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Key microbial populations involved in anaerobic degradation of phenol and p-cresol using different inocula
ConclusionsSyntrophorhabdus and bamA gene play relevant roles in anaerobic degradation of phenolics. Estimation of these components could serve as a fast screening tool to find the most acclimatized sludge to efficiently degrade mono-aromatic compounds. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 25, 2018 Category: Biotechnology Source Type: research

Purification, characterization, mode of action and enhanced production of Salivaricin mmaye1, a novel bacteriocin from Lactobacillus salivarius SPW1 of human gut origin
ConclusionsA novel, cell-wall adhering, highly potent bacteriocin with a broad-spectrum of inhibitory activity, membrane-permeabilizing ability and enhanced production in a newly constituted medium has been isolated. It has a quorum sensing regulatory system and possesses interesting physico-chemical characteristics favoring its future use in food biopreservation. These findings pave the way for future evaluation of its medical and food applications. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 25, 2018 Category: Biotechnology Source Type: research

Downregulation of OsAGO17 by artificial microRNA causes pollen abortion resulting in the reduction of grain yield in rice
ConclusionThese results represent the first report of the biological function for OsAGO17 in rice and indicate that OsAGO17 may possibly play crucial regulatory roles in rice pollen development. It helps us to better understand the mechanism of pollen development in rice. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 28, 2018 Category: Biotechnology Source Type: research

Down-regulation of OsAGO17 by artificial microRNA caused pollen abortion, resulting in reduction of grain yield in rice
ConclusionThis results represent the first report of the biological function for OsAGO17 in rice and indicate thatOsAGO17 may possibly play crucial regulatory roles in rice pollen development. It helps us better understand the mechanism of pollen development in rice. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 17, 2018 Category: Biotechnology Source Type: research

Recombinant production and biochemical and in silico characterization of lactate dehydrogenase from Geobacillus thermodenitrificans DSM-465
ConclusionsBy highlighting the conserved structural and functional domains of LDH from two entirely different species, this study has graded potential inhibitory molecules on the basis of their binding affinities so that they can be applied for in vivo anticancer studies. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 15, 2018 Category: Biotechnology Source Type: research

Evaluation of biogas and syngas as energy vectors for heat and power generation using lignocellulosic biomass as raw material
Publication date: May 2018Source: Electronic Journal of Biotechnology, Volume 33Author(s): Juan Camilo Solarte-Toro, Yessica Chacón-Pérez, Carlos Ariel Cardona-AlzateAbstractThe use of nonrenewable energy sources to provide the worldwide energy needs has caused different problems such as global warming, water pollution, and smog production. In this sense, lignocellulosic biomass has been postulated as a renewable energy source able to produce energy carriers that can cover this energy demand. Biogas and syngas are two energy vectors that have been suggested to generate heat and power through their use in cogeneration sys...
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Methods for the genetic manipulation of marine bacteria
Publication date: May 2018Source: Electronic Journal of Biotechnology, Volume 33Author(s): Zahraa Zeaiter, Francesca Mapelli, Elena Crotti, Sara BorinAbstractGenetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications. This procedure can be applied to explore the biotechnological potential associated with environmental bacteria and to utilize the functional properties of specific genes when inserted into an appropriate host. In the past years, marine bacteria have received inc...
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Draft genomes and reference transcriptomes extend the coding potential of the fish pathogen Piscirickettsia salmonis
ConclusionsThis data has provided a refined genome annotation and also new insights on the transcriptional structures and coding potential of this fish pathogen.How to cite: Millar AD, Tapia P, Gomez FA, et al. Draft genomes and reference transcriptomes extend the coding potential of the fish pathogen Piscirickettsia salmonis. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.04.002. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria
ConclusionsThese results indicate that a possible mechanism for regulating biofouling could be through alteration of biofilm EPS and alteration of the epiphytic bacterial community associated with the microalga.How to cite: Infante, C.D., Castillo, F., Pérez, V., et al. Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria. Electron J Biotechnol 2018;33 https://doi.org/10.1016/j.ejbt.2018.03.002. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Increasing pinosylvin production in Escherichia coli by reducing the expression level of the gene fabI-encoded enoyl-acyl carrier protein reductase
ConclusionA reduction in the transcript level of fabI caused by the elimination of the -35 and upstream promoter sequences is a successful strategy to improve pinosylvin production in E. coli.How to cite: Salas-Navarrete, C., Hernández-Chávez, G., Flores, N., et al. Increasing pinosylvin production in Escherichia coli by reducing expression level of gene fabI-encoded enoyl-acyl carrier protein reductase. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.03.001. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Scaling-up fermentation of Escherichia coli for production of recombinant P64k protein from Neisseria meningitidis
ConclusionsThe methodology described herein, for the KLa/k scale-up fermentation criterion, allowed us to obtain the P64k protein at 50 l scale. A fermentation process for the production of P64k protein from N. meningitidis was established, a protein to be used in future vaccine formulations in humans.How to cite: Espinosa R, García J, Narciandi E, et al. Scaling-up fermentation of Escherichia coli for production of recombinant P64k protein from Neisseria meningitidis. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.03.004. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses
ConclusionThe xylanase from P. chrysogenum F-15 showed lower enzymatic activity on commercial xylan than on hemicellulose from agroindustry biomass and its biochemistry characteristics, such as stability at 40°C and pH from 4.0 to 10.0, shows the potential of this enzyme for application in food, feed, pulp and paper industries and for bioethanol production.How to cite: Terrone CC, Freitas C, Terrasan CRF, et al. Agroindustrial biomass for xylanase production by Penicillium chrysogenum: purification, biochemical properties and hydrolysis of hemicelluloses. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018....
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Removing the by-products acetic acid and NH4+ from the l-tryptophan broth by vacuum thin film evaporation during l-tryptophan production
ConclusionsThe acetic acid and NH4+ in the fermentation broth were quickly removed using the vacuum scraper concentrator, which reduced bacterial inhibition, enhanced bacterial activity, and improved the production of l-tryptophan and glucose conversion rate.How to cite: Xu Q, Bai F, Chen N, et al. Removing the by-products acetic acid and NH4+ from the l-tryptophan broth by vacuum thin film evaporation during l-tryptophan production. Electron J Biotechnol 2018; 33. https://doi.org/10.1016/j.ejbt.2018.04.003. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Biofiltration of trimethylamine in biotrickling filter inoculated with Aminobacter aminovorans
ConclusionsA biotrickling filter inoculated with A. aminovorans can remove efficiently the TMA in a gaseous stream. The elimination capacity of TMA can be negatively affected by H2S, but its effect is not notorious when it is forming part of a biofilm, due to its high specific consumption rate of TMA.How to cite: Aguirre A, Bernal P, Maureira D, et al. Biofiltration of trimethylamine in biotrickling filter inoculated with Aminobacter aminovorans. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.04.004. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1
Publication date: Available online 30 June 2018Source: Electronic Journal of BiotechnologyAuthor(s): Jian Xu, Yujing Zhu, Hanchen Li, Limei Chen, Wuxi Chen, Min Cui, Lina Han, Wenbo Hou, Demao LiAbstractAlanine mother liquor, a type of industrial waste from alanine fermentation, was used as a nitrogen source to produce docosahexaenoic acid (DHA) by Schizochytrium sp. B4D1. The results indicated that yeast extract could trigger the utilization of the alanine mother liquor. Additionally, the alanine can be quenched during the culture, which aids in DHA accumulation. The medium components were optimized via response surface m...
Source: Electronic Journal of Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research