATRA in the hair follicle, and ATRA negatively regulates hair growth by the targeted suppression of cell viability and growth factor expression of goat DPCs. Through these observations, we offer a new mechanistic insight into the roles of ATRA in hair biology.How to cite: Ma S, Zhou G, Chen Y. Effects of all-trans retinoic acid on goat dermal papilla cells cultured in vitro. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.004. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Development of a direct transformation method by GFP screening and in vitro whole plant regeneration of Capsicum frutescens L.
ConclusionsWe obtained an optimized protocol for the biolistic transformation of chili and in vitro regeneration of chili plantlets. The establishment of the protocols will provide a platform for molecular breeding and biological studies of chili plants.How to cite: Chee MYY, Lycett GW, Chin CF. Development of a direct transformation method by GFP screening and in vitro whole plant regeneration of Capsicum frutescens L. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.005. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Poly(3-hydroxybutyrate) graft copolymer dense membranes for human mesenchymal stem cell growth
ConclusionsIt is concluded that the developed membrane can be properly used in is the restoration of skin tissue.How to cite: González-Torres M, Sánchez-Sánchez R, Solís-Rosales SG, et al. Poly(3-hydroxybutyrate) graft copolymer dense membranes for human mesenchymal stem cell growth. Electron J Biotechnol 2018, 34; https://doi.org/10.1016/j.ejbt.2018.05.007. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Genomic comparisons of Rhizobium species using in silico AFLP-PCR, endonuclease restriction, and AMPylating enzymes
ConclusionsThe phylogenetic tree based on AFLP-PCR was slightly different from restriction endonuclease- and PFGE-based trees. Different PTMs were observed in the Rhizobium species, and the most prevailing type of PTM was AMPylation with the domain GS-ATasE (GlnE). Another type of PTM was also observed, i.e., Hydroxylation and Sulfation, with the domains Asparagine_hydroxylase and Collagen_prolyl_lysyl_hydroxylase and Sulfotransferase, respectively. The deamidation type of PTM was present only in Rhizobium sp. NGR234.How to cite: Qureshi MA, Pervez MT, Babar ME, et al. Genomic comparisons of Rhizobium species using in sili...
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

JcZFP8, a C2H2 zinc finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco
ConclusionsThe results of this study indicate that JcZFP8 may play a role in J. curcas plant phenotype through GA-related pathways. Our findings may help us to understand the genetic regulation of plant development in J. curcas and to accelerate breeding progress through engineering of the GA metabolic pathway in this plant.How to cite: Shi X, Wu Y, Dai T, et al. JcZFP8, a C2H2 zinc-finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.008. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Recombinant production, biochemical and in silico characterization of lactate dehydrogenase from Geobacillus thermodenitrificans DSM-465
ConclusionsHighlighting the conserved structural and functional domains of LDH from two entirely different species, study has graded potential inhibitory molecules on the basis of their binding affinities that can be applied for in vivo anticancer studies. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Type C feruloyl esterase from Aspergillus ochraceus: A butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system
ConclusionsType C feruloyl esterase from A. ochraceus is a butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Evaluation of biogas and syngas as energy vectors for heat and power generation using lignocellulosic biomass as raw material
Publication date: May 2018Source: Electronic Journal of Biotechnology, Volume 33Author(s): Juan Camilo Solarte-Toro, Yessica Chacón-Pérez, Carlos Ariel Cardona-AlzateAbstractThe use of nonrenewable energy sources to provide the worldwide energy needs has caused different problems such as global warming, water pollution, and smog production. In this sense, lignocellulosic biomass has been postulated as a renewable energy source able to produce energy carriers that can cover this energy demand. Biogas and syngas are two energy vectors that have been suggested to generate heat and power through their use in cogeneration sys...
Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research